Objective To reveal the research focus of schistosomiasis in China through analyzing highly cited schistosomiasis related papers in journals from 2005 to 2012,so as to provide the information for formulating the plan of selecting topics of the edi-tors. Methods The published articles related to schistosomiasis in CNKI from 2005 to 2012 were collected,the citation frequen-cy of the papers was calculated according to formula,and the highly cited papers were selected according to the citation frequency. Then the information of highly cited papers including the published time,type,author and author affiliation,citation frequency, journal,grant and research item,research status and development tendency were analyzed. Results A total of 3 639 published papers related to schistosomiasis were searched and 343 papers(the citation frequency≥8)were selected as highly cited papers, and Chinese Journal of Schistosomiasis Control shared 45.19%of them. Among these papers,original articles,reviews and special reports occupied 44.90%,12.54%and 6.12%,respectively. The first authors of these papers were mainly from centers for disease control and prevention(CDCs)(17.33%),research institutions(22.67%)and colleges/universities(36.00%). Totally 50.44%of the articles received financial supports from research funds/foundations,and most of them were national or provincial funds. The research hotspots focused on epidemiology and prevention,epidemic situation analysis,vaccine,immunologic diagnosis,pharma-ceutical research,immunity and infection. Conclusions The highly cited papers related to schistosomiasis are mainly from CDCs,institutions and colleges/universities. The research hotspots mainly focus on epidemiology and prevention,vaccine,immu-nology and pharmaceutical study. The related journals can use this information to chose topic and solicit contributions to improve their influence.

Objective To evaluate the efficacy of telbivudine in HBeAg-positive chronic hepatitis B (CHB) patients by comparing the efficacy of initial telbivudine therapy in treatment-naive patients with sequential telbivudine therapy in patients with poor response to adefovir.Methods A total of 90 HBeAg-positive CHB patients were assigned to receive sequential telbivudine therapy following poor response to adefovir dipivoxil (n=45),or initial telbivudine therapy in antiviral treatment-naive patients (n=45).All patients were treated with telbivudine 600 mg daily for 104 weeks.The efficacy was evaluated in terms of liver function tests,serum HBV markers,HBV DNA and antiviral drug resistance.Results Telbivudine showed good overall efficacy after treatment for 104 weeks in terms of alanine aminotransferase normalization rate (91.1％),HBV DNA negative conversion rate (80.0％),HBeAg loss rate (57.8％),and HBeAg/HBeAb seroconversion rate (30.0％).The HBV DNA negative conversion rate in initial treatment group was significantly higher than that in sequential treatment group (P＜0.05).However,among the patients with early response,the efficacy did not show significant difference between groups (P＞0.05).The patients with early response showed significantly better efficacy than those without early response,in terms of higher HBV DNA negative conversion rate,higher HBeAg loss rate and HBeAg/ HBeAb seroconversion rate (P＜0.000 1 or P＜0.05),but lower virological breakthrough rate (P＜0.05).Conclusions Telbivudine has shown reliable efficacy in CHB patients.Initial telbivudine therapy is better than sequential therapy in CHB patients with poor response to adefovir.However,for patients with early response to telbivudine,no statistical difference is found between initial and sequential therapy in long-term treatment efficacy (104 weeks).The patients receiving sequential telbivudine therapy should be monitored closely for early antiviral response to optimize treatment.

Objective To investigate the indications to use a temporary pacemaker for bifascicular block in perioperative period.Methods The treatment for 40 patients with heart bifascicular block and a special case were retrospectively studied.All cases,according to their disease history,clinical symptoms,physical ability and atrio-ventricular conduction ability,were divided into two groups:P group(prophylactic insertion of a temporary pacemaker) and N group(without inserting a pacemaker).During operation,we observed whether atrio-ventricular block occurs,the performance of pacemaker and atropine's therapeutic efficacy to bradycardia.Results Anesthesia was postponed in one case because of acute left bundle branch block(LBBB) and the patient died with severe myocardium damage soon after failed resuscitation.All other cases went through anesthesia and operation smoothly.Bradycardia and hypotension could be relieved somewhat by medication in operation,and no complete atrio-ventricular block developed.Conclusion There is no need inserting a temporary pacemaker for chronic bifascicular block if asymptomatic and no atrio-ventricular block.Transesophageal atrial pacing is an easy way to evaluate in quantification atrio-ventricular conduction ability.

Objective To analyze the highly cited malaria papers published in Chinese journals from 2006 to 2013,so as to provide the evidence for formulating the plan of selecting topics to the journal editors. Methods The published articles related to malaria included in CNKI and Wanfang medical network from 2006 to 2013 were collected,and the highly cited papers were selected according to the citation frequency calculated by Price’s formula. Then the characteristics of the highly cited papers were analyzed. Result From 2006 to 2013,a total of 1 976 published papers related to malaria were searched in Chinese jour?nals and 98 papers of them were selected as highly cited papers. In the highly cited papers,18 papers were published in China Tropical Medicine,and 16 and 15 papers were published in Chinese Journal of Parasitology and Parasitic Diseases and Chi?nese Journal of Schistosomiasis Control,respectively;and original articles accounted for 42.86%;the first authors of these pa?pers were from 44 institutions,and 40.91%of them were from centers for disease control and prevention(CDCs);a percentage of 22.45%of the highly cited papers received fund programs,and most of them were national or provincial funds. The research hotspots were focused on the epidemiology and control,and epidemic situation of malaria. Conclusion The highly cited papers related to malaria are mainly from CDCs and research institutions,and the related journals could use this information to chose topics and solicit contributions to improve their influence.

