Cell cycle associated nuclear proteins such as proliferating cell nuclear antigen(PCNA), argyrophilic nucleolar organizer regions(AgNORs) and the family of nuclear proteins identified by the Ki-67 epitope, have been primarily utilized for estimating of growth potential of neoplasms. Although PCNA and AgNORs staining are possible in the paraffin-embedded tissue, Ki-67 staining had been only possible on frozen sections. Recently monoclonal antibody MIB-1 is available, and reacts with the Ki-67 epitope in paraffin-embedded tissue. Twenty eight astrocytic tumors in paraffin-embedded, archival materials were stained by immunohistochemical technique for the MIB-1, PCNA, and by silver colloid stain for AgNORs. The MIB-1 labeling indicies(LI) ranged from 2 to 25%(10+/-7.58) for 10 glioblastomas; from 2 to 15%(7+/-3.74) for 11 anaplastic astrocytomas; and from 1 to 5%(3+/-1.91) for low grade astrocytomas. Glioblastomas and anaplastic astrocytomas exhibited significantly higher MIB-1 LI than their benign counterparts(p<0.05). The AgNORs count per cell ranged from 1.3 to 3.1(1.96+/-0.57) for 10 glioblastomas: from 1.2 to 3.1(1.9+/-0.64) for 11 anaplastic astrocytomas: and from 0.8 to 1.5(1.2+/-0.26) for low grade astrocytomas. Glioblastomas and anaplastic astrocytomas exhibited significantly higher AgNORs count than their benign counterparts(p<0.05). The PCNA LI ranged from 10 to 40%(24.5+/-10.39) for 10 glioblastomas; from 5 to 20%(11.6+/-5.24) for 11 anaplastic astrocytomas; and from 5 to 10%(7.1+/-2.67) for low grade astrocytomas. The differences of PCNA LI between glioblastomas and anaplastic astrocytomas(p<0.01), and between glioblastomas and low grade astrocytomas(p<0. 001) were statistically significant. Linear regression analysis showed correlations between MIB-1 LI and AgNORs count(Spearmans r=0.4306, p<0.05), between PCNA LI and AgNORs count(Spearman's r=0.586, p<0.05) and between PCNA and MIB-1 LI(Spearman's r=0.4523, p<0.05). These findings suggest that LI of MIB-1, PCNA and AgNORs count are correlated each other, and can be used as helpful markers for differentiating astrocytic tumors in addition to conventional staining methods.
Astrocytoma ; Cell Cycle ; Colloids ; Frozen Sections ; Glioblastoma ; Humans ; Linear Models ; Nuclear Proteins ; Nucleolus Organizer Region ; Proliferating Cell Nuclear Antigen* ; Silver

No abstract available.
Lip*

No abstract available.
Carcinoma, Squamous Cell* ; Porokeratosis*

No abstract available.
Colitis, Ulcerative* ; Ulcer*

Radiological investigation in patients with clonorchiasis is very important as this is the only method of evaluating the severity of clonorchiasis. In order to correlate the radiologic and pathologic findings of clonorchiasis, fourteen rabbits infested with Clonorchis sinensis and five control rabbits were examined radiologically by ultrasonography, computed tomography and cholangiography and the results were correlated with pathologic findings. Dilatation of the intrahepatic small bile ducts of the liver was due to obstruction by flukes: oval or elliptical small filling defects or irregular margin of the bile ducts on cholangiogram or intraluminal echoes on sonogram represented flukes per se; periductal thickening on sonogram and periductal enhancement of bile ducts on CT were due to inflammatory cell infiltration, adenomatous hyperplasia and periductal fibrosis: band like enhancement at the periphery of the liver on CT represented proliferated bile ducts, destruction of liver cells and resultant fibrosis. The study confirmed the pathological bases for the radiological findings of clonorchiasis in liver and bile ducts and will, perhaps, serve as a basis for the future radiologic-pathological correlation of clonorchiasis and in further clinical and experimental researches in the biliary tract diseases.
Bile Ducts ; Biliary Tract Diseases ; Cholangiography ; Clonorchiasis* ; Clonorchis sinensis ; Dilatation ; Fibrosis ; Humans ; Hyperplasia ; Liver ; Methods ; Rabbits* ; Trematoda ; Ultrasonography

No abstract available.
Paget Disease, Extramammary*

No abstract available.
Lichen Planus*

No abstract available.
Pregnancy, Heterotopic*

No abstract available.
Granuloma Annulare* ; Granuloma* ; Herpes Zoster*

OBJECTIVE: The aim of this study was to evaluate the relationship between sustained attention deficits in Attention-Deficit/Hyperactivity Disorder(ADHD) children and short-term Heart Rate Variability(HRV) parameters. In addition, we evaluate the relationship between The ADHD rating scale(ARS), the computerized ADHD diagnostic system(ADS) and Parenting stress index-short form(PSI-SF). METHODS: This study was performed in the department of children and Adolescent psychiatry, Korea university Guro hospital from august 2008 to January 2009. We evaluated HRV parameters by short-term recordings of 5 minutes. K-ARS and ADS are used for screening and identifying ADHD children. Intelligence was measured using Korean educational Developmental Institute-wechsler Intelligence Scale for Children. The caregivers Complete Parenting Stress Index scale for evaluation parent stress. RESULTS: The low frequency(LF) was significantly correlated with response variability of ADS. However, the other variables of ARS and ADS were not significantly correlated with LF. Hyperactivity subscale of ARS was significantly correlated with parental distress subscale and difficult child subscale of PSI-SF and inattention subscale of ARS was also significantly correlated with dysfunctional interaction and difficult child subscale of PSI-SF. CONCLUSION: The LF, 0.10-Hz component of HRV is known to measure effort allocation. This study shows that the LF component of HRV is significantly correlated with the response variability of ADS. This means that more severe symptoms of ADHD were correlated with the increase in the LF that means decreased effort allocation. These results also support the clinical usability of HRV in the assessment of ADHD. Furthermore, PSI-SF is correlated with hyperactivity and inattention variables of ARS.
Adolescent ; Adolescent Psychiatry ; Caregivers ; Child ; Heart ; Heart Rate ; Humans ; Intelligence ; Korea ; Mass Screening ; Parenting ; Parents