2.Immunogenicity of Aujesky's disease virus isolated from the diseased piglets in Korea I. immunogenicity of the inactivated Aujesky's disease virus with aluminum hydroxide gel adjuvant.
Moo Hyung JUN ; Hun Jun LEE ; Jeong Woo PARK ; Soo Hwan AN
Journal of the Korean Society of Virology 1991;21(2):163-172
No abstract available.
Aluminum Hydroxide*
;
Aluminum*
;
Korea*
3.Molecular Cloning and Nucleotide Sequence of the Gene Encoding Fusion(F) Protein of the Thermostable Newcastle Disease Virus Isolated from a Diseased Pheasant.
Kyung Soo CHANG ; Kui Hyun KIM ; Moo Hyung JUN ; Hee Jong SONG ; Jong Hyeon PARK
Journal of the Korean Society of Virology 1998;28(3):233-244
The gene encoding F protein of CBP-1 strain, a heat-stable Newcastle disease virus (NDV) isolated from the diseased pheasants in Korea, was characterized by reverse transcription-polymerase chain reaction (RT-PCR), nucleotide and amino acid sequences. Virus RNA was prepared from the chorioallatoic fluid infected with NDV CBP-1 virus and cDNA was amplified by RT-PCR, cloned and sequenced to analyze. The PCR was sensitive as to detect the virus titer above 25 hemagglutination unit. 1.7kb (1,707bp) size of the cDNA was amplified and cloned into BamHI site of pVL1393 Baculo transfer vector. The nucleotide sequences for F protein were determined by dye terminator cyclic sequencing using four pairs of primers, and 553 amino acid sequences were predicted. In comparison of the nucleotide sequence of F gene of CBP-1 with those of other NDV strains, the homology revealed 88.8%, 98.5% and 98.7% with Kyojungwon (KJW), Texas GB and Beaudette C strains, respectively. As the deduced 553 amino acid sequences of F protein of CBP-1 were compared with those of other NDV strains, the homology appeared 89.9%, 98.7% and 98.9% with KJW, Texas GB and Beaudette C strains, respectively. The putative protease cleavage site (112-116) was R-R-Q-K-R, indicating that CBP-1 strain is velogenic type. The amino acid sequences include 6 sites of N-asparagine-linked glycosylation and 13 cysteine residues. These data indicate that the genotype of CBP-1 strain is more closely associated with the strains of Texas GB and Beaudette C than KJW strain.
Amino Acid Sequence
;
Animals
;
Base Sequence*
;
Clone Cells
;
Cloning, Molecular*
;
Cysteine
;
DNA, Complementary
;
Genotype
;
Glycosylation
;
Hemagglutination
;
Korea
;
Newcastle disease virus*
;
Newcastle Disease*
;
Polymerase Chain Reaction
;
RNA
;
Texas
;
Viral Load
4.Detection of BLV Proviral DNA in Korean Native Goats Experimentally Infected with Bovine Leukemia Virus by Polymerase Chain Reaction.
Moo Hyung JUN ; Kyung Soo CHANG ; Young Sung CHO ; Jong Hyeon PARK ; Soo Hwan AN
Journal of the Korean Society of Virology 1997;27(2):217-226
PCR implication using the primers for gag, pol and rev genes in BLV (bovine leukemia virus) proviral DNA and syncytium assay were carried out for the Korean native goats experimentally infected with bovine leukemia virus to investigate pathogenesis of BLV in the goats, and to establish a model animal for BLV infection. The oligonucleotide primers used in PCR revealed very high specificity, The minimal amount of FLK-BLV cellular chromosomal DNA to detect the integrated BLV proviral DNA was 10 ng. The peripheral blood lymphocytes from the goat infected with BLV were examined at regular intervals by PCR amplification and syncytium assay. Pol or gag genes were detected in none of three infected goats at the 1st week post-infection (p.i.). At the 4th week p.i., one of three goats showed the amplified gag gene. Thereafter detection rates for the genes were increased, indicating that the BLV proviral genes were integrated in all of the lymphocytes from three goats, at the 16th weeks p.i., when it was evident in syncytium assay that the lymphocytes from all of three goats were infested with infective BLV. Investigating the tissues from the necropsied goats at the 8th month p.i., the amplified BLV proviral genes and infective BLV were detected in all of the peripheral lymphocytes from three infected-goats. Among various tissues examined, the amplified BLV proviral genes were observed in spleen and superficial cervical, mandibular and retropharyngeal lymph nodes, and the infective BLV, in superficial cervical and mandibular lymph nodes. It was assumed that the Korean native goat was quite susceptible to BLV infection, indicating that the goat could be a good model animal for BLV.
