During the study of soil mycoflora in Jinju-shi in 1997, a dematiaceous hyphomycete, Gonytrichum macrocladum , was isolated using the soil dilute plating method. The isolate was recovered with very low frequencies and recorded for the first time in Korea. illustrated descriptions are presented for the isolate examined in the present study.
Korea* ; Mitosporic Fungi* ; Soil*

While studying Bombyx mori "Zipnuae" inoculated with an entomopathogenic fungus, Paecilomyces tenuipes, a mycoparasitic hymenoascomycete with a long neck was isolated from the silkworm rearing room. The fungus was identified as Syspastospora parasitica which has been recognized as a hyperparasite on various moniliaceous hyphomycetes. A descriptive illustration is presented in this study.
Ascomycota* ; Bombyx* ; Fungi* ; Mitosporic Fungi ; Neck ; Paecilomyces*

Adolescent ; Female ; Humans ; Hypersensitivity ; microbiology ; Mitosporic Fungi ; Sinusitis ; microbiology

OBJECTIVETo isolate and identify the high-yield poly-malic acid (PMLA) bacterial strains from the nature.

METHODSSamples were collected and cultured. The high-yield PMLA bacterial strains were screened through morphological observation, qualitative PMLA tests by HPLC and ITS sequence analysis on the isolated bacterial strains.

RESULTSA high-yield PMLA strain II 04 was isolated, the yield of PMLA of the strain reached to 26.23g/L in the rotary shaker at 25 degree for 7d. From morphological observation and ITS sequences analysis, the strain belonged to Aureobasidium pullulans, and named as Aureobasidium pullulans ZUCC-41.

CONCLUSIONA high-yield bacterial strain has been isolated from the nature and identified to be Aureobasidium pullulans.

Fermentation ; Malates ; metabolism ; Mitosporic Fungi ; isolation & purification ; metabolism ; Polymers ; metabolism

The Kingdom fungus has a unique structure and organization. Recent advances in electron microscopy and use of specific cytochemical technique enable the ultrastructures to be visualized. The hypha is a tube-like structure with a rigid wall, containing a moving slug of protoplasm. Hypha grows only at the tapered apical tip region, which is called extension zone. Extreme tip area has apical vesicle cluster which is responsible for tip growth. Unique fungal structure, Spitzenk rper, is thought to be a central region of the apical vesicle cluster. Most hyphal structures except the species belong to Zygomycetes have septa. But the septum is not completely blocked and it has different types of opening pores. The simple septal pores with Woronin bodies, which are found in Ascomycetes and Deuteromycetes, can be plugged in two different mechanisms. During normal differentiation the pores become occluded by a gradual deposition of plugged material. Loss of cytoplasm from damaged hyphae can be reduced and blocked by the rapid occlusion of septal pores by Woronin bodies or hexagonal crystal bodies. Septal sealing in Basidiomycetes which have dolipore septum is made by the rapid formation of electron-dense pore plugs. The shape of the fungal cell is the shape of fungal wall. Fungal walls appear to be composed of layers, which are thought to merge into one another to form one structure. The cytoskeleton consists of microtubules and microfilaments with motor proteins, and they seems to act together in the fungal cells.
Actin Cytoskeleton ; Ascomycota ; Basidiomycota ; Cytoplasm ; Cytoskeleton ; Fungal Structures ; Fungi ; Gastropoda ; Hyphae ; Microscopy, Electron ; Microtubules ; Mitosporic Fungi

Fungi constitute nonmotile eukaryotic organism that are devoid of chlorophyll and reproduce by spores. There are 100, 000 validly described species of fungi and over 275 species of those described fungi have been reported to cause disease in man and animals. Molds called filamentous fungi belong to the class Hyphomycetes. In the identification of Molds, the cultures have been purified and then observe their colony characteristics and microscopic morphology. Visual colony examination is important identifying marker concerning color, texture, diffusible pigments, exudates, macroscopic structures, growth rates and colony topography. In addition to the overall appearance of the colony, final identification should be made by microscopic examination. Microscopic features provide by the asexual/sexual spores, cells per spore, color of spores, spore formation and fragmentation of hyphae. Biochemical tests are not as important as markers for identifying filamentous fungi as they are for yeasts and dermatophytes.
Animals ; Arthrodermataceae ; Chlorophyll ; Exudates and Transudates ; Fungi* ; Hyphae ; Mitosporic Fungi ; Spores ; Yeasts

