The development of DNA chip has been strongly driven by modern approaches of life science and microelectronics. The broad of DNA chip applications include the detection of pathogens,the detection of mutations in genes involved in human diseases or affected during cancer progression, sequence analysis, the pattern of gene expression monitoring and pharmacy studies .This review explains the technology, its scope, and impact on medicine, as well as its cost and possible limitations.

Objective To introduce the clinical application of the improved interosseous dorsal antidromic artery issular flap in the emergency treatment of the soft tissue defects in hand trauma. Methods 16 patients were received the interosseous dorsal antidromic artery flap for soft tissue defects in the hand with emergency treatment. Results All 14 flaps survived, tip necrosis occurred in 2 cases which healed without complications. Conclusion It was simple technical and shorten time in hospital with improvement of the emergency operative procedure of interosseous dorsal antidromic artery flap, and could reduce the impact of frequency of anatomic variations and vascular complications.

Objective To establish a method for the determination of Paracetamol in Zihua Ganmao Capsule. Method Samples were separated on a Kromasil-C18 column with the mobile phase of methanol- water (35∶65), and the detection wavelength was set at 249 nm. Result The calibration curves were linear in the range of 0.165～0.99 ?g (r=0.999 9) for Paracetamol. The average recovery was 98.41% and RSD= 0.64% (n =6). Conclusion The method is accurate and repeatable, and can be used for the quality control of Zihua Ganmao Capsule.

Aim To study the effect of rhynchophylline on NMDA receptor 2B subunit(NR2B) mRNA expression in the nucleus accumbens(NAc) and amygdala(Amy) of amphetamine-induced condictioned place preference(CPP) rats.Method 56 male SPF grade Sprague-Dawley rats were randomly divided into control group,amphetamine-induced model group,model group with ketamine,model group with low,middle,high dose of rhynchophylline,and control group with rhynchophylline(n=8 for each group) The time of rats' staying in white compartment was measured in the 5th day,then NR2B mRNA expression in NAc and Amy of each rat was detected with in situ hybridization technology.Results The rats exhibited CPP after treated with 2 mg?kg-1?d-1 amphetamine for 4 d.Ketamine and rhynchophylline eliminated CPP effect,and the effect of rhychophylline showed a dose-dependence.In amphetamine-induced model group,NR2B mRNA expression in NAc and AMY of rats was significantly increased.Ketamine,middle and high dose of rhynchophylline blocked the increase of NR2B mRNA expression induced by amphetamine.Rhynchophylline had no effect on the basal level of RN2B mRNA expression in normal rats.Conclusion Inhibition of NR2B mRNA expression in NAc and AMY may contribute to the antagonistic effect of rhychophylline on amphetamine-induced CPP.

Aim To investigate the antagonistic action o f total saponins of panaxnotoginseng(PNS) on cardiac hypertrophy and its nervous mechanism.Methods (1)cardiac hypertrophy of rats due to pressure overload was induced by constricting of abdominal aorta. There were five groups in the experiments. The rats in Group A(control group)were sham operated . Group B was aorta-constricted group.The rats in Group C,D,E were given ip PNS 50,100,150 mg?kg?d -1 for three weeks respectively. Three weeks later, We measured the heart-weight(HW),left ventricular weight(LVW), the ratio of HW/BW,LVW/BW (LVI) and the cardiomyocyte diameters(MD) after dyeing by HE color.(2)The effects of PNS on the fast excitatory postsynaptic potential(f-EPSP),membrane depolarization induced by application of acetylcholine (ACh),membrane potential and membrane resistance of the isolated Stellate ganglion(SG)of the rats were investigated by means of intracellular recording techniques. Results (1)HW/BW, LVI and MD of Group E were significantly lower than that of Group B(P0.05).(2)At the concentration of 0.10 ～0.16 g?L -1, PNS reversibly depressed the amplitude of f-EPSP, but the ACh depolarization,membrane potential and membrane resistance were not significantly altered by PNS. Conclusion PNS can prevent cardiac hypertrophy due to pressure overload in rats and this action may underline its inhibitory action on presyn aptic effect of regulating calcium influx.

Objective To understand the implementation status and effect of prevention and control of malaria in Libo Coun?ty so as to provide the evidence for improving the malaria elimination working. Methods The data about malaria from the county CDC and county hospital were collected and 16 villages from 8 townships were randomly sampled and 10 villagers of each village were investigated. Other information about the prevention and control of malaria was also investigated. Results The inci?dence of malaria was decreasing annually from 5.75 per 10 000 in 2008 to zero in 2012. The malaria monitoring could be well conducted in the county and township levels. The infection source could be controlled in time. The utilization rate of anti?mosqui?to facilities in the residents was 93.25%and the awareness rate of knowledge about malaria prevention and control was 40.13%. Conclusions The implementation and effect of prevention and control of malaria are satisfactory in Libo County but the medi?um control is limited and the active protection consciousness of the residents is not strong. Therefore the task of malaria elimina?tion is still very arduous.

Gastroparesis is a clinical syndrome characterized by delayed gastric emptying of meal in the absence of mechanical obstruction of gastric outlet. In this article,the pathogenesis,etiology,epidemiology,clinical features, diagnosis and treatment of gastroparesis were reviewed.

The article introduces the network resource in urology surgery both at home and abroad and analyses the existing problems in carrying out online teaching uronology and puts forward some countermeasures.

Dead-in-bed syndrome (DBS) is a rare but devastating sudden death condition that mainly affects young patients with type 1 diabetes.Its mechanism is quite complicated and may involve multiple factors.Acute onset of recurrent and severe nocturnal hypoglycemia,abnormal cardiac repolarization,genetic factors,and obstructive sleep apnea may all contribute to ventricular arrhythmias and even sudden death.The future research plans include elucidating and understanding the underlying mechanisms and triggers for DBS,developing a risk stratification system,using real-time continuous glucose monitoring system to detect severe nocturnal hypoglycemia,and initiating appropriate therapeutic interventions to uhimately prevent the tragedy.