Rhinitis,sinusitis,nasal polyps and bronchial asthma are common respiratory diseases,and they have some similarities in etiology,pathogenesis,histopathology and pathophysiology.Many patients suffer from these diseases in succession,or at the same time.Many domestic and international clinical and fundamental studies have aroused widespread concern about the relevance of these diseases,with the correlations between nasal polyps-sinusitis and asthma that attribute to much attention.However,the relevance of extent on the study of these diseases,as well as diagnosis,treatment,and concept is not very clear yet.The main target of this article is to discuss the above issues and remind our clinicians to pay more attention to understand the comprehensive relationships of rhinitis,sinusitis,nasal polyps and bronchial asthma,and provide references for the strategy of rational and effective treatment options.

Objective To study the technology of multi-parameter monitoring system based on ZigBee wireless network.Methods A asynchronous serial interface of IC CC2430 was used to receive the data of multi-parameter patient monitor,and then the ZigBee channels of IC CC2430 was sent to transmit the data to the central monitoring control system.Results The data of eight multi-parameter patient monitors was successfully transmitted to the central control system,and was not interfered by wireless transmission circuits.Conclusion ZigBee wireless network technology can be successfully applied to a wireless network of multi-parameter patient monitor control system.

Objective: To investigate the effects of gene silencing and overexpression of RACK 1 (receptor for activated C kinase 1) on the proliferation of large-cell lung cancer H460 cells and lung adenocarcinoma A549 cells, and to explore the possible mechanism. Methods: The RACK1 siRNA (small interfering RNA) targeting RACK 1 gene and recombinant vector pCMV-sport6-RACK1 were transfected into both of H460 cells and A549 cells, respectively. MTT method and colony formation assay were used to detect the effect of RACK 1 gene expression on the proliferation of lung cancer cells. Flow cytometry was used to examine the change of cell cycle. The association and interaction of RACK 1 gene expression with the proliferation of lung cancer cells were analyzed by yeast two-hybrid system, co-immunoprecipitation, laser scanning confocal microscopy and co-immunoprecipitation of phosphoproteins. Results: The expression levels of RACK1 protein in the H460 cells and A549 cells were both decreased after transfection with RACK1 siRNA, and the abilities of proliferation and colony-formation were also weakened. The proportion of lung cancer cells arrested at phase S was significantly declined (P <0.01). Meanwhile, the expression level of RACK1 protein was increased after transfection with pCMV-sport6-RACK1, and the abilities of proliferation and colony-formation of lung cancer cells were both strengthened with a prolonged doubling time. The proportion of lung cancer cells arrested at phase S was significantly increased (P <0.01). The results of yeast two-hybrid system and co-immunoprecipitation revealed that RACK1 could directly interact with MCM7 (minichromosome maintenance protein 7). The phosphorylation of MCM7 protein was strengthened through binding to RACK1 which translocated into the cell nucleus. Conclusion: RACK1 promotes the proliferation of lung cancer cells through activating the phosphorylation of MCM7 binding to RACK1. Copyright© 2012 by TUMOR.

OBJECTIVE: A im To investigate the role of qercetin(Que) -induced autophagy in the death of hepatocellular carcinoma cell line SMMC-7721 cells. METHODS: After treatment with different concentration of Que, the growth inhibition of the SMMC-7721 cells were assessed by MTT colorimetric assay. The fluorescent staining was applied to identify the autophagy after Que treatment. Lactate dehydrogenase (LDH) leakage and Western blot analysis were used to study the autophagic mechanisms involved in death of SMMC-7721 cells. RESULTS: The proliferation of SMMC-7721 cells were significantly inhibited in a dose and time-dependent manner after Que treatment. Autophagy was also induced in MCF-7 cells as detected by MDC staining and Fluorescent staining in the early phase. The autophagy specific inhibitor 3-MA or chloroquine potentiated Que's cytotoxicitiy in SMMC-7721 cells when administered 1h before Que; The expression of cathepsin B increased after Que treatment. CONCLUSION: Que can significantly inhibit the growth of the SMMC-7721 cells by inducing the autophagy, which is a protection mechanism that can reduce the cytotoxicity induced by Que in SMMC-7721 cells. The activation of Cathepsin B is considerable in autophagy process.

