1.Prevalence of Enteric Protozoan Oocysts with Special Reference to Sarcocystis cruzi among Fecal Samples of Diarrheic Immunodeficient Patients in Iran.
Mahmoud AGHOLI ; Shahrbanou Naderi SHAHABADI ; Mohammad Hossein MOTAZEDIAN ; Gholam Reza HATAM
The Korean Journal of Parasitology 2016;54(3):339-344
The genus Sarcocystis is not usually considered as an important enteric pathogen in immune compromised patients. It might be expected that species for which humans are the final host (Sarcocystis hominis and Sarcocystis suihominis as well as possibly others) would be encountered increasingly often in immunodeficient persons. This study aimed to address how to detect and differentiate Sarcocystis oocysts and/or sporocysts from enteric protozoans in the diarrheal samples of immunodeficient patients in Shiraz, Iran. Diarrheal samples of 741 immunodeficient patients with recurrent persistent or chronic diarrhea were examined by microscopy and molecular biological analysis. Oocysts-positive samples were 68 Cryptosporidium spp., 9 Cystoisospora belli (syn. Isospora belli), 2 Cyclospora cayetanensis, and 15 microsporidia (Enterocytozoon bieneusi). Sarcocystis-like sporocysts found from a woman were identified as Sarcocystis cruzi through 18S rDNA amplification and phylogenetic analysis. To the best of our knowledge, this is the first report of S. cruzi from a human.
Cryptosporidium
;
Cyclospora
;
Diarrhea
;
DNA, Ribosomal
;
Female
;
Humans
;
Iran*
;
Isospora
;
Microscopy
;
Microsporidia
;
Oocysts*
;
Polymerase Chain Reaction
;
Prevalence*
;
Sarcocystis*
2.Anthropometric Indices from Primary to High School in the West of Iran: Epidemiologic Trends.
Badrieh SAHARGAHI ; Hadi ABDOLLAHZAD ; Jalal MOLUDI ; Seyed Mostafa NACHVAK ; Yahya PASDAR ; Mohammad Reza NADERI ; Mansour REZAEI
Clinical Nutrition Research 2018;7(3):189-198
Examining the trend of anthropometric indices in children and adolescents in each region can be highly beneficial in providing effective strategies to improve the status of their growth. This study was conducted with the aim of determining the trend of anthropometric indices in students from primary to high school in the west of Iran. Data were collected using a demographic questionnaire and health certificates for anthropometric information of primary school students, and current anthropometric measurements with scale for high school students. Then, all of the data were analyzed in AnthroPlus and SPSS software. Of the 731 students, 350 were female and 381 were male. Mean height Z-score to age showed a significant reduction from primary to high school (from +0.0386 to −0.27416), and mean body mass index Z-score to age showed a significant increase (from −0.3916 to +0.1826). Prevalence of stunting and overweight/obesity in high school was 1.4 and 2.5 times higher than primary school, respectively, but the prevalence of wasting reduced by 2.3 fold. Girls were more affected by the increased trend of obesity and stunting compared to boys. Nutritional transition was evident in students. Healthcare policy-makers should design and implement a comprehensive health strategy to deal with this situation, especially in girls.
Adolescent
;
Anthropometry
;
Body Mass Index
;
Child
;
Delivery of Health Care
;
Female
;
Growth Disorders
;
Humans
;
Iran*
;
Male
;
Obesity
;
Prevalence
3.Chemokine Receptors Expression in MSCs: Comparative Analysis in Different Sources and Passages.
Asieh HEIRANI-TABASI ; Shirin TOOSI ; Mahdi MIRAHMADI ; Mohammad Amir MISHAN ; Hamid Reza BIDKHORI ; Ahmad Reza BAHRAMI ; Javad BEHRAVAN ; Hojjat NADERI-MESHKIN
Tissue Engineering and Regenerative Medicine 2017;14(5):605-615
MSC-based therapy is providing a cure for degenerative diseases with unmet medical need and usually iliac crest bone marrow (ICBM) are being applied in clinics. Alternative sources, including adipose tissue and reamer/irrigator/ aspirator hold great potential for isolating MCSs. Here, we compared original MSCs features of adipose tissue (Ad-MSCs) and bone marrow of long-bone (RIA-MSCs) or iliac crest, and the expression of chemokine receptors (including CXCR4, CX3CR1, CXCR6, CXCR2, CCR1 and CCR7) in these three sources, which are important in the context of homing. We further investigated the role of SDF-1/CXCR4 axis as a key player in motility of different population of MSCs using Transwell migration assay. All cells exhibited typical MSCs characteristics. However, different MSCs sources expressed different levels of chemokine receptors. Generally, the expression of these chemokine receptors was decreased with increasing passage (P) number from 2 to 3. Interestingly, it was observed that the CXCR4 expression and migration capacity in Ad-MSCs is significantly higher than ICBM and RIA-MSCs in P2. Although our data showed that CXCR4 had highest expression in P2 Ad-MSCs, but it dramatically declined following sub-culturing in the P3. Hence, to improve homing of MSCs by means of chemokine/their receptors axis, the source of isolation and passage number should be considered for clinical applications.
Adipose Tissue
;
Bone Marrow
;
Receptors, Chemokine*
;
Stem Cells