The sample introduction system of a cool vapor atomic fluorescence spectrometer made domestically was modified for taking the mercury vapor which was pre-concentrated in a capture mercury gold cartridge as gold amalgam generated on-line by pyrolyzing atmospheric particulate collected from an air conditioner. The temperatures that applied) to sample pyrolyzer to generate elemental mercury and capture mercury gold cartridge to release mercury from amalgam were 700-750 ℃ and 550 ℃, respectively. The carrier Ar flow rate was 120 mL/min. The method detection limit was 1 pg, mercury content showed a linear relationship in 0-1.5 ng), and RSD was 2.2%. The method was validated by determining mercury in the national soil standards) GBW07410. The method was Applied to determine the mercury content in the atmospheric particulate from a hospital air conditioner.

Objective To summarize the theoretic evidence of Shenmen (HT7) Point in the treatment of insomnia and its action mechanism, for guiding clinical application. Method The reasons and evidences for using acupuncture at Shenmen to treat insomnia were summarized from the following 3 aspects: records in the ancient literatures, clinical application, and modern medical studies. Result Acupuncture at Shenmen can produce a calming and sedative effect, and it should be one of the optimal choices for treating insomnia due to its significant efficacy; it calms the mind and improves sleep quality via activating the nervous center for regulating sleep and promoting the secretion of neurotransmitters such as 5-hydroxytryptamine (5-HT) in brain. Conclusion Acupuncture at Shenmen is an effective method is treating insomnia.

Objective To investigate the clinic effect of trans-artery intrahepatic transplantation of porcine islet in type 1 diabetic patients. Methods Four patients with type 1 diabetes were perfused newborn porcine islets through the hepatic artery.Fasting blood glucose(FBS), postprandial blood glucose (PBS), HBA1,dose of insulin and function of liver and kidney were measured before and after the transplantation.Results The levels of FBS,PBS and HBA1 were 7 4～13 5mmol/L,7 8～18 6mmol/L and 7 8～12 0mmol/L respectively before the transplantation. During one and half years after transplantation, the levels of FBS,PBS and HBA1 were 3 3～7 1mmol/L,6 0～8 8mmol/L and 6 4～7 6mmol/L respectively with notably reduced dosage of insulin(32～58%).The function of liver and kidney of the patients remained normal.Conclusions Trans-artery intrahepatic transplantation of porcine islet is effective and safe for type 1diabetic treatment.

Objective To investigate the effect of the serum ferritin,folic acid and its clinical efficacy by Chinese angelica Astragalus decoction combined with Iron Sucrose Injection in the treatment of the anemia patients with chronic renal failure.Methods 66 anemia patients with chronic renal failure were selected and randomly divided into the control group and the experiment group with 33 cases in each group.The control group were treated by Venofer and the experiment group were treated on the base of the control group with Chinese angelica and Astragalus Decoction.The clinical curative effect before and after treatment,serum ferritin,folic acid(SF),serum creatinine(Scr),urea nitrogen(BUN),creatinine clearance rate(Ccr),blood uric acid(UA),cholesterol(TC),total cholesterol(TG),high density lipoprotein(HDL-C)were compared after 6 months of treatment and the adverse reaction conditions was compared during the treatment between two groups.Results The effective rate of treatment in the control group was 69.70％lower than that in the observation group,the effective rate was 90.91％,with statistical significance(P<0.05).Compared with the control group,the SF and folic acid levels were lower,the Scr and BUN levels were lower,the Ccr and UA levels were higher,the TC and TG levelswere lower,the HDL-C level was higher,with statistical significance(P<0.05).Conclusion Angelica Astragalus decoction combined with Iron Sucrose Injection in the treatment of chronic renal failure patients with anemia effect is significant,can effectively reduce the level of serum ferritin and folic acid,the clinical effect is better.

Objective To study the expression of a novel inhibitor of apptosis and survivin in cervical carcinoma and its relationship to the expression of Bcl-2.Methods Using SP immunohistochemical technique, we examined the expression of survivin and Bcl-2 in 59 cervical invasive squamous cell carcinomas.Results Survivin was expressed in 41 of 59 cases(69.5%) of cervical carcinomas. In contrast, no expression of survivin in normal cervical tissues was observed. Overexpression of survivin was related to the tumor grade and clinical stage. Survivin positive cases were strongly associated with Bcl-2 expression(80% versus 35.7%;P＜0.005).Conclusion Apoptosis inhibition by survivin abnormal expression, alone or in cooperation with Bcl-2, may participate in the onset and progression of cervical carcinoma. Survivin is a new diagnostic/therapeutic target in cervical cancer.