Objective To investigate the clinical effects of retroperitoneal laparoscopic surgery in the management of diseases of adrenal glands, kidneys and ureters. Methods By using a closed puncture into retroperitoneal space to establish a surgical access ,87 patients with diseases of adrenal glands, renal cysts, ureteral calculi and ureteral deformities were operated on by means of retroperitoneal laparoscopic surgery. Results The surgery was successfully accomplished in 83 patients with an excellent therapeutic effect, whereas a conversion to open surgery was required in 4 patients. There were no blood transfusions or serious complications in the study. Conclusions Retroperitoneal laparoscopic operations of adrenalectomy, cyst unroofing, ureterolithotomy, ureterolysis and ureteroplasty has the advantages of minimal invasion, less blood loss and fewer complications, being an effective and practical procedure .

Objective To discuss the risk factors for occurrence of acute gout in the patient with hyperuricement so that the countermeasure can be made.Methods The data of routine health examination was collected and questionnaire was made in 1847 hyperuricemic patients.The single factor analysis and the Logistic regression analysis were used to screen the risk factors for acute gout such as age,sex,course of diseases,FBS,lipid,uric acid,and complicationas.Results Sex,BMI,positive family history and complication with hypertension and hyperlipidemiaand have the siginificantly influence on the attack of acute gout in hyperuricemic patients.Conclusion The nurse should know very well the risk factors for acute gout and its induction factors,strengthen patient's health education and make different measures for different factors.

Objective:To use the perineum four-dimensional pelvic ultrasound stereo imaging for detecting injury situation of pelvic diaphragm hiatus and anal levator ani muscle. Understand abnormal classification of pelvic floor muscle. investigate the application of four-dimensional pelvic ultrasound stereo imaging in pelvic floor dysfunction (PFD) for primipara in order to provide more accurate data for clinical practice.Methods: 270 primiparas with pelvic floor dysfunction were divided into the vaginal delivery group (135 cases) and selective cesarean group (135 cases) as random number table; pelvic floor muscle strength, longitudinal diameter, transverse diameter, area of pelvic diaphragm hiatus and thickness of the anus levator were detected by the perineum four-dimensional pelvic ultrasound stereo imaging, respectively, and then to compare differences between the two groups. Results: In 270 cases, 162 cases (60%) were abnormalities in pelvic floor muscle strength, and 189 cases (70%) were abnormalities in vaginal pressure; the results of pelvic floor muscles fatigue grade situation revealed: 127 cases were abnormal muscle fiber strength ofⅠtype (47%), 30 patients were abnormal muscle fiber strength ofⅡ type (11%). There were no significant differences between the two groups in the abnormal rate of pelvic floor muscle strength, vaginal pressure and muscle fiber strength. Longitudinal diameter, transverse diameter and area of pelvic diaphragm hiatus in the vaginal delivery group were higher than that in the selective cesarean group, while thickness of anal levator were thinner than that in the selective cesarean group with significant differences (t=3.730,t=3.467, t=3.826,t=1.809;P<0.05).Conclusions: The perineum four-dimensional ultrasound diagnosis can intuitively and clearly show morphological characteristics of female pelvic diaphragm hiatus, therefore, it can quickly finish diagnosis, and provide the basis for clinical diagnosis and treatment evaluation of postpartum PFD.

Objective To observe the effect of mirror therapy and sensory reeducation on sensory function recovery after thumb recon-struction with second toe transplantation. Methods Forty patients after thumb reconstruction with second toe transplantation from January, 2011 to January, 2014 were divided into control group (n=20) and training group (n=20). Both groups accepted routine nursing and rehabili-tation, while the training group accepted mirror therapy and sensory reeducation training in addition. They were followed up for six to eigh-teen months after operation (average 9.3 months) with the two-point discrimination (2-PD) of transplanted toe pulp. Results 2-PD was (7.15±1.15) mm in the training group, very shorter than (10.23±2.23) mm of the control group (t=5.4898, P<0.001). Conclusion The mirror therapy combined with sensory reeducation training can improve the sensory function of the finger pulp that transplanted from the second toe.

Radiotherapy (RT) is the primary treatment for nasopharyngeal carcinoma (NPC). In order to achieve tumor control, nearby normal brain tissues, especially the temporal lobes, often receive unnecessary radiation. Patients with NPC treated by RT may occur cognitive impairments, which significantly compromises the quality of life. The symptoms of cognitive dysfunction induced by RT in NPC patients can exhibit at the early or later stage after RT, which may be related to temporal lobe injury or brain damage induced by radiation. However, the relationship between the cognitive declines induced by RT and the radiation dose or volume of the temporal lobe (or the dosimetry to treat NPC) has not yet been definitively established, and few reports have focused on cognitive impairment following intensity modulated RT. Therefore, this paper will review the progress in radiation-induced cognitive decline of NPC patients in order to provide the basis for preventing side effects of RT and improving the quality of life after RT.