During the study of microbial structures in root-regions of tomato and red pepper from fields, various soil-inhabiting fungi were isolated with the dilution plate technique. Among them an ascomycete, Emericellopsis mirabilis and three hyphomycetes, Gliocladium solani, Humicola veronae and Verticillium chlamydosporium are presented for the first time in Korea along with Talaromyces trachyspermus, Chaetomium globosum and Doratomyces microsporus.
Ascomycota ; Capsicum ; Chaetomium ; Classification ; Fungi* ; Gliocladium ; Korea* ; Lycopersicon esculentum ; Mirabilis ; Mitosporic Fungi ; Talaromyces ; Verticillium

A severe leaf spot, turning to foliage blight, was observed on leaves of Maranta leuconeura growing in a garden in Brazil (state of Rio de Janeiro) in 2015. A dematiaceous hyphomycete bearing a morphology typical of a helminthosporoid fungi was regularly found in association with diseased tissues. The fungus was isolated and pathogenicity was demonstrated through the completion of Koch's postulates. A morphology and molecular analysis led to the conclusion that the fungus belonged to the genus Bipolaris, which is characterized by having fusiform conidia, externally thickened and truncate hila and a bipolar pattern of germination. Additionally, homology of internal transcribed spacer and GAPDH sequences with sequences of other Bipolaris species, confirmed its generic placement. A phylogenetic study also indicated clearly that the fungus on M. leuconeura is phylogenetically distinct from related species of this genus, leading to the proposal of the new species Bipolaris marantae.
Agriculture* ; Brazil* ; Classification ; Fungi ; Germination ; Marantaceae* ; Mitosporic Fungi ; Phylogeny ; Plants* ; Spores, Fungal ; Virulence

AIMTo investigate the effects of fungal elicitors on inophyllums production in suspension cultured cell of Calophyllum inophyllum Linn.

METHODSThe pathogen of leaf spot disease of C. inophyllum L. was isolated and prepared as fungal elicitor. The fungal elicitor was added to the medium with different concentrations and culture period. Their effects on biomass and inophyllums content of the suspension of cultured cells were studied.

RESULTSThe optimum effects of S-I fungal elicitor concentrations on inophyllums content was 60 mg GE x L(-1). Adding the fungi elicitor into the cell suspension culture system at stationary phase (being cultured for 18 days) resulted in a highest inophyllum content of 59.174 mg x L(-1) at the 3rd day with 27% higher than control. Fungal elicitor treatment promoted the inophyllums accumulation in medium.

CONCLUSIONAdding the Stagonospora curtisii (Berk.) Sacc. to the medium was effective approaches to enhance inophyllums yield in the suspension of C. inophyllum L culture cell.

Calophyllum ; cytology ; metabolism ; Cell Division ; Cells, Cultured ; Coumarins ; metabolism ; Culture Media ; Mitosporic Fungi ; physiology

Produced by and purified from Taxus brevifolia, Taxol (paclitaxel) has become a widely used cancer drug in clinic. Due to the rapid growing market, current industrial production of taxol by semi-synthesis that consumes large amount of Taxus trees cannot meet the requirement of the market. The discovery of taxol-producing fungus Taxomyces andeanae, an endophyte of T. brevifolia, by Stierle et al (1993), paves a new way to the production of the drug, i.e. employing large-scale fungal fermentation to make Taxol at lower cost and yet higher yield. This review discusses the present problems in taxol production in pharmaceutical industry, the finding and research progress on taxol-producing fungi, and the potential application of fungal fermentation to manufacture this important drug.
Antineoplastic Agents, Phytogenic ; biosynthesis ; Fermentation ; Mitosporic Fungi ; metabolism ; Paclitaxel ; biosynthesis ; Taxus ; microbiology ; Technology, Pharmaceutical ; methods