1.Application of LC-MS/MS for quantitative analysis of glucocorticoids and stimulants in biological fluids
Jamshed HANEEF ; Mohammad SHAHARYAR ; Asif HUSAINA ; Mohd RASHID ; Ravinesh MISHRA ; Shama PARVEEN ; Niyaz AHMED ; Manoj PAL ; Deepak KUMAR
Journal of Pharmaceutical Analysis 2013;(5):341-348
Liquid chromatography tandem mass chromatography (LC-MS/MS) is an important hyphenated technique for quantitative analysis of drugs in biological fluids. Because of high sensitivity and selectivity, LC-MS/MS has been used for pharmacokinetic studies, metabolites identification in the plasma and urine. This manuscript gives comprehensive analytical review, focusing on chromatographic separation approaches (column packing materials, column length and mobile phase) as well as different acquisition modes (SIM, MRM) for quantitative analysis of glucocorticoids and stimulants. This review is not meant to be exhaustive but rather to provide a general overview for detection and confirmation of target drugs using LC-MS/MS and thus useful in the doping analysis, toxicological studies as well as in pharmaceutical analysis.
2.A review on therapeutic potential of Nigella sativa: A miracle herb.
Aftab AHMAD ; Asif HUSAIN ; Mohd MUJEEB ; Shah Alam KHAN ; Abul Kalam NAJMI ; Nasir Ali SIDDIQUE ; Zoheir A DAMANHOURI ; Firoz ANWAR
Asian Pacific Journal of Tropical Biomedicine 2013;3(5):337-352
Nigella sativa (N. sativa) (Family Ranunculaceae) is a widely used medicinal plant throughout the world. It is very popular in various traditional systems of medicine like Unani and Tibb, Ayurveda and Siddha. Seeds and oil have a long history of folklore usage in various systems of medicines and food. The seeds of N. sativa have been widely used in the treatment of different diseases and ailments. In Islamic literature, it is considered as one of the greatest forms of healing medicine. It has been recommended for using on regular basis in Tibb-e-Nabwi (Prophetic Medicine). It has been widely used as antihypertensive, liver tonics, diuretics, digestive, anti-diarrheal, appetite stimulant, analgesics, anti-bacterial and in skin disorders. Extensive studies on N. sativa have been carried out by various researchers and a wide spectrum of its pharmacological actions have been explored which may include antidiabetic, anticancer, immunomodulator, analgesic, antimicrobial, anti-inflammatory, spasmolytic, bronchodilator, hepato-protective, renal protective, gastro-protective, antioxidant properties, etc. Due to its miraculous power of healing, N. sativa has got the place among the top ranked evidence based herbal medicines. This is also revealed that most of the therapeutic properties of this plant are due to the presence of thymoquinone which is major bioactive component of the essential oil. The present review is an effort to provide a detailed survey of the literature on scientific researches of pharmacognostical characteristics, chemical composition and pharmacological activities of the seeds of this plant.
Humans
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Medicine, Traditional
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Nigella sativa
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chemistry
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Plant Extracts
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chemistry
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pharmacology
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therapeutic use
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Plants, Medicinal
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chemistry
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Research
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Seeds
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chemistry
3. Quantification of total phenol, flavonoid content and pharmacognostical evaluation including HPTLC fingerprinting for the standardization of Piper nigrum Linn fruits
Aftab AHMAD ; Aftab AHMAD ; Anil BHANDARI ; Asif HUSAIN ; Mohd MUJEEB ; Shah Alam KHAN ; Hani Abdullah Anber ALHADRAMI
Asian Pacific Journal of Tropical Biomedicine 2015;5(2):101-107
Objective: To carry out the physicochemical and phytochemical standardization with high performance thin layer chromatography fingerprinting of Piper nigrum L. (P. nigrum) fruits in order to ascertain the standard pharmacognostical parameters of this king of spices. Methods: Many standardization parameters like extractive values, total ash value, water soluble ash value and acid insoluble ash, moisture content, loss on drying and pH values of P. nigrum L. fruits were analyzed. The method of Harborne was adopted for the preliminary phytochemicals screening. Analysis of total phenolic and flavonoid contents, pesticides residues, aflatoxin and heavy metals were also performed. CAMAG-high performance thin layer chromatography system was used for fingerprinting of methanolic extract of P. nigrum L. fruits. Results: The results of phytochemicals testing indicated the presence of carbohydrates, phenolic compounds, flavonoids, alkaloids, proteins, saponins, lipids, sterols and tannins in various solvent extracts. Total phenolic and flavonoid contents in methanolic extract were found to be 1.728 1 mg/g and 1.087 μg/g, respectively. Heavy metals concentrations were found to be within standard limits. Aflatoxins and pesticides residues were absent. Conclusions: The outcome of this study might prove beneficial in herbal industries for identification, purification and standardization of P. nigrum L. fruits.
4.New insights on the utilization of ultrasonicated mustard seed cake: chemical composition and antagonistic potential for root-knot nematode,
Hera NADEEM ; Pieter MALAN ; Amir KHAN ; Mohd ASIF ; Mansoor AHMAD SIDDIQUI ; Simon TUHAFENI ANGOMBE ; Faheem AHMAD
Journal of Zhejiang University. Science. B 2021;22(7):563-574
This study focused, for the first time, on the effect of ultrasonic features on the extraction efficiency of secondary metabolites in mustard seed cake (MSC). The nematostatic potential of sonicated seed cake was examined against the second-stage juveniles (J2s) of root-knot nematode,