1.Research progress on the effect of mitochondrial network remodeling on macrophages.
Lianlian ZHU ; Xiangmin KONG ; Wei ZHU
Chinese Journal of Cellular and Molecular Immunology 2023;39(7):656-662
Remodeling of the mitochondrial network is an important process in the maintenance of cellular homeostasis and is closely related to mitochondrial function. Interactions between the biogenesis of new mitochondria and the clearance of damaged mitochondria (mitophagy) is an important manifestation of mitochondrial network remodeling. Mitochondrial fission and fusion act as a bridge between biogenesis and mitophagy. In recent years, the importance of these processes has been described in a variety of tissues and cell types and under a variety of conditions. For example, robust remodeling of the mitochondrial network has been reported during the polarization and effector function of macrophages. Previous studies have also revealed the important role of mitochondrial morphological structure and metabolic changes in regulating the function of macrophages. Therefore, the processes that regulate remodeling of the mitochondrial network also play a crucial role in the immune response of macrophages. In this paper, we focus on the molecular mechanisms of mitochondrial regeneration, fission, fusion, and mitophagy in the process of mitochondrial network remodeling, and integrate these mechanisms to investigate their biological roles in macrophage polarization, inflammasome activation, and efferocytosis.
Mitochondria
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Mitophagy
;
Homeostasis/physiology*
;
Phagocytosis
;
Macrophages/metabolism*
2.Progress in regulation of mitochondrial dynamics and mitochondrial autophagy.
Jing CHENG ; Lin WEI ; Miao LI
Acta Physiologica Sinica 2020;72(4):475-487
The abnormality of mitochondrial morphology and function is closely related to the pathogenesis of many diseases. Mitochondrial fusion-fission dynamics are critical to maintain normal morphology, distribution and quantity of mitochondria, and ensure the normal activity of cells. In addition, mitochondrial autophagy (mitophagy) plays an important role in maintaining mitochondrial quality by degrading aging or damaged mitochondria. Many previous studies showed that mitochondrial dynamics and mitophagy can regulate each other to sustain mitochondrial network homeostasis. Clarifying regulatory mechanisms of mitochondrial dynamics and mitophagy is of great significance for revealing the molecular mechanism of various diseases and for the development of new drugs targeting mitochondrial dynamics proteins or mitophagy regulatory proteins. This review focuses on the role of mitochondrial dynamics and mitophagy in mitochondrial quality control, regulatory mechanism, the interplay between those two processes, and their roles in human-related diseases.
Autophagy
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Humans
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Mitochondria
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Mitochondrial Dynamics
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Mitochondrial Proteins
;
Mitophagy
3.Taurine inhibits M2 polarization of macrophages by promoting mitophagy.
Chengying CHEN ; Chunhua LAN ; Jianglang YUAN ; Xingxing KONG ; Li LAN ; Xinhang WANG ; Shengboxiaoji CHANG ; Cailing LU ; Xiyi LI ; Shen TANG
Chinese Journal of Cellular and Molecular Immunology 2023;39(6):488-493
Objective To investigate the molecular mechanism of taurine regulating the polarization of M2 macrophages by mitophagy. Methods THP-1 cells were divided into four groups: M0 group (THP-1 cells were treated by 100 nmol/L phorbol myristate ester for 48 hours to polarize into M0), M2 group (THP-1 cells were induced to polarize into M2 macrophages by 20 ng/mL interferon-4 (IL-4) for 48 hours), M2 combined with taurine groups (added with 40 or 80 mmol/L taurine on the basis of M2 macrophages). The mRNA expression of mannose receptor C type 1(MRC-1), C-C motif chemokine ligand 22(CCL22) and dendritic cell-specific ICAM-3 grabbing non-integrin (CD209) in M2 macrophages were detected by quantitative real-time PCR. Mitochondrial and lysosome probes were used to detect the number of mitochondria and lysosomes by multifunction microplate reader and confocal laser scanning microscope. The level of mitochondrial membrane potential (MMP) was detected by JC-1 MMP assay kit. The expression of mitophagy-related proteins PTEN-induced putative kinase 1 (PINK1) and microtubule-associated protein 1 light chain 3 (LC3) were detected by Western blot analysis. Results Compared with M0 group, the expression of MRC-1, CCL22, CD209 and PINK1, the number of mitochondria and the level of MMP in M2 group were significantly increased, whereas the number of lysosomes and LC3II/LC3I ratio were decreased. Compared with M2 group, the expressions of MRC-1, CCL22 and CD209, the number of mitochondria and the level of MMP in M2 combined with taurine group dropped significantly while the number of lysosomes was found increased, and the protein expression of PINK1 and LC3II/LC3I ratio were also increased. Conclusions The polarization of M2 macrophages is regulated by taurine to prevent excessive polarization via reducing the level of MMP, improving the level of mitophagy, reducing the number of mitochondria, and inhibiting the mRNA expression of polarization markers in M2 macrophages.
Mitophagy
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Taurine
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Macrophages/metabolism*
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Protein Kinases/metabolism*
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RNA, Messenger
4.Critical hubs of renal ischemia-reperfusion injury: endoplasmic reticulum-mitochondria tethering complexes.
Huan-Huan ZHAO ; Qiu-Xia HAN ; Xiao-Nan DING ; Jing-Yao YAN ; Qi LI ; Dong ZHANG ; Han-Yu ZHU
Chinese Medical Journal 2020;133(21):2599-2609
Mitochondrial injury and endoplasmic reticulum (ER) stress are considered to be the key mechanisms of renal ischemia-reperfusion (I/R) injury. Mitochondria are membrane-bound organelles that form close physical contact with a specific domain of the ER, known as mitochondrial-associated membranes. The close physical contact between them is mainly restrained by ER-mitochondria tethering complexes, which can play an important role in mitochondrial damage, ER stress, lipid homeostasis, and cell death. Several ER-mitochondria tethering complex components are involved in the process of renal I/R injury. A better understanding of the physical and functional interaction between ER and mitochondria is helpful to further clarify the mechanism of renal I/R injury and provide potential therapeutic targets. In this review, we aim to describe the structure of the tethering complex and elucidate its pivotal role in renal I/R injury by summarizing its role in many important mechanisms, such as mitophagy, mitochondrial fission, mitochondrial fusion, apoptosis and necrosis, ER stress, mitochondrial substance transport, and lipid metabolism.
Endoplasmic Reticulum/metabolism*
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Endoplasmic Reticulum Stress
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Humans
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Mitochondria
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Mitochondrial Membranes/metabolism*
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Mitophagy
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Reperfusion Injury/metabolism*
5.Advances in the study of mitophagy-related receptor proteins.
Rang-Gui MA ; Zhi XIA ; Hua-Yu SHANG
Acta Physