An experimental of Vibrio vulnificus infection has been performed with the intravenous, subcutaneous and oral inoculation of Vibrio vulnificus into ICR mice. The results are as follows: 1) The LD50 of the intravenous, subcutaneous and oral inoculation of Vibrio vulnificus were 1.6x10(7) cells/ml, 4.0x10(7) cells/ml, and 2.5x10(9) cells/ml, respectively. 2) In the experimental group without treatment with CC14, the survival rates for intravenous inoculation were 100% (1/2 LD50), 39.1% (LD50), and 8.3% (2 LD50). The survival rates for subcutaneous inoculation groups were 100% (1/2 LD50), 46.9% (LD50), and 18.8% (2 LD50). And the survival rates for oral inoculation groups were 100% (1/2 LD50), 53.1% (LD50), and 43.8% (2 LD50). 3) In those treated with CC14 0.05 ml, the survival rates for intravenous inoculation groups were 43.8% (1/2 LD50), 29.1% (LD50), 0% (2 LD50). The survival rates for subcutaneous inoculation groups were 59.4% (1/2 LD50), 40.6% (LD50), and 9.4% (2 LD50). The survival rates for oral inoculation groups were 68.8% (1/2 LD50), 46.9% (LD50), and 18.8% (2 LD50). In those treated with CC14 0.1 ml, the survival rates for intravenous inoculation groups were 25.0% (1/2 LD50), 10.4% (LD50), and 0% (2 LD50). The survival rates for subcutaneous inoculation groups were 43.8% (1/2 LD50), 21.9% (LD50), 0% (2 LD50). The survival rates for oral inoculation groups were 50.0% (1/2 LD50), 37.5% (LD50), and 0% (2 LD50). 4) Liver, lungs, meninges and brain, kidneys, heart, gastrointestinal tract and spleen showed septic inflammatory findings. Their degree of inflammation were different according to the severity of hepatic damage and the inoculum size.
Mice ; Animals

No abstract available.
Finite Element Analysis*

Recently there is increasing tendency of the nosocomial infection, and Pseudomonas aeruginosa is one of the most important and common pathogens causing hospital opportunistic infections with rapid emergence of resistant strain especially in immunologically compromised patients. An experimental study for the effects of polyvalent Pseudomonas vaccine was performed in an animal model of Pseudomonas sepsis on a survival rates and histopathological points of view-using ICR inbred mice. The vaccine was prepared with heat killed whole cells of the 10 representative serotypes of Pseudomonas aeruginosa, which were isolated from the Department of Microbiology, College of Medicine, Kyung Hee University and Seoul National University, and they were devided into two polyvalent vaccine groups. The animal model of the Pseudomonas sepsis was deveoped by intravenous inoculation of Pseudomonas aeruginosa (serotype F, inoculum size 100 microliter, 109 cells/ml), immediately after cutaneous burns. The results were as follows. 1) The survival rate of the immune mice was 100% and that of non-immune mice was 60%. 2) The histologic findings of lung of the non-immune mice were severe congestion (18/18 mice), hemorrhage (18/18 mice), emphysematous change (18/18 mice), thrombosis (9/18 mice), infarction (9/18 mice) and inflammation (6/18 mice) and those of the immune mice were only congestion (6/20 mice) and focal emphysematous change (2/20) from the 3 day experimental group. 3) The histologic findings of the liver in the non-immune mice were severe congestion, Kupffer cell mobilization, focal necrosis, & portal inflammation in most of them, and from 7 day experimental group there were noted infiltrations of lagre histiocytic cells in sinusoids, and those in the immune mice were only reactive change of varying degree. 4) The histologic findings of the spleen in the non-immune mice were severe reactive hyperplasia in all and ischemic necrosis in about half of them, and those in the immune mice were only reactive change. 5) The histologic findings of the heart in the non-immune mice were severe congestion and inflammation in most and in the immune mice were only occasional nonspecific congestion. 6) The histologic findings of the kidney in the non-immune mice were severe congestion in all, interstitial inflammation, acute tubular necrosis and cortical necrosis in about half of them, and those in the immune mice were only mild congestion. With the above results, we can suspect there is a significant protective effects of the polyvalent pseudomonas vaccine on the pseudomonas sepsis in ICR mice.
Mice ; Animals

No abstract available.
Adolescent* ; Child* ; Cushing Syndrome* ; Humans

Extra-adrenal pheochromocytoma accounts for about 10 to 15 percent of all such tumors and the combination of pheochromocytoma and renal artery stenosis is rare. We report an unusual case of extra-adrenal pheochromocytoma causing renal artery stenosis which was developed in a 11-year-old boy and the literatures were reviewed.
Child ; Humans ; Male ; Pheochromocytoma* ; Renal Artery Obstruction* ; Renal Artery*

