1.Attenuation of Vasoconstriction by Estrogen Through Endothelium -Independent Mechanism in Human Uterine Artery.
Byeong Jun JUNG ; Hyoung Moo PARK ; Min HUR ; Moo Yeool LEE
Korean Journal of Obstetrics and Gynecology 1999;42(8):1733-1743
OBJECTIVE: This study was performed to investigate whether 1) estrogen induces the change of vascular tone in endothelium-denuded human uterine artery, and 2) endothelium-independent vascular reactivity may be mediated by intracellular calcium ion modulation. METHODS: The uterine arteries from 34 premenopausal women were obtained at the time of hysterectomy due to various indications and endothelium was denuded. Vascular reactivity was monitored by using Isometric force transducer and recorded by physiograph. Endothelial integrity was assessed by sequential administration of 1micrometer norepinephrine(alpha-adrenergic stimulant) and 10 micrometer acetylcholine (endothelium -dependent vasorelaxant). Integrity of smooth muscle was measured by administration of 10 micrometer sodium nitroprusside (endothelium - independent vasorelaxant) and 10 micrometer tamsulosin(alpha-adrenergic blocker). A dose-dependent action of estrogen was measured on denuded uterine arteries, pretreated with norepinephrine and potassium chloride. In media contained denuded uterine arteries pretreated with 70mM potassium chloride and estradiol(3X10-5M), nitric oxide and its inhibitor, N-nitro-L-arginine methyl ester(L-NAME) was administered, respectively, in order to verify the vasodilation effect. Statistical tests were performed at the 5% level of significance using the SPSS PC+ package. RESULTS: Acethylcholine has little effect but sodium nitroprusside & talmsulosin showed marked relaxation, which suggested loss of endothelial function and adequacy of smooth muscle function. The contraction by norephinephrine(1 M) revealed estrogen induced relaxation which was concentration-dependent from 3 10-8M to 3 10-5M in concentration of 17 -estradiol. The contraction by high potassium solution 70mM was also inhibited by estrogen in concentration-dependent manner. This vasorelaxant effect of estrogen on endothelium-denuded uterine artery was not affected by addition of nitric oxide(NO) precursor, L-arginine and nitric oxide synthase(NOS) inhibitor, L-NAME. CONCLUSION: The results of this study revealed that estrogen has vasorelaxant effect on endothelium endothelium-denuded uterine artery by calcium antagonistic property through direct inhibitory effect on receptor- and voltage dependent calcium ion channels of smooth muscles. This vasorelaxant effect of estrogen on endothelium-denuded uterine artery was NO independent.
Acetylcholine
;
Arginine
;
Calcium
;
Calcium Channels
;
Endothelium*
;
Estrogens*
;
Female
;
Humans*
;
Hysterectomy
;
Muscle, Smooth
;
NG-Nitroarginine Methyl Ester
;
Nitric Oxide
;
Nitroprusside
;
Norepinephrine
;
Potassium
;
Potassium Chloride
;
Relaxation
;
Transducers
;
Uterine Artery*
;
Vasoconstriction*
;
Vasodilation
2.An experience with a free vascularized myo-osteochondral rib graft for mandibular reconstruction.
Jong Ho LEE ; Ku Jong SEO ; Kwang PARK ; Moo Kang JUNG ; Gee Duk PARK ; Jung Jae JEONG
Journal of the Korean Association of Oral and Maxillofacial Surgeons 1993;19(2):115-124
No abstract available.
Mandibular Reconstruction*
;
Ribs*
;
Transplants*
3.A Clinical and Laboratory Study on Infection in Childhood Leukemia.
Jung Hee LEE ; Kwang Hyun KIM ; In Joon SEOL ; Heung Jae LEE ; Hahng LEE ; Chong Moo PARK
Journal of the Korean Pediatric Society 1986;29(7):1-11
No abstract available.
Leukemia*
4.A Clinical Study on Reye's Syndrome.
Hye Sun JUNG ; Seong Ryong HYON ; Ha Baik LEE ; Soo Jee MOON ; Chong Moo PARK
Journal of the Korean Pediatric Society 1985;28(8):783-794
No abstract available.
Reye Syndrome*
5.Clinical study of hysterosalpingography in infertile women.
Jung Ok REW ; Byung Sun KIM ; Dong Keun KIM ; Hye Ryun OH ; Chan Moo PARK
Korean Journal of Obstetrics and Gynecology 1992;35(11):1621-1629
No abstract available.
Female
;
Humans
;
Hysterosalpingography*
6.Aggressive Osteoblastoma.
Yong Koo PARK ; Hyung Bae MOON ; Dong Kyuen LEE ; Dae Moo SHIM ; Jung Dal LEE
Korean Journal of Pathology 1986;20(2):235-239
Osteoblastomas were originally thought to be lesions that could be controlled with minimal local therapy, including curettage. However, at least some of the osteoblastomas with an unexpectedly active clinical course had histologic features which differed from ordinary osteoblastomas, and the term aggressive osteoblastoma was given to these lesions. In the aggressive osteoblastoma the osteoblasts are cytologically atypical. In addition, the well organized trabecular patterns of typical osteoblastoma is partly lacking, especially in the areas that are rich in atypical osteoblasts. The case report deals a case of aggressive osteoblastoma of the left iliac wing in a 44 year old male. The interesting radiologic manifestationa(plain x-ray CAT and radionuclide bone scan) and characteristic histologic findings are presented along with review of pertinent literatures.
