1.The inhibitory effects on adult male reproductive functions of crude garlic (Allium sativum) feeding.
Imen HAMMAMI ; Afef NAHDI ; Claire MAUDUIT ; Mohamed BENAHMED ; Mohamed AMRI ; Awatef Ben AMAR ; Semy ZEKRI ; Ahmed El MAY ; Michele Veronique El MAY
Asian Journal of Andrology 2008;10(4):593-601
AIMto investigate the effects of crude garlic on adult male rat reproductive functions.
METHODSThirty male rats were divided into five groups: group 1 (untreated) and groups 2, 3, 4 and 5 were fed for 30 days with 5%, 10%, 15% and 30% crude garlic, respectively. Testes and accessory organs were weighed and some markers were assessed. Light and electron microscopy observations were also performed.
RESULTSA significant decrease was observed in the body weight of groups 4 (14%; P < 0.01) and 5 (20%; P < 0.01); of the prostate weight in group 5 (29.1%; P < 0.05) and of seminal vesicle weight in groups 3 (14.4%; P < 0.01), 4 (18.3%; P < 0.01) and 5 (27.3%; P < 0.01). In contrast, testis and epididymis weights were unchanged. In epididymis tissue, the alpha glucosidase activity and the spermatozoa density were unchanged. The treatment resulted in a significant decrease in testosterone serum levels in groups 3 (77.3%; P < 0.01), 4 (77.3%; P < 0.01) and 5 (90.9%; P < 0.01), associated with a significant increase in LH serum levels (P < 0.01). Testicular histology showed a dose-dependent increase in the percentage of empty seminiferous tubules. Moreover, testicular function was affected; a significant decrease in phosphatase acid activity (P < 0.01) and testosterone (P < 0.05) contents were observed.
CONCLUSIONCrude garlic consumption during 1 month reduced testosterone secretion and altered spermatogenesis at 10%, 15% and 30% doses.
Animals ; Dose-Response Relationship, Drug ; Epididymis ; drug effects ; physiology ; Garlic ; adverse effects ; Leydig Cells ; drug effects ; physiology ; Luteinizing Hormone ; blood ; Male ; Plant Preparations ; pharmacology ; Prostate ; drug effects ; physiology ; Rats ; Rats, Wistar ; Reproduction ; drug effects ; physiology ; Seminal Vesicles ; drug effects ; physiology ; Sertoli Cells ; drug effects ; physiology ; Sperm Count ; Spermatogenesis ; drug effects ; physiology ; Testis ; cytology ; drug effects ; metabolism ; Testosterone ; blood
2.Differential Alterations of Lipid Status and Lipid Metabolism, Induction of Oxidative Stress by Sodium Arsenate in Female Rat's Liver and Kidney.
Wafa KHARROUBI ; Madiha DHIBI ; Imed CHREIF ; Lizard GÉRARD ; Mohamed HAMMAMI ; Rachid SAKLY
Biomedical and Environmental Sciences 2015;28(11):829-833
Animals
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Arsenates
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toxicity
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Female
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Kidney
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drug effects
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Lipid Metabolism
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drug effects
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Liver
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drug effects
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Oxidative Stress
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drug effects
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Rats, Wistar
3.Antioxidant and antihyperlipidemic activity of Costus speciosus against atherogenic diet-induced hyperlipidemia in rabbits.
Fatma Mohamed Hussein SHEDIWAH ; Khalid Mohammed NAJI ; Hussein Saleh GUMAIH ; Fatima A ALHADI ; Amani Lutf AL-HAMMAMI ; Myrene Roselyn D'SOUZA
Journal of Integrative Medicine 2019;17(3):181-191
OBJECTIVE:
An extract of Costus speciosus (CSE), a herb widely used in folk medicine, was evaluated for its antioxidant, antihyperlipidemic and ameliorating effects on histopathological changes in atherogenic rabbits.
METHODS:
Twenty-four male rabbits (Oryctolagus cuniculus) were divided into 4 groups. Three groups were fed a diet containing 3% saturated fat and 1.3% cholesterol for 40 d. One of these was sacrificed on the 40th day and was called the pathogenic (P) group; the other two groups received treatment for another 30 d as follows: one received 0.8 g/(kg·d) of CSE and the other was given 0.01 g/(kg·d) of simvastatin. The normal group was sacrificed on the 70th day and used as a control.
RESULTS:
CSE showed radical-scavenging ability. Administration of CSE for a 30-day period resulted in a significant decrease in total cholesterol, triacylglycerol, low-density lipoprotein and aspartate aminotransferase compared to the P group, while levels of hemoglobin, packed corpuscular volume and red blood cells were elevated. With respect to studies performed on the heart, a decrease in malondialdehyde and an increase in reduced glutathione were noted. Total protein increased in the liver, heart and aorta after treatment with CSE and also a marked improvement in histopathological parameters was demonstrated.
CONCLUSION
The present findings indicate that the C. speciosus rhizome possesses antiatherogenic and antioxidant properties which may provide protective effects against oxidative stress in atherosclerotic rabbits.