BACKGROUND: Increasing degrees of medullary hyperemia induced by ischemia reperfusion injury were associated with renal dysfunction. A possible mechanism may be that ischemia causes alterations in the structure and function of vascular membranes which leads to an aggregation of red blood cells in the medullary vessel. It has been shown that heparin prevents postischemic endothelial cell dysfunction. Aim of this study was to evaluate heparin effects on renal hyperemia induced by ischemia reperfusion injury. METHOD: In this study, fifteen rabbits were randomized to either heparin treatment group(500 IU/kg IV bolus 10 minutes before renal artery occlusion, n=8) or control group(n=7). One side kidney underwent 60 minutes ischemia only by clamping renal pedicle and after that kidney tissue sample was obtained for histologic evaluation. The other side of kidney were permitted 60 minutes ischemia following 60 minutes reperfusion and after that kidney tissue sample was obtained for histologic evaluation. RESULTS: There was significant difference in the degree of congestion(2.6+/-0.2 vs 1.1+/-0.3, P<0.05) between outer medulla of control and heparin treatment group. CONCLUSION: Heparin significantly attenuated outer medullary congestion induced ischemic injury.
Constriction ; Endothelial Cells ; Erythrocytes ; Estrogens, Conjugated (USP) ; Heparin* ; Hyperemia ; Ischemia* ; Kidney ; Membranes ; Rabbits ; Renal Artery ; Reperfusion Injury ; Reperfusion*

This study examined 60 children with ADHD through K-ABC, ADDES-HV, ADD-H comprehensive teacher's rating scale (ACTeRS), CAP, SNAP, Academic Performance rating scale, K-PIC, CPT and WCST in order to estimate the effects of methylphenidate, and find out various factors which can influence on treatment responses and predictive factors of treatment. Boys showed better improvement in impulsiveness of behavior symptoms than girls, but girls showed better improvement in trials to completed the first category in WCST. In the aspect of age, the lower graders (age 7-9) showed improvement in sequential processing ability of intelligence but the higher graders (age 10-12) showed deterioration. In the aspect of sub-categories of ADHD, children with mixed type who had been worse in attention deficit and behavior symptoms in pretreatment state, showed significantly higher improvement than children with predominantly inattentive type in every scopes of behavior symptom check list. And in clusters of K-PIC, after treatments the group those who had have more anxiety or depression showed better improvement in omission error in CPT. In the aspect of intelligence level, children with average intelligence showed better improvement in impulsiveness and impulse control than the rests. In the aspect of executive functions, the less omission errors in CPT one made, the better improvement in attention and academic ability he showed. And the less perseverative response in WCST one had, the better improvement in hyperactivity he showed however his simultaneous processing abilities were deteriorated. With these results, this study confirms that methylphenidate is effective in behavior symptoms, academic performance, intelligence and various performance abilities of children with ADHD including attention and response control. In the cases of the children with severe ADHD symptoms, especially behavioral disinhibition and the children with mixed type of ADHD, it is considered useful to predict the results of treatments.
Anxiety ; Attention Deficit Disorder with Hyperactivity* ; Child* ; Depression ; Executive Function ; Female ; Humans ; Intelligence ; Methylphenidate

Sclerosing mesenteritis is a rare benign disease originated from the mesenteries. It can be related to autoimmune disease, vasculitis, ischemia, infection, trauma and operation, but most of cases are idiopathic. The overall prognosis of sclerosing mesenteritis is usually good with benign, course. However, no consensus of treatment has yet been established. We report a case of spontaneous partial regression of sclerosing mesenteritis presented as a huge mass and diagnosed by finding of contrast enhanced abdominal computed tomography and percutaneous ultrasonography guided needle biopsy.
Biopsy, Fine-Needle ; Diagnosis, Differential ; Humans ; Male ; Mesentery ; Middle Aged ; Panniculitis, Peritoneal/*diagnosis/pathology/ultrasonography ; Remission, Spontaneous ; Tomography, X-Ray Computed

BACKGROUND: The classical treatment of the cervical cancer is surgery, radiotherapy, chemotherapy. Even though the improvement of treatment successful rate, conventional therapy has some limitations. Recent cutting edge of cancer therapy has been developed in gene level including understand the biological characteristics of the cancer cells, enhance the human immune response, suppress the cancer cell proliferation. Therefore, the gene therapy is proposed to new treatment strategy. PURPOSE: The transfection efficiency of cervical cancer cell lines and cervical cancer cell line xerografted nude mouse was investigated by transfection of liposome and infection of adenovirus mediated suppressor(p53) and reportor(LacZ) gene. METHOD: The cervical cancer cell lines was used in this study were CaSki, SiHa (HPV16 positive, wild type p53 gene), HeLa, HelaS3(HPV18 positive, wild type p53 gene) and C33A, HT3(HPV negative, mutant p53). Direct plasmide and AdCMVp53 gene transfection was performed by using liposome system (pRcCMVLacZ / lipofectin, FuGene 6, Ca-phosphate). LacZ gene was used as the reportor gene for the transfection efficiency evaluation. Expression of p53 in cell lines and tumor tissue was confirmed by western blot and immunohistochemical staining. Xenografted nude mouse of SiHa cell line was infected by AdCMVp53 and AdCMVLacZ. Transfection efficiency was observed by same as above. RESULTS: In cervical cancer cell lines, gene transfection using liposome system(pRcCMVLacZ/lipofectin, FuGene 6, Ca-phosphate)revealed different transfection efficiency, especially pRcCMVLacZ in Fugene 6 showed 18-40% of high transfection efficiency in 6 cervical cancer cell lines by X-gal staining and AdCMVp53 showed 95-98% of the high transfection efficiency in HeLa, C33A. AdCMVp53 was significantly expressed at 2-5days after injection xenografted nude mouse on the western blot and transfection efficiency was 19.79+/-5.36, 26.26+/-11.69, 14.77+/-3.98,15.99+/-6.43%(day1-5). AdCMVLacZ were found to immuno- histochemistry analysis, in vivo transfection efficiency was 61.26+/-4.66,59.63+/-9.12, 29.46+/-14.33, 31.73+/- 22.64%(day 1-5) atx200 and 88.65+/-8.65, 70.85+/-20.94, 40.75+/-25.44, 48.21+/-10.97% (day 1-5) atx400. CONCLUSION: As a results, adenovirus-mediated transfection efficiency was higher in vivo experiment compared to cell lines. These high efficiency of adenovirus-mediated suppressor gene(p53) could become a significant meaningful data gene therapy strategy both transgenic mice and cervical cancer cell lines.
Adenoviridae* ; Animals ; Blotting, Western ; Cell Line* ; Cell Proliferation ; Drug Therapy ; Genes, p53* ; Genetic Therapy ; Heterografts* ; Humans* ; Lac Operon ; Liposomes ; Mice ; Mice, Nude* ; Mice, Transgenic ; Plasmids ; Population Characteristics ; Radiotherapy ; Transfection* ; Uterine Cervical Neoplasms*