Animals
;
Cattle
;
Deltaretrovirus Infections
;
DNA Primers
;
DNA*
;
Enzootic Bovine Leukosis*
;
Genes, gag
;
Genes, rev
;
Giant Cells
;
Goats*
;
Leukemia
;
Leukemia Virus, Bovine*
;
Lymph Nodes
;
Lymphocytes
;
Polymerase Chain Reaction*
;
Sensitivity and Specificity
;
Spleen
5.Infectivity of bovine leukemia virus to Korean native goats I. antibody responses and syncytium assay for Korean native goats experimentally infected with bovine leukemia virus.
Young Sung CHO ; Moo Hyung JUN ; Kyung Soo CHANG ; Young Do CHOI
Journal of the Korean Society of Virology 1993;23(2):153-163
No abstract available.
Animals
;
Antibody Formation*
;
Cattle
;
Enzootic Bovine Leukosis*
;
Giant Cells*
;
Goats*
;
Leukemia Virus, Bovine*
6.Cloning and Expression of Hemagglutinin-Neuraminidase Gene of a Thermostable Isolate of Newcastle Disease Virus by Baculovirus Recombinants.
Kyung Soo CHANG ; Moo Hyung JUN
Journal of Bacteriology and Virology 2005;35(1):57-66
The hemagglutinin-neuraminidase (HN) gene of a thermostable Newcastle disease virus isolated from the diseased pheasants in Korea was cloned using Baculovirus transfer vector system, constructing pVL-NDHN inserted with HN gene (1.75 kbp). The HN recombinant baculovirus was generated in Sf-9 cells by co-transfection with pVL-NDHN and linearized baculovirus DNA. The Sf-9 cells infected with the recombinant baculovirus showed the hemagglutinating activity for chicken erythrocytes, and specific positive reactions in indirect immunofluorescence and indirect dot immunoassay. By SDS-PAGE and Western blot analysis, the expressed HN protein with the size of 74 kDa was detected in the cells infected with the recombinant baculovirus. To evaluate the immunogenicity of expressed HN protein, the chicken inoculated with the lysates of the Sf-9 cells were examined by hemagglutination inhibition and ELISA tests. The substantial levels of antibody responses were detected in both assays. The HN protein expressed in baculovirus recombinant system could be utilized for the development of diagnostic measures for Newcastle disease in poultry, and these results on HN recombinant baculovirus will expedite the development of recombinant ND vaccines.
Animals
;
Antibody Formation
;
Baculoviridae*
;
Blotting, Western
;
Chickens
;
Clone Cells*
;
Cloning, Organism*
;
DNA
;
Electrophoresis, Polyacrylamide Gel
;
Enzyme-Linked Immunosorbent Assay
;
Erythrocytes
;
Fluorescent Antibody Technique, Indirect
;
Hemagglutination
;
HN Protein
;
Immunoassay
;
Korea
;
Newcastle disease virus*
;
Newcastle Disease*
;
Poultry
;
Vaccines
7.Neonatal Giant Cell Hepatitis: An autopsy case.
