Objective To explore the clinical effect and influence of hemocoagulase on bleeding control and coagulation function in neu -rosurgery .Methods A total of 300 patients with surgical treatment in our hospital from June 2013 to June 2014 were randomly divided into control group (n=150) and observation group (n=150).The patients in control group were treated with physiological saline 10 mL topical spraying,while the patients in observation group were treated with physiological saline 10 mL plus hemocoagulase 4U topical spraying.The clinical effect and coagulation index were compared between the two groups .Results The bleeding volume of the observation group was sig-nificantly lower than that of the control group (P<0.01).No significant difference was found in the coagulation index of the two groups (P>0.05).Conclusion Hemocoagulase treatment during the operation can reduce the volume of bleeding and transfusion of blood ,and do not affect the coagulation function in the patients .

0.05) except BSR ( P

Objective To probe the effect of perioperative therapeutic regime on breast reconstruction after surgery in breast cancer patients.Methods We retrospectively reviewed the clinical data of 145 consecutive breast cancer patients with 162 reconstructions.Results 127 of 145 patients got an excellent or good appearance (87.6％),and 42 cases had complications occurring in 162 operations (25.9％).After a median follow-up of 38.4 months,recurrences were found in 9 patients,3 cases died,and the disease free survival rate was 93.1％.Multivariate analysis showed that radiation therapy,without nipple-sparing and one-stage prosthesis implant were independent risk factors for negative postoperative aesthetic outcome;Delayed reconstruction and implant reconstruction were found to be protective factors for the postoperative complications.Conclusions Although the survival rate appears to be scarcely affected,different treatment modalities in reconstruction strategy bring different clinical results and outcomes.The perioperative decision-making of reconstruction strategy should be based on oncological safety,postoperative complications,aesthetic outcomes and subsequent therapies.

Humans ; Hyperamylasemia ; physiopathology ; Infant, Newborn ; Neonatology

Objective To investigate the clinic effect of trans-artery intrahepatic transplantation of porcine islet in type 1 diabetic patients. Methods Four patients with type 1 diabetes were perfused newborn porcine islets through the hepatic artery.Fasting blood glucose(FBS), postprandial blood glucose (PBS), HBA1,dose of insulin and function of liver and kidney were measured before and after the transplantation.Results The levels of FBS,PBS and HBA1 were 7 4～13 5mmol/L,7 8～18 6mmol/L and 7 8～12 0mmol/L respectively before the transplantation. During one and half years after transplantation, the levels of FBS,PBS and HBA1 were 3 3～7 1mmol/L,6 0～8 8mmol/L and 6 4～7 6mmol/L respectively with notably reduced dosage of insulin(32～58%).The function of liver and kidney of the patients remained normal.Conclusions Trans-artery intrahepatic transplantation of porcine islet is effective and safe for type 1diabetic treatment.

AIM: Based on the water-solubility of polyethylene glycol (PEG) with different relative molecular weight (RMW) and sodium alginate, we investigated the porous structure and property of calcium alginate scaffold with different RMW and dosages of PEG, which was used as porogenic agent. METHODS: The experiment was carried out in the Key Laboratory of Biomedical Engineering and Biomaterials, Jinan University from March 2006 to September 2007.①PEG at different RMWs (Mw=2 000, 4 000, 6 000, 8 000, 10 000, 20 000, 35 000) and different dosages (mass fraction=0, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06) were added into sodium alginate solution at 0.02 mass fraction. Then alginate was crosslinked, solidified and molded into indiscerptible film and graininess through Ca2+. PEG was dissolved by water. Therefore, a mass of porous structures could be formed in alginate.②Water content and swelling ratio of alginate was tested. Scanning electron microscope was used to observe the porous configuration. RESULTS: ①Porous alginate scaffold could be obtained through PEG and it exhibited good intensity and toughness, with water content reaching 92%. The scaffold could be formed into film and graininess.②Scanning electron microscope results revealed that the distribution of porous structure was uniformed. The aperture was 43.75 ?m-2.8 mm, and could be controlled by PEG's RMW and dosage. Inflated and uniform aperture structure was harvested when RMW of PEG was 4 000 and 6 000. CONCLUSION: Porous alginate structure can be obtained through regulating PEG's RMW and dosage. High water content and porosity of alginate scaffold material can be used in cell culture of tissue engineering and used as controlled release matrix of bio-active component.

Diamicron group. Conclusion Modified Taohe Chengqi Decoction and its diferent extractions maybe effective on raising or stabilizing the activity of Na+-K+-ATPase and Ca2+-ATPase in cadiocyte of diabetic rats, so it could prevent myocardium hypertrophy and patho-change by heart function failure in variably extent. Ethyl-acetate group and n-butyl alcohol group showed rather good effect among these extractions.

The human ?3-adrenoceptor plays an important role in the regulation of energy mobilization and utilization. A missense mutation in codon 64 of the gene for the ?3-adrenoceptor with a replacement of tryptophan to arginine (Trp64Arg) was found. This polymorphism influences receptor function when expressed in artificial cell lines or in isolated human fat cells. It is associated with an earlier onset of type 2 diabetes mellitus, features of insulin resistance and tendency to gain weight. Recent evidences suggest that Trp64Arg has additive and interactive effects with a number of other candidate gene variants. The functional significance of human ?3-adrenoceptor polymorphism and its association with metabolic diseases are reviewed in this paper.

All of the thrombolytic agents currently approved for use in humans are plasminogen activators, the application of which is limited by bleeding complications at vascular injury sites and plasminogen content in the thrombus. Plasmin is rapidly neutral-ized in the circulation by α2-antiplasmin and tolerated without bleeding. With the application of catheter-based delivery, the unique bio-chemical properties of plasmin make it a safe and effective direct fibrinolytics. Plasmin derivatives, including miniplasmin,Δ-plasmin and microplsmin, display more thrombolysis efficacy and better hemostatic safety in preclinical study and clinical trials. This review sum-marizes the current information on plasmin and its derivatives, including the advances on biochemical properties, preclinical and clinical trials.