Objective To reduce birthrate of severe thalassemia children of this area and improve population diathesis.Methods The red blood cell indices analysis was carried out on all of the samples of 2 218 couples.GapPCR and RDB method were used for α-thalassemia genotyping and β-thalassemia genotyping.Results 277 cases of thalassemia (12.49％) were identified among the total cases.220 cases were with α-thalassemia(9.92％),which including 198 cases of--SEA/αα,11 cases of-α37/,7 cases of-α4.2/αα,57 cases were with β-thalassemia(2.57％),the types of mutation were CD41/42 (-TTCT),IVS2nt-654 (C→T),CD17 (A→T),-28 (A→G),TATAbox29 (A→G),CD71/72(+ A).42 carrier couples were detected for thalassemia and the fetuses were subjected prenatal diagnosis:3cases of Bart's edema,7 cases of β-thalassemia homozygote.Conclusions Neonates with major thalassemia can be clarified and even avoided by screening the incidence and types of genicmutations.Thus setting up the system of prenatal screening-prenatal diagnosis-selective abortion is effective to avoid the birth of neonates.And it is vital to improve the quality of human being.

Nonaqueous capillary electrophoresis is used for the determination of the contents of diosgenin and ruscogenin in Radix Ophiopogonis. The operating buffer was composed of 20 mmol x L(-1) Na2B4O7-HCl (pH 7.61) in 70% methanol. The applied voltage was 25 kV and detection potential was at +0.70 V. With these conditions, the components were successfully separated. The content of diosgenin in Radix Ophiopogonis was 0.018 mg x g(-1) and ruscogenin was 0.008 mg x g(-1). The average recoveries of diosgenin and ruscogenin were 102% and 99.2%, respectively. A new method of the quality control of diosgenin and ruscogenin in Radix Ophiopogonis is provided.

Objective To analyze the predictive value of platelet related indicators for patent ductus arteriosus (PDA) in extremely low birth weight infants (ELBW). Methods The data of 79 ELBW infants born from June 2013 to June 2016 were retrospective analyzed. There were 48 cases without PDA (nPDA group) and 31 cases with PDA (PDA group). Among 31 cases with PDA, there were 17 cases of non-haemodynamically significant PDA (nhsPDA group) and 14 cases of haemodynamically significant PDA (hsPDA group). The clinical feature and platelet related indicators among nPDA group, PDA group, nhsPDA group and hsPDA group were compared. Multivariate logistic regression was used to analyze the effects of various factors on the occurrence of PDA. ROC curve analysis was performed to evaluate the early predictive value of platelet related indicators for PDA. Results Compared with the nPDA group, the PDA group had a smaller gestational age, a higher proportion of male infants, and a smaller platelet distribution width (PDW), and there were statistically significant differences in all of those (P all<0.05). Multivariate logistic regression analysis indicated that the risk of PDA was increased as the PDW was decreased (OR=1.26, 95%CI: 1.05~1.52). The ROC curve analysis showed that the best diagnostic value of PDW was 13.4 GSD, and the sensitivity of early prediction of PDA was about 67.74%, and the specificity was 68.75%. Compared with nhsPDA group, hsPDA group had a smaller gestation age, lower cesarean section rate, and there were statistically significant differences (P all<0.05). There was no significant difference in platelet related indicators between hsPDA group and nhsPDA group (P>0.05). Conclusion PDW has certain early predictive value for PDA in ELBW. ELBW infants with PDW<13.4 GSD need to be watched closely for the occurrence of PDA.

Objective To investigate the diagnosis value of soluble B7-H4(sB7-H4) in rheumatoid arthritis (RA) .Methods By using the ELISA sandwich method ,the serum levels of soluble B7-H4 ,anti-cyclic citrullinated peptide(anti-CCP) antibody and glu-cose 6-phosphate isomerase(GPI) were detected in 67 cases of RA ,72 cases of osteoarthritis(OA) and 75 cases of healthy people . The immunoturbidimetric method was adopted to determine the serum rheumatoid factor (RF) .Then the diagnostic value of sB7-H4 in RA was compared and analyzed .Results The serum level of soluble B7-H4 in the RA group was significantly higher than that in the control group and the OA group with statistically significant difference between them (P<0 .01) .The sensitivity of sB7-H4 in diagnosing RA was higher than that of RF ,anti-CCP antibody and GPI ,but the specificity was lower than that of anti-CCP antibody and GPI .There was better correlation between sB7-H4 with RF and anti-CCP antibody .The combination detecting of sB7-H4 ,RF , anti-CCP antibody and GPI could increase the diagnostic rate of RA .Conclusion B7-H4 has better clinical application prospects in diagnosing RA .

Cell Differentiation ; drug effects ; Dimethyl Sulfoxide ; pharmacology ; HL-60 Cells ; Humans ; Proteomics

Objective To investigate the usage of antibacterial drug in the department of operation in our hospital for improving the management and proper clinical utilization.Methods 1366 operational cases from our hospital were randomly collected from June 2004 to June 2005 and a comprehensive analysis of the usage of antibacterial drugs was conducted.Results All of the cases were given antibacterial drugs.The rate of preventive usage was 89.0%,among which those given in the period in 1h before operating was only 30.6%,and the other's was given after operating.The therapy time of antibacterial drugs were ranged from 1 to 63 days in different case and the preventive usage were 1 to 16 days.The therapy time of surpass 3 days was 94.7% and surpass 7 days was 53.5% in preventive usage.Prescriptions of more than one kind drug was widespread very much and 2～3 kinds were 87.5%.The wide-table,new-style and high-efficiency antibacterial drugs were the most frequently used in prescription.Conclusions The use of the antibacterial drugs in operational case in our hospital has some problem,especially in scope,opportunity moment,therapy time and union of usage.The management and education about rational usage of antibacterial drugs must be strengthened in order to improve the level of rationality in the clinical application.