Sung Churl LIM ; Moo Young SONG ; Un Jun HYUNG ; Je G CHI
Korean Journal of Pathology 1991;25(2):147-152
We report an autopsy case of neonatal giant cell hepatitis that was presumed to be related to bacterial sepsis, endotoxemia and to the subsequent parenteral alimentation and antibiotics treatment. The patient died of candidal endocarditis and multiple brain infarcts. This female baby was born by a normal full term spontaneous delivery. Six days after delivery she developed fever and lethargy as she suffered from Cheyne-Stokes respiration with severe grunting. Blood culture grew Enterobacter and Acinetobacter. After management of the sepsis her general condition improved. On the 23rd day of admission she was found to have deep jaundice and hepatosplenomegaly. The liver became larger progressively and the edge was palpable at the umbilical level. Grade II systolic murmur was heard along the left lower sternal border. She died on the 31st day of hospitalization. Postmortem examination showed severe jaundice, hepatosplenomegaly, a large vegetation on the mitral valve and multiple petechial hemorrhages of the viscera. Microscopically the liver showed features of massive giant cell transformation, mild fibrosis and inflammatory cells, suggestive of giant cell hepatitis. Numerous yeasts and candidal pseudohyphae were seen in the cardiac vegetation, focally extending into the myocardium. There was a focus of candidal vasculitis in the bowel wall. In addition there were multiple bilateral organizing infarcts in the cerebral hemisphere as well as diffuse white matter damage associated with septicemia.
Female
;
Infant, Newborn
;
Humans
8.Sequence Analysis of the Gene Encoding gp55 Protein of Suri Strain, an Attenuated Classical Swine Fever (Hog Cholera) Virus.
Kui Hyun KIM ; Kyung Soo CHANGE ; Kyong Im KANG ; Byung Hyung LEE ; Moo Hyung JUN ; Jong Hyeon PARK ; Soo Hwan AN
Journal of the Korean Society of Virology 1998;28(4):303-316
An attenuated classical swine fever virus (CSFV), Suri strain, is a va.iant derived from a vaccine virus, LOM strain. This study was performed to elucidate the molecular biologcal properties of CSFV Suri strain, and to obtain the basic data for molecular epidemiological approaches for the disease. The truncated form of gp55 gene without the C-terminal transmembrane domain, in size of 1,023bp, was amplified by RT-PCR and sequenced by dye terminator cyclic sequencing method, and inserted into BamHI site of pAcGP67B baculovirus vector, establishing a cloned pAcHEG plasmid. By the nucleotide sequences determined, 341 amino acid sequences were predicted. As compared the nucleotide and amino acid sequences of gp55 of Suri with the various CSFV, Suri strain showed the high homology over 99.1% with ALD and LOM strains, but comparably the lower homology with Alfort and Brescia. In comparison of amino acid sequence in variable domain of gp55 protein, the similar tendency of homology was observed. In hydrophobicity analysis, all of four CSFV strains revealed the analogous patterns of hydrophobicity. The numbers and locations of N-glycosylation site and cysteine residues in gp55 were analyzed, those of Suri strain being coincident with ALD and LOM strains. The results suggest that gp55 in Suri strain has the high similarity to those in ALD and LOM strains in terms of the nucleotide and amino acid sequences and the functional properties of gp55 protein..
Amino Acid Sequence
;
Animals
;
Baculoviridae
;
Base Sequence
;
Classical swine fever virus
;
Classical Swine Fever*
;
Clone Cells
;
Cysteine
;
Hydrophobic and Hydrophilic Interactions
;
Plasmids
;
Sequence Analysis*
;
Swine
9.Molecular Cloning and Nucleotide Sequence of the Gene Encoding Gp44 Protein of Suri strain: an Attenuated Classical Swine Fever Virus.
Ji Young KIM ; Kyung Soo CHANG ; Suk KIM ; Tae Yong KIM ; Kui Hyun KIM ; Jong Hyeon PARK ; Moo Hyung JUN
Journal of Bacteriology and Virology 2001;31(2):175-186
No abstract available.
Animals
;
Base Sequence*
;
Classical swine fever virus*
;
Classical Swine Fever*
;
Cloning, Molecular*
;
Swine
10.Expression of F Protein Gene of a Thermostable Isolate of Newcastle Disease Virus Using Baculovirus Expression System.
Kyung Soo CHANG ; Ji Young KIM ; Suk KIM ; Tae Yong KIM ; Hee Jong SONG ; Moo Hyung JUN
Journal of Bacteriology and Virology 2001;31(2):163-174
No abstract available.
Animals
;
Baculoviridae*
;
Newcastle disease virus*
;
Newcastle Disease*