Objective To understand the implementation status and effect of prevention and control of malaria in Libo Coun?ty so as to provide the evidence for improving the malaria elimination working. Methods The data about malaria from the county CDC and county hospital were collected and 16 villages from 8 townships were randomly sampled and 10 villagers of each village were investigated. Other information about the prevention and control of malaria was also investigated. Results The inci?dence of malaria was decreasing annually from 5.75 per 10 000 in 2008 to zero in 2012. The malaria monitoring could be well conducted in the county and township levels. The infection source could be controlled in time. The utilization rate of anti?mosqui?to facilities in the residents was 93.25%and the awareness rate of knowledge about malaria prevention and control was 40.13%. Conclusions The implementation and effect of prevention and control of malaria are satisfactory in Libo County but the medi?um control is limited and the active protection consciousness of the residents is not strong. Therefore the task of malaria elimina?tion is still very arduous.

The development of DNA chip has been strongly driven by modern approaches of life science and microelectronics. The broad of DNA chip applications include the detection of pathogens,the detection of mutations in genes involved in human diseases or affected during cancer progression, sequence analysis, the pattern of gene expression monitoring and pharmacy studies .This review explains the technology, its scope, and impact on medicine, as well as its cost and possible limitations.

Objective To introduce the clinical application of the improved interosseous dorsal antidromic artery issular flap in the emergency treatment of the soft tissue defects in hand trauma. Methods 16 patients were received the interosseous dorsal antidromic artery flap for soft tissue defects in the hand with emergency treatment. Results All 14 flaps survived, tip necrosis occurred in 2 cases which healed without complications. Conclusion It was simple technical and shorten time in hospital with improvement of the emergency operative procedure of interosseous dorsal antidromic artery flap, and could reduce the impact of frequency of anatomic variations and vascular complications.

Objective To establish a method for the determination of Paracetamol in Zihua Ganmao Capsule. Method Samples were separated on a Kromasil-C18 column with the mobile phase of methanol- water (35∶65), and the detection wavelength was set at 249 nm. Result The calibration curves were linear in the range of 0.165～0.99 ?g (r=0.999 9) for Paracetamol. The average recovery was 98.41% and RSD= 0.64% (n =6). Conclusion The method is accurate and repeatable, and can be used for the quality control of Zihua Ganmao Capsule.

Aim To investigate the antagonistic action o f total saponins of panaxnotoginseng(PNS) on cardiac hypertrophy and its nervous mechanism.Methods (1)cardiac hypertrophy of rats due to pressure overload was induced by constricting of abdominal aorta. There were five groups in the experiments. The rats in Group A(control group)were sham operated . Group B was aorta-constricted group.The rats in Group C,D,E were given ip PNS 50,100,150 mg?kg?d -1 for three weeks respectively. Three weeks later, We measured the heart-weight(HW),left ventricular weight(LVW), the ratio of HW/BW,LVW/BW (LVI) and the cardiomyocyte diameters(MD) after dyeing by HE color.(2)The effects of PNS on the fast excitatory postsynaptic potential(f-EPSP),membrane depolarization induced by application of acetylcholine (ACh),membrane potential and membrane resistance of the isolated Stellate ganglion(SG)of the rats were investigated by means of intracellular recording techniques. Results (1)HW/BW, LVI and MD of Group E were significantly lower than that of Group B(P0.05).(2)At the concentration of 0.10 ～0.16 g?L -1, PNS reversibly depressed the amplitude of f-EPSP, but the ACh depolarization,membrane potential and membrane resistance were not significantly altered by PNS. Conclusion PNS can prevent cardiac hypertrophy due to pressure overload in rats and this action may underline its inhibitory action on presyn aptic effect of regulating calcium influx.

Aim To study the effect of rhynchophylline on NMDA receptor 2B subunit(NR2B) mRNA expression in the nucleus accumbens(NAc) and amygdala(Amy) of amphetamine-induced condictioned place preference(CPP) rats.Method 56 male SPF grade Sprague-Dawley rats were randomly divided into control group,amphetamine-induced model group,model group with ketamine,model group with low,middle,high dose of rhynchophylline,and control group with rhynchophylline(n=8 for each group) The time of rats' staying in white compartment was measured in the 5th day,then NR2B mRNA expression in NAc and Amy of each rat was detected with in situ hybridization technology.Results The rats exhibited CPP after treated with 2 mg?kg-1?d-1 amphetamine for 4 d.Ketamine and rhynchophylline eliminated CPP effect,and the effect of rhychophylline showed a dose-dependence.In amphetamine-induced model group,NR2B mRNA expression in NAc and AMY of rats was significantly increased.Ketamine,middle and high dose of rhynchophylline blocked the increase of NR2B mRNA expression induced by amphetamine.Rhynchophylline had no effect on the basal level of RN2B mRNA expression in normal rats.Conclusion Inhibition of NR2B mRNA expression in NAc and AMY may contribute to the antagonistic effect of rhychophylline on amphetamine-induced CPP.

ObjectiveTo investigate the association between endothelial NO synthase (eNOS) gene G10T polymorphism and the susceptibility of sporadic congenital heart disease (CHD).MethodsThe genotype oneNOS G10T locus was detected and compared in 1323 children with sporadic CHD and 1323 non-CHD children.ResultsCompared with the CC genotype, the AA genotype signiifcantly increased the risk of CHD (adjustedOR=1.42, 95%CI=1.01-2.04). Compared with the CC/AC geno-type, the AA genotype signiifcantly increased the risk of CHD (adjustedOR=1.39, 95%CI=1.08-1.92). Based on stratiifed analy-sis, the AA genotype was associated with the susceptibility of perimembranous ventricular septal defects (adjustedOR=1.56, 95%CI=1.17-2.47).ConclusionsIn Chinese population, theeNOS G10T polymorphism may increase the susceptibility of sporadic CHD.

Gastroparesis is a clinical syndrome characterized by delayed gastric emptying of meal in the absence of mechanical obstruction of gastric outlet. In this article,the pathogenesis,etiology,epidemiology,clinical features, diagnosis and treatment of gastroparesis were reviewed.

Objective To study the security and effectiveness of leg lengthening in increasing the height of short stature for cosmetic reasons. Methods The improved bone lengthening technique was used to 60 adult short stature volunteers to prolong bilateral lower legs. All data, including healing time of the distracted bone, operative complications, recovery of limb function and height increment, were recorded. Results In all cases, the height increment was 5 to 10 cm (mean 6.8cm). Bone lengthening rate was 19.6 %. The healing time of the distracted bone was 6 to 16 months (mean 8.2 months). There was no severe complication occurred. Follow up for 2 to 8 years showed that all cases returned to work or study with normal gaits and satisfied body figure. Previous psychologic obstacles were eliminated. Two years after bone healing, the ability for intense activities was regained. Conclusion Leg lengthening for cosmetic heightening is a safe and effective therapeutic approach.