OBJECTIVETo study the chemical constituents of the bark of Paeonia suffruticosa.

METHODThe 95% ethanol extract was re-extracted with EtOAc, and then separated and purified by column chromatography using silica gel, Sephadex LH -20 and RP-18 as packing materials. The structures were identified on the basis of spectral analysis and physico-chemical characters.

RESULTSix compounds were isolated and identified as (+)-catechin (1), paeonidanin (2), paeoniflorigenone (3), 2, 5-dihydroxy-4-methoxyacetophenone (4), paeonol (5), gallic acid (6).

CONCLUSIONThe compound 1 was isolsted from the genus Paeonia for the first time. The compounds 2, 3 were isolated from this plant for the first time.

Catechin ; chemistry ; isolation & purification ; Monoterpenes ; chemistry ; isolation & purification ; Paeonia ; chemistry ; Plant Bark ; chemistry ; Plants, Medicinal ; chemistry

A phytochemical investigation of the leaves of Rhododendron anthopogon revealed the presence of five known monoterpenes: ranhuadujuanine A (1), cannabiorcicyclolic acid (2), ranhuadujuanine B (3), ranhuadujuanine C (4) and ranhuadujuanine D (5). All compounds are firstly reported as natural products. The assignments of some 13C-signals of ranhuadujuanine A reported in the literature were revised on the basis of 2D-NMR spectra.
Biological Products ; isolation & purification ; Magnetic Resonance Spectroscopy ; Monoterpenes ; chemistry ; Plant Leaves ; chemistry ; Rhododendron ; chemistry

Seven meroterpenoids and five small-molecular precursors were isolated from Penicillium sp., an endophytic fungus from Dysosma versipellis. The structures of new compounds, 11beta-acetoxyisoaustinone (1) and isoberkedienolactone (2) were elucidated based on analysis of the spectral data, and the absolute configuration of 2 was established by TDDFT ECD calculation with satisfactory match to its experimental ECD data. Meroterpenoids originated tetraketide and pentaketide precursors, resepectively, were found to be simultaneously produced in specific fungus of Penicillium species. These compounds showed weak cytotoxicity in vitro against HCT-116, HepG2, BGC-823, NCI-H1650, and A2780 cell lines with IC 50 > 10 micromol x L(-1).
Berberidaceae ; microbiology ; Cell Line ; Cell Line, Tumor ; Humans ; Lactones ; isolation & purification ; pharmacology ; Monoterpenes ; isolation & purification ; pharmacology ; Penicillium ; chemistry

OBJECTIVETo study the chemical constituents of an active fraction (DSS-A-N-30) from Danggui Shaoyao San.

METHODDSS-A-N30 was prepared by macroporous resin chromatography, the compound was isolated by column chromatography on silica gel and RPC-18, the structure was elucidated by spectroscopic methods.

RESULTA new monoterpene glycoside was isolated and identified from DSS-A-N-30.

CONCLUSIONThe new monoterpene glycoside was identified as 4"-hydroxyl-albiflorin.

Bridged-Ring Compounds ; analysis ; isolation & purification ; Chromatography, Gel ; Drugs, Chinese Herbal ; chemistry ; Magnetic Resonance Spectroscopy ; Monoterpenes ; analysis ; isolation & purification

The column chromatography on silica gel, semi-preparative HPLC were used to separate and purify the compounds from the petroleum ether and ethanol extract of Aquilaria sinensis. Nine compounds were isolated. On the basis of their spectroscopic data, the structures were identified as 3, 3, 7-trimethyltricycloundecan-8-one (1), longifolene (2), norlongilactone (3), caryophyllenol-II (4), humulene diepoxide A (5), kobusone (6), (-)-bornyl ferulate (7), (24R) -24-ethylcholesta-4, 22-dien-3-one (8), (24R)-24-3-ono-4-en-sitosterone (9). Compounds 2-9 were isolated from this plant for the first time. compounds 1-6 are sesquiterpenes, compound 7 is a monoterpene derivative, compound 8 and 9 are steroids.
Chromatography ; methods ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Medicine, Chinese Traditional ; Monoterpenes ; chemistry ; isolation & purification ; Sesquiterpenes ; chemistry ; isolation & purification ; Thymelaeaceae ; chemistry

Six crystalline components were isolated from the lipophilic fraction of Artemisia annua L. They have been identified as four sesquiterpenes, one flavonol and one coumarin. Qinghaosu I and III are new sesquiterpenes. Five main constituents, camphene, iso-artemisia ketone, 1-camphor, β-carophyllene, and β-pinene were identified from the volatile oil of this herb.
Artemisia annua ; chemistry ; Artemisinins ; chemistry ; isolation & purification ; Bridged Bicyclo Compounds ; chemistry ; isolation & purification ; Camphor ; chemistry ; isolation & purification ; Monoterpenes ; chemistry ; isolation & purification ; Oils, Volatile ; chemistry ; Sesquiterpenes ; chemistry ; isolation & purification ; Terpenes ; chemistry ; isolation & purification

OBJECTIVETo establish a new rapid method to screen potential hepatoprotective compounds from traditional Chinese medicine, and identify the hepatoprotective compounds in Paeoniae Radix Rubra.

