Objective: To investigate the effects of gene silencing and overexpression of RACK 1 (receptor for activated C kinase 1) on the proliferation of large-cell lung cancer H460 cells and lung adenocarcinoma A549 cells, and to explore the possible mechanism. Methods: The RACK1 siRNA (small interfering RNA) targeting RACK 1 gene and recombinant vector pCMV-sport6-RACK1 were transfected into both of H460 cells and A549 cells, respectively. MTT method and colony formation assay were used to detect the effect of RACK 1 gene expression on the proliferation of lung cancer cells. Flow cytometry was used to examine the change of cell cycle. The association and interaction of RACK 1 gene expression with the proliferation of lung cancer cells were analyzed by yeast two-hybrid system, co-immunoprecipitation, laser scanning confocal microscopy and co-immunoprecipitation of phosphoproteins. Results: The expression levels of RACK1 protein in the H460 cells and A549 cells were both decreased after transfection with RACK1 siRNA, and the abilities of proliferation and colony-formation were also weakened. The proportion of lung cancer cells arrested at phase S was significantly declined (P <0.01). Meanwhile, the expression level of RACK1 protein was increased after transfection with pCMV-sport6-RACK1, and the abilities of proliferation and colony-formation of lung cancer cells were both strengthened with a prolonged doubling time. The proportion of lung cancer cells arrested at phase S was significantly increased (P <0.01). The results of yeast two-hybrid system and co-immunoprecipitation revealed that RACK1 could directly interact with MCM7 (minichromosome maintenance protein 7). The phosphorylation of MCM7 protein was strengthened through binding to RACK1 which translocated into the cell nucleus. Conclusion: RACK1 promotes the proliferation of lung cancer cells through activating the phosphorylation of MCM7 binding to RACK1. Copyright© 2012 by TUMOR.

Objective To study the technology of multi-parameter monitoring system based on ZigBee wireless network.Methods A asynchronous serial interface of IC CC2430 was used to receive the data of multi-parameter patient monitor,and then the ZigBee channels of IC CC2430 was sent to transmit the data to the central monitoring control system.Results The data of eight multi-parameter patient monitors was successfully transmitted to the central control system,and was not interfered by wireless transmission circuits.Conclusion ZigBee wireless network technology can be successfully applied to a wireless network of multi-parameter patient monitor control system.

Rhinitis,sinusitis,nasal polyps and bronchial asthma are common respiratory diseases,and they have some similarities in etiology,pathogenesis,histopathology and pathophysiology.Many patients suffer from these diseases in succession,or at the same time.Many domestic and international clinical and fundamental studies have aroused widespread concern about the relevance of these diseases,with the correlations between nasal polyps-sinusitis and asthma that attribute to much attention.However,the relevance of extent on the study of these diseases,as well as diagnosis,treatment,and concept is not very clear yet.The main target of this article is to discuss the above issues and remind our clinicians to pay more attention to understand the comprehensive relationships of rhinitis,sinusitis,nasal polyps and bronchial asthma,and provide references for the strategy of rational and effective treatment options.

OBJECTIVE: A im To investigate the role of qercetin(Que) -induced autophagy in the death of hepatocellular carcinoma cell line SMMC-7721 cells. METHODS: After treatment with different concentration of Que, the growth inhibition of the SMMC-7721 cells were assessed by MTT colorimetric assay. The fluorescent staining was applied to identify the autophagy after Que treatment. Lactate dehydrogenase (LDH) leakage and Western blot analysis were used to study the autophagic mechanisms involved in death of SMMC-7721 cells. RESULTS: The proliferation of SMMC-7721 cells were significantly inhibited in a dose and time-dependent manner after Que treatment. Autophagy was also induced in MCF-7 cells as detected by MDC staining and Fluorescent staining in the early phase. The autophagy specific inhibitor 3-MA or chloroquine potentiated Que's cytotoxicitiy in SMMC-7721 cells when administered 1h before Que; The expression of cathepsin B increased after Que treatment. CONCLUSION: Que can significantly inhibit the growth of the SMMC-7721 cells by inducing the autophagy, which is a protection mechanism that can reduce the cytotoxicity induced by Que in SMMC-7721 cells. The activation of Cathepsin B is considerable in autophagy process.

