1.Acute and chronic exposure to Tyrophagus putrescentiae induces allergic pulmonary response in a murine model
Nailê Karine NUÑEZ ; Aline Andrea DA CUNHA ; Moisés DOS SANTOS DUTRA ; Gustavo Leivas BARBOSA ; Alessandra Loureiro MORASSUTTI ; Rodrigo Godinho DE SOUZA ; Mauro Henrique Moraes VARGAS ; Géssica Luana ANTUNES ; Josiane Silva SILVEIRA ; Guilherme Liberato DA SILVA ; Paulo Márcio PITREZ
Asia Pacific Allergy 2016;6(1):48-55
BACKGROUND: Tyrophagus putrescentiae (Tp) is a source of aeroallergen that causes allergic diseases. OBJECTIVE: To describe an acute and chronic murine model of allergic asthma with Tp extract with no systemic sensitization and no use of adjuvant. METHODS: Mites from dust sample were cultured and a raw extract was produced. Female BALB/c mice (6-8 weeks) were challenged intranasally with Tp extract or Dulbecco's phosphate-buffered saline, for 10 consecutive days (acute protocol) or for 6 weeks (chronic protocol). Twenty-four hours after the last intranasal challenge, bronchoalveolar lavage fluid (BALF) was performed for total and differential cells count, cytokine analysis, and eosinophil peroxidase activity. Lung tissue was also removed for histopathologic analysis. RESULTS: Tp extract has shown a significant increase in total cells count from BALF as well as an increase in absolute eosinophils count, eosinophil peroxidase activity, interleukin (IL)-5 and IL-13 levels, in both acute and chronic protocols. Peribronchovascular infiltrate, goblet cells hyperplasia and collagen deposition were shown in the airways of acute and chronic Tp-exposed mice. CONCLUSION: Our data suggest that the intranasal exposure to Tp extract, with no systemic sensitization and no use of adjuvants, induces a robust allergic inflammation in the lungs of mice, in both acute and chronic models. Our Tp extract seems to be a potent allergen extract which may be used in asthma model studies.
Acaridae
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Animals
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Asthma
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Bronchoalveolar Lavage Fluid
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Collagen
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Dust
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Eosinophil Peroxidase
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Eosinophils
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Female
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Goblet Cells
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Humans
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Hyperplasia
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Hypersensitivity
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Inflammation
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Interleukin-13
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Interleukins
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Lung
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Mice
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Mites
2. Fruiting increases total content of flavonoids and antiproliferative effects of Cereus jamacaru D.C. Cladodes in sarcoma 180 cells in vitro
Jean Carlos Vencioneck DUTRA ; Judá Ben-Hur DE OLIVEIRA ; Vanessa Silva DOS SANTOS ; Paula Roberta Costalonga PEREIRA ; Maria DO CARMO PIMENTEL BATITUCCI ; Jean Moisés FERREIRA
Asian Pacific Journal of Tropical Biomedicine 2019;9(2):66-72
Objective: To evaluate the influence of fruiting phenological stage on total flavonoid content, antioxidant activity, and antiproliferative effects of Cereus jamacaru (C. jamacaru) (mandacaru) cladodes and fruit. Methods: Fruit and cladodes at vegetative and fruiting stage of C. jamacaru were collected. The fruit was dissected and bark, pulp, and seeds were separated. Vegetative and fruiting cladodes, together with bark, pulp, and seeds were used to obtain five hydroalcoholic extracts. The extracts were investigated for total flavonoid content, using AlCl