METHODFluorescein diacetate labelled and MTT assay were applied for screening the hepatoprotective fractions on HepG2 cells exposed to galactosamine. The active fractions were analyzed by chromatography coupled with mass spectrometry. Finally, the hepatoprotective effects of the identified compounds were validated by hepatoprotective assay.

RESULTThree hepatoprotective fractions were founded, in which three compounds were identified as paeoniflorin, ethyl palmitate and ethyl linoleate. Validation results indicated that all the three compounds can attenuate the galactosamine induced injury on HepG2 cells.

CONCLUSIONPaeoniflorin, ethyl palmitate and ethyl linoleate from paeoniae radix rubra showed potential hepatoprotective activity.

Benzoates ; isolation & purification ; pharmacology ; Bridged-Ring Compounds ; isolation & purification ; pharmacology ; Glucosides ; isolation & purification ; pharmacology ; Hep G2 Cells ; Humans ; Linoleic Acids ; isolation & purification ; pharmacology ; Liver ; drug effects ; Monoterpenes ; Paeonia ; chemistry ; Palmitic Acids ; isolation & purification ; pharmacology ; Protective Agents ; isolation & purification

OBJECTIVETo study the chemical constituents of the root of Paeonia sinjiangensis.

METHODThe constituents were isolated by silica column chromatography, and their structures were identified on the basis of spectral analysis and their physical-chemical constants.

RESULTFive compounds, paeoniflorin( I ), albiflorin (II), lactiflorin(III), daucosterol(IV), sucrose (V), were obtained.

CONCLUSIONAll of the compounds were obtained from this plant for the first time.

Benzoates ; chemistry ; isolation & purification ; Bridged-Ring Compounds ; chemistry ; isolation & purification ; Glucosides ; chemistry ; isolation & purification ; Glycosides ; chemistry ; isolation & purification ; Molecular Conformation ; Molecular Structure ; Monoterpenes ; chemistry ; isolation & purification ; Paeonia ; chemistry ; Plant Roots ; chemistry ; Plants, Medicinal ; chemistry

OBJECTIVETo prove if it is possible for using the shattering extraction with solvent to extract ingredients of traditional Chinese medicine.

METHODThe shattering extraction with solvent, the refluxing extraction and the ultrasonic extraction were used to extract paeoniflorin from Radix Paeoniae rubra, and to extract baicalein from Radix Scutellariae, and to extract chlorogenic acid from Flos lonicerae japonicae respectively, using ingredient content and extract yield as the measuring indexes.

RESULTThe content of each every ingredient obviously higher by using shattering extraction with solvent than using refluxing extraction or the ultrasonic extraction.

CONCLUSIONThe shattering extraction with solvent is a high efficiency, simple and quick extraction. It may be used to extract the ingredient of three kinds of traditional Chinese medicine.

Benzoates ; isolation & purification ; Bridged-Ring Compounds ; isolation & purification ; Chemical Fractionation ; methods ; Chlorogenic Acid ; isolation & purification ; Drugs, Chinese Herbal ; chemistry ; Flavanones ; isolation & purification ; Glucosides ; isolation & purification ; Monoterpenes ; Solvents ; chemistry ; Time Factors

OBJECTIVETo analyze the chemical components of the essential oil extracted from the seeds of Myristica fragrans (nutmeg) processed by different methods (steamed with water steam, roasted with flour, sauted with flour, roasted with talcum powder, roasted with loess, and roasted with bran) and to provide quality control foundations in the sciences.

METHODThe essential oil was extracted by steam distillation and separated with GC capillary column. The relative content of every compound was determined with area normalization method and the structures were elucidated by GC-MS technique.

RESULTFifty-eight to one hundred and four of chromatographic peaks were detected, among them seventy-six compounds accounting for 98.32% to 99.99% of the total essential oil in nutmeg were identified, which were composed of 69.15% to 97.24% for monoterpenoids and 2.06% to 25.51% for aromatic compounds of the total essential oil, respectively.

CONCLUSIONIt was shown that monoterpenoids and their derivatives were main composition, and aromatic compounds were secondary composition in the total essential oil of nutmeg grows in Indonesia and processed by different traditional methods on the basis of theory of traditional Chinese medicine. In addition, it was suggested that we should be careful to use processed nutmeg owing to contain safrole and a-asarone induced genetoxicity in animals and mutagenicity in the Ames Salmonella assay, and myristicin and elemicin induced narcotism in human. The processed method roasted with bran for nutmeg may be better and will be developed.

Anisoles ; chemistry ; isolation & purification ; Benzyl Compounds ; chemistry ; isolation & purification ; Dioxolanes ; chemistry ; isolation & purification ; Gas Chromatography-Mass Spectrometry ; methods ; Hydrocarbons, Aromatic ; chemistry ; isolation & purification ; Molecular Structure ; Monoterpenes ; chemistry ; isolation & purification ; Myristica fragrans ; chemistry ; Oils, Volatile ; chemistry ; isolation & purification ; Plant Oils ; chemistry ; isolation & purification ; Plants, Medicinal ; chemistry ; Pyrogallol ; analogs & derivatives ; chemistry ; isolation & purification ; Reproducibility of Results ; Safrole ; chemistry ; isolation & purification ; Seeds ; chemistry ; Technology, Pharmaceutical ; methods