Six cases of lunate dislocation were treated in the departments of orthopaedic surgery, Hangang Sacred Heart Hospital and Kangnam Sacred Heart Hospital, Hallym College during the period from March 1, 1978 to August 31, 1982. The results were as follows; l. Among the total 6 cases, two cases had lunate dislocation only and the other four had associated injuries of the same wrist joints. 2. Marupulative reduction was successful in only one case. Five cases were treated by open reduction, and for four of them internal fixation was tried. 3. Postoperative roentgenograms showed acceptable reductions in all cases except one which redislocation occurred. This case showed lunate dorsiflexion instability and was treated with extensor carpi radialis longus tenodesis. 4. The cases with pure lunate dislocation yielded better functional recovery than those with associated carpal injuries.
Dislocations ; Heart ; Tenodesis ; Wrist Joint

Colloid Milium is a degenerative disease in the dermal connective tissue characterized by the development of yellowish translucent papules or plaques on sun exposed skin. Clinically, there are two types of colloid milium namely, a juvenile type which starts before puberty and an adult type which involves sun exposed areas of adults. In both types the lesion is limited to the skin. Histologically colloid milium, like amyloid, shows homogenous, fissured aggregates, staining faintly eosinophilic, and occupying most or all of the involved. dermal papillae. A case of colloid. milium in 46 year old female is reported. About 4 years ago numerous yellowis4 translucent papules, 0. 1~0. 2cm in diameter developei on nose and lateral aspects of both eyes, which were gradually increasing in its size. Diagnosed confirmed by clinical features and histopathologic finding. Literature were reviewed.
Adolescent ; Adult ; Amyloid ; Colloids ; Connective Tissue ; Eosinophils ; Female ; Humans ; Middle Aged ; Nose ; Puberty ; Skin ; Solar System

Crohns disease is a granulomatous isease of the gastrointetin 1 tract, especially terminal ileum, but may involve the lip, mouth, anus, skin, liver and bone. The skin is a common site of extraintestinal involvement in Crohns disease, with the perianil, perineal, and peristomal areas most frequently affected. Vulvar involvement, however, is an uncommon extraintestinal manifestantion of Crohns disease. We report a case of a 16-year-old girl with Crohns disease of the vulva and perianal region. A histopathologic study of the descending colon and vulvar skin showed noncaseating granulomatous reactions composed of lymphocytes, epithelioidell and giant cells. We were able to make the diagnosis of Crohns disease of the vulvar and the patient was treated with prednisolone and metronidazole.
Adolescent ; Anal Canal ; Colon, Descending ; Crohn Disease* ; Diagnosis ; Female ; Giant Cells ; Humans ; Ileum ; Lip ; Liver ; Lymphocytes ; Metronidazole ; Mouth ; Prednisolone ; Skin ; Vulva*

No abstract available.
Abdomen, Acute* ; Nephritis* ; Purpura, Schoenlein-Henoch*

Two Cases of Juvenile XanthogranuIoma(JXG) are reported with review of literatures. JXG is a rare disease, and have not yet been reported in this country. Case I: Two months oId healthy female infant has two firm, discrete, brownish yellow waxy nodules on the left postauricular region and the left forearm. The lesion had begun as erythematous pinhead sized papules, and have been enlarged rapidly to pea siez during last three weeks. Case II: Three year old healthy male has firm, discrete, pinhead to pea sized scattered 16 papules and nodules on the trunk and right side of neck. Laboratory findmgs and X-ray studies were normal in both cases and there were no evidence of metabolie disorder or bony abnormality. Histopathologic studies show similar findings in both cases. In hematoxylin-eosin staining, epidermis shows parekeratosis and slight elongation of rete ridges with suprapapillary thinning with focal spongiosis and invasion of inflammatory cells. The entire dermis, from dermal papillae to sbcutis, is replaced by granulomatous lesion composed with histioeytes and a few giant cell reaction, in addition to histiocytic proliferation, considerable amount of eosinophils are infiltrated with some lymphocytes and plasma cells. The dermal collagen fibers are partly degenerated. In the upper most portion, some foamy histiocytes are also seen. In frozen section and Sudan III fat staining, the tissue shows rnild reactivities.
Collagen ; Dermis ; Eosinophils ; Epidermis ; Female ; Forearm ; Frozen Sections ; Giant Cells ; Histiocytes ; Humans ; Infant ; Lymphocytes ; Male ; Neck ; Peas ; Plasma Cells ; Rare Diseases ; Sudan ; Xanthogranuloma, Juvenile*