7.Distinct Expressions of TGF-a among Chronic Hepatitis, Liver Cirrhosis, and Hepatocellular Carcinoma.
Byeong Moo YOO ; Sung Soo PARK ; Dong Hoo LEE ; Jung Dal LEE
The Korean Journal of Hepatology 1997;3(4):316-328
BACKGROUND/AIMS: Transforming growth factor-a(TGF-a) is a polypeptide cytokine related to cell proliferation and transformation. TGF-a binds to EGF receptor and stimulating DNA synthesis in liver cell. The hepatitis B virus (HBV) by itself is also believed to play a role in the hepatic carcinogenesis. Recently, it was reported that TGF-a and HBV were synergistic in action with rapid appearance of hepatocelluar carcinoma in bitransgenic mice. Although TGF- a is thought to play an important role in hepatocarcinogenesis, its expression during the natural history of HBV hepatitis was poorly understood. This investigation was performed to elucidate the dynamic changes and istinct immunohistochemical staining patterns in the course of chronic HBV hepatitis with specific reference to hepatocelluar carcinoma and to explain the role of TGF-a in the pathogenesis of hepatocelluar carcinoma. MATERIALS/METHODS: Employing TGF-a monoclonal antibody, signal detection was carried out by peroxidase-conjugated streptavidin in deparaffinized liver tissue sections taken from HBsAg positive patients. All of the liver tissue sections were proven HBV DNA positive by in situ hybridization. Immunohistochemical staining was performed in the tissue sections obtained from four normal controls, six from patients with chronic persistent hepatitis, five with chronic active hepatitis, eight with liver cirrhosis and eleven with hepatocellular carcinoma. RESULTS: The patterns of TGF-a immunoreactivity were cytoplasmic-grain types in normal controls and chronic persistent hepatitis, honeycomb types in chronic active hepatitis, occasional cytoplasmic-flooding types in liver cirrhosis, and cytoplasmic-grape types in hepatocellular carcinoma. A Shapiro-Wilk W test for frequency table analysis for the expression of TGF-a in these different disease groups was statistically significant. CONCLUSION: These data suggest that step-wise distinct expression of TGF-a enhancement in HBV associated chranic liver diseases which eventually resulted in the development of hepatocellular carcinoma were conceivably due to dysregulation of liver cell cycles by both HBV and TGF-a during the persistent repetition of cell cycles.
Animals
;
Carcinogenesis
;
Carcinoma, Hepatocellular*
;
Cell Cycle
;
Cell Proliferation
;
DNA
;
Hepatitis
;
Hepatitis B Surface Antigens
;
Hepatitis B virus
;
Hepatitis, Chronic*
;
Humans
;
In Situ Hybridization
;
Liver Cirrhosis*
;
Liver Diseases
;
Liver*
;
Mice
;
Natural History
;
Receptor, Epidermal Growth Factor
;
Streptavidin
8.Which of the Direct Oral Anticoagulants Is the Safest?: Based on Gastrointestinal Bleeding.
The Korean Journal of Gastroenterology 2017;69(5):328-331
No abstract available.
Anticoagulants*
;
Hemorrhage*
9.A Case of Acute Megakaryoblastic Leukemia.
Hye Sun JUNG ; Ha Baik LEE ; Hahng LEE ; Chong Moo PARK
Journal of the Korean Pediatric Society 1985;28(4):390-395
No abstract available.
Leukemia, Megakaryoblastic, Acute*
10.Species Identification of Coagulase Negative Staphylococci by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism of Heat Shock Protein 60 Gene.
Eun Jee OH ; Jung Do JANG ; Yeon Joon PARK ; Sun Moo KIM ; Byung Kee KIM
Korean Journal of Clinical Microbiology 2000;3(1):36-42
BACKGROUND: An accurate and rapid method for specise identification of coagulase negative staphylococci(CNS) has been increasingly necessary for the clinical significance and planning the management of patients with staphylococcal infections. Recently, it has been reported that there is a highly conserved area on their 60KDa heat shock protein(HSP60) gene sequences between the interspecies of CNS and it can be amplified by a set of universal degenerate primer. This led us our attention to focus on whether the PCR-based RFLP method using Mse / restriction enzyme could be a useful tool for the species identification of CNS. METHODS: In the present study, we performed PCR-based RFLP analysis using a set of degenerate primers covering HSP60 and Mse / restriction enzyme on the reference strains and 25 clinical isolates(10 of S. epidermidis, 10 of S. haemolyticus, 4 of S. lugdunensis and 1 of S. warneri) which were previously identified by the API-STAPH, Vitek GPI card and/or with conventional biochemical test. RESULT: All the seven reference strains revealed that each strain has a distinct electrophoresed band patterns with combination of different number (up to 8) and size of fragments. And these distinct band patterns showed remarkable concordance with the seven reference strains and 25 clinical isolates. CONCLUSION: These result strongly suggest that the PCR-RFLP method using degenerate primers covering the HSP60 gene and Mse / digestion enzyme offer a convenient and accurate tool for species-specific identification of CNS.
Chaperonin 60*
;
Coagulase*
;
Digestion
;
Heat-Shock Proteins*
;
Hot Temperature*
;
Humans
;
Polymerase Chain Reaction
;
Polymorphism, Restriction Fragment Length
;
Shock
;
Staphylococcal Infections