Objective To investigate the clinical effects of retroperitoneal laparoscopic surgery in the management of diseases of adrenal glands, kidneys and ureters. Methods By using a closed puncture into retroperitoneal space to establish a surgical access ,87 patients with diseases of adrenal glands, renal cysts, ureteral calculi and ureteral deformities were operated on by means of retroperitoneal laparoscopic surgery. Results The surgery was successfully accomplished in 83 patients with an excellent therapeutic effect, whereas a conversion to open surgery was required in 4 patients. There were no blood transfusions or serious complications in the study. Conclusions Retroperitoneal laparoscopic operations of adrenalectomy, cyst unroofing, ureterolithotomy, ureterolysis and ureteroplasty has the advantages of minimal invasion, less blood loss and fewer complications, being an effective and practical procedure .

ObjectiveTo search for the optimum external masking time and tonal models for patients with tinnitus.MethodsTwenty-seven patients with cochlear tinnitus were selected respectively from three different out-patients departments and divided into three groups. Nine patients in every group were treated with different masking parameters according to factorial design which had three masking noises, i.e. narrow band noise (NBN), wide band noise (WBN), speech noise (SN),and three masking time ranges, i.e. 15,30,45 minutes. The results before and after treatment were compared and analysed with analysis of variance (ANOVA). ResultsThe treatment effect when masking for 15 minutes was worse than that for 30,45 minutes, but there was no difference between 30 and 45 minutes. The NBN had the best effect when tinnitus signal was single, the WBN was effective for tinnitus with multiple sounds. ConclusionNBN or WBN masking for 30 minutes showed better therapeutic effect.

There exist many studies on the radioligands for imaging of nicotinic acetylcholine receptors (nAChRs) in the human brain and the most common one is the radioligand binding to α4β2-nAChRs,the main subtype of cerebral nAChRs.There are few data published in the literature on the radioligands for the imaging of α7-nAChRs,another important subtype of cerebral nAChRs.This review summarizes recent work on PET radioligands for the imaging of cerebral nAChRs.

The sample introduction system of a cool vapor atomic fluorescence spectrometer made domestically was modified for taking the mercury vapor which was pre-concentrated in a capture mercury gold cartridge as gold amalgam generated on-line by pyrolyzing atmospheric particulate collected from an air conditioner. The temperatures that applied) to sample pyrolyzer to generate elemental mercury and capture mercury gold cartridge to release mercury from amalgam were 700-750 ℃ and 550 ℃, respectively. The carrier Ar flow rate was 120 mL/min. The method detection limit was 1 pg, mercury content showed a linear relationship in 0-1.5 ng), and RSD was 2.2%. The method was validated by determining mercury in the national soil standards) GBW07410. The method was Applied to determine the mercury content in the atmospheric particulate from a hospital air conditioner.

Objective To discuss oral nursing frequency of patients undergoing orally mechanical ventilation in order to ensure the oral cleanness of patients with the least oral nursing frequency and prevent the occurrence of ventilator associated pneumonia (VAP). Methods 36 patients undergoing orally me-chanical ventilation were randomly divided into groupA, B, C and D with 9 cases in each group, which all adopted 0.5% of pevidone iodine solution for oral nursing, time intervals of oral nursing were 4 hours, 6 hours, 8 hours and 12 hours. After the oral care and oral care before the next oropharyngeal samples for se-mi-quantitative bacterial colony counts, bacterial strain identification were obtained. The incidence rate of VAP was also compared and the results underwent χ2 test. Results Significant difference was seen in bacterial colony counts, especially during the time interval of 6 hours and 12 hours. No significant differ-ence was seen in incidence of VAP between the 4 groups. Conclusions The desirable frequency of oral nursing was every 6 hours on the premise of prevention of VAP.