No abstract available.
Alendronate* ; Animals ; Rats*

To evaluate the clinical aspects of central serous chorioretinopathy, the patients were analysized and divided into three groups: group I) initial visitants; group II) those who undertook fluorescein angiography; and group III) those who had been followed over 3 months. The overall frequency of this disease was 0.69%. In group I, of 262 patients, 76.5% was male, 85.5% in the forth and fifth decades and 7.7% bilateral. In group II, of 130 eyes (120 patients), only neurosensory retina was detached in 121 eyes. Of which 106 eyes (87.6%) which had ink blot leakage and 95 eyes (78.5%) had one leakage point. In 96 eyes (79.3%), leakage point located within one disc from the fovea. In group III, of 105 eyes (95 patients), initial visual acuity was better than 0.7 in 58 eyes (55.2%) and worse than 0.3 in 14 eyes (13.3%). Final visual acuity was better than 0.7 in 94 eyes (89.5%) and worse than 0.3 in 4 eyes (4.0%). In 34 eyes which were laser-treated, duration of recovery (10.8 vs 6.4 weeks; P=0.016) and frequency of recurrence (42.3 vs 17.6%; P=0.013) decreased to a statistically significantly compared with 71 conservatively treated eyes. Central serous chorioretinopathy has the high possibility of recurrence and therefore should be followed up periodically for possible need for laser photocoagulation.
Central Serous Chorioretinopathy ; Fluorescein Angiography ; Humans ; Ink ; Laser Therapy ; Light Coagulation ; Male ; Recurrence ; Retina ; Visual Acuity

Purpose of the present study was to find the optimal ovulation induction medicine for the maturation and development of immature oocytes and culture media for 2-cell embryos in the mouse model. ICR female mouse aged 6 to 8 weeks, were stimulated with 5 IU PMSG injection. At 47 to 50 hour post-PMSG injection, ovaries were dissected out and oocytes-cumulus complexes were punctured. The oocyte-cumulus complexes were cultured in media containing various ovulation induction medicine, CC, HMG and Metrodin for 18 hours. Female ICR mice were stimulated with 5 IU PMSG and 48 hours later were injected 5 IU of hCG, then female and male mice were mated. At 48 hour post-hCG injection, oviducts were dissected out and 2-cell embryos were flushed. The 2-cell embryos were cultured in various media, Ham's F-10 media of milli-Q water (3degrees), Ham's F-10 media of HPLC (high performance liquid chromatography, Baxter) water, Medicult media, HTF (human tubal fluid) media for 96hours. The results were as follows. 1. When the oocytes-cumulus complexes were cultured in 10(-9)microgram/ml~ 10(-8)microgram/ml of CC, those were suppressed in meiotic maturation (28.2~ 33.7%). Whereas the oocytes-cumulus complexes were cultured in 10(-7)microgram/ml~10(-4)microgram/ml, these were not effected in meiotic maturation (54.5~72.7%). 2. When the oocytes-cumulus complexes were cultured in 10(-4)microgram/ml~ 10(-1)microgram/ml of Metrodin, those were suppressed in meiotic maturation (35.7~ 41.5%). Meanwhile the oocytes-cumulus complexes were cultured in 10(-7)microgram/ml~10(-5)microgram/ml, those were not effected in meiotic maturation (54.2~ 70.3%). 3. When the oocytes-cumulus complexes were cultured in 10(-5)microgram/ml~ 10(-4)microgram/ml of HMG, those were suppressed in meiotic maturation (48.2~ 50.4%). As being cultured in 10(-7)microgram/ml~10(-6)microgram/ml, increased in meiotic maturation (75.8~80.7%). 4. When the 2-cell embryos were cultured in Ham's F-10 media of milli-Q wats. ( 3degrees), Ham's F-10 media of HPLC (high performance liquid chromatograpy, Banter) water, Medicult media, HTF (human tubal fluid) media, developmental rates to blastocyst and hatching for 96 hour were 50.0%, 45.2%, 71.5% and 95.6%, respectively.
Animals ; Blastocyst ; Chromatography, High Pressure Liquid ; Chromatography, Liquid ; Culture Media ; Embryonic Structures* ; Female ; Humans ; Male ; Mice* ; Mice, Inbred ICR ; Oocytes* ; Ovary ; Oviducts ; Ovulation Induction* ; Ovulation* ; Urofollitropin ; Water