In recent years, molecular diagnostics has been the most promising branch of
Humans ; Microfluidics ; Molecular Diagnostic Techniques/instrumentation* ; Pathology, Molecular/instrumentation*

In the post-genome era, the emphasis of the human genome project (HGP) is transferred to the study of functional genomics, which has become the hotspots of modern medicine. Studies on mechanism of various diseases could be deepened with the progressing of differential gene expression analysis technique. By taking the study on ischemic heart diseases as an example, the development of differential gene expression analysis technique and its application were reviewed.
Biomedical Research ; methods ; trends ; Expressed Sequence Tags ; Gene Expression Profiling ; methods ; Genomics ; instrumentation ; methods ; Humans ; Molecular Diagnostic Techniques ; Myocardial Ischemia ; diagnosis ; genetics ; Oligonucleotide Array Sequence Analysis

A rapid and simple method to detect tumor markers in liver cancer was established by combining immunochromatography technique with fluorescent microsphere labeling. According to the principle of double antibody sandwich, the cytoskeleton-associated protein 4 (CKAP4) paired antibody was used as the labeled and coated antibody, and the goat anti-rabbit polyclonal antibody was used as the quality control line coated antibody in the preparation of the CKAP4 fluorescent immunochromatographic test strips. After the preparation, the test strips were evaluated on various performance indicators, such as linearity, precision and stability. The CKAP4 immunochromatographic strip prepared by time-resolved fluorescent microspheres had high sensitivity, and good specificity. Its precision was within 15%, recovery between 85% and 115%, and linear range between 25 and 1 000 pg/mL. The test strip could be kept stable at 37 °C for 20 days, and it correlated well with commercial ELISA kits. The CKAP4 fluorescence immunochromatography method can quantitatively detect the content of CKAP4 in serum. Furthermore, it is rapid, sensitive, simple, economical and single-person operation. This method has the potential of becoming a new method for the diagnosis and treatment of liver cancer.
Animals ; Antibodies ; metabolism ; Chromatography, Affinity ; Fluorescence ; Humans ; Liver Neoplasms ; diagnosis ; Membrane Proteins ; isolation & purification ; Molecular Diagnostic Techniques ; instrumentation ; methods ; Sensitivity and Specificity