Acupuncture Therapy ; Adult ; Aged ; Female ; Foot Diseases ; etiology ; physiopathology ; therapy ; Humans ; Intervertebral Disc Displacement ; complications ; surgery ; Male ; Middle Aged ; Paralysis ; etiology ; physiopathology ; therapy ; Young Adult

Objective To study the clinical manifestations and influencing prognostic factors of children with acute paraquat in-toxication ,and to search for reasonable and effective treatments .Methods Retrospectively analyzed the clinical data of 68 children with acute paraquat intoxication from March 2005 to June 2012 .According to the amount of toxic dose and clinical symptoms ,all of them were divided into 3 groups ,mild type(22 cases) ,severe type(26 cases) and fulminant type(14 cases) ,retrospective analysis on clinical manifestation ,auxiliary examination and prognosis among the groups and following up the survival of these patients ,the risk factors which may affect the prognosis were explored .Results 66 cases were poisoned by taking orally and the other 2 cases were poisoned by the skin absorption .Gastrointestinal symptoms were the most common .Among 68 cases ,6 cases was lost the following up ,20 cases died(32 .26% mortality):all children from mild type survived ;8 patients from severe type and 12 patients from fulmi-nant type(30 .77% ,85 .71% ,mortality respectively ) .As the degree of the clinical classification deteriorated ,the mortality in-creased ,there was statistical signification between each other and so did the blood urea nitrogen and serum creatinine (P<0 .05) .By following up ,children who once passed the acute stage can recovery and the pulmonary fibrosis might be reversed by the chest CT follow-up .The logistic regression analysis indicated that children who had been diagnosed with acute kidney injury and multiple or-gan dysfunction syndrome showed the worse prognosis .Conclusion Acute kidney injury is a very important factor for the short term prognosis of paraquat intoxication ,while pulmonary fibrosis determine the long-term outcomes ,lung injury may be reversed in the course of the disease ,so we should take active treatments .

Objective To know the position and shape of implanted electrode array with plain X-ray after cochlear implantation. Methods According to the Cochlear View developed by Xu plain X-ray was taken postoperatively to obtain the best view of electrode array. Results The Medical imaging of plain X- ray taken before switching on or following the concerning of the performance could assist in the detection of electrode arrays in improper position, partial insertion or bending inside the cochlea. It helped audiologists to make decisions to switch off affected electrodes for the optimization of the program, if functioning electrodes were insufficient and recipients failed. Conclusion It is recommended to conduct post-operative X-ray examination routinely for cochlear implant recipients. Audiologists should be able to use medical imaging to identify the position of intra-cochlear electrodes and apply the information to guide the mapping work.

Objective Our purpose was to observe the renal pathological changes in the mouse modells of adriamy -cin-induced nephropathy in different periods .Method 48 healthy male BALB/c mice were randomly divided into control group and model group .The model group received a disposable tail vein injection of adriamycin 10.5 mg/kg body weight , and the control group received the same amount of saline .24-hour urinary protein , serum biochemical indexes and kidney pathological changes were dynamically observed for 12 weeks.Results Proteinuria of model mice appeared in the 2th week after ADR injection, which lasted to the end of the 12-week experiment, At the 8th week, the amount of urine protein reached a peak (P<0.05);The serum albumin was decreased at the 4th week, cholesterol was increased at 8th week.At the end of experiment, serum creatinine was also increased (P<0.05).Minimal change nephrotic syndrome (MCNS) was observed in model mice at the 4th week;the lesions in renal tissues at 8th weeks were more serious than that at 4th weeks, but glomerular sclerosis was unconspicuous .Focal segmental glomerulonephritis ( FSGS) was seen at the 12th week.The GSI of the model mice was(2.81 ±0.84)%, significantly higher than that of the control mice ((0.33 ±0.21)%) at 12th week(P<0.01).Conclusions A mouse model with adriamycin-induced-nephrosis can be successfully established by a disposable tail vein injection of adriamycin in a dose of 10.5 mg/kg body weight .The early manifest ation of this model is MCNS, and at a late stage , it may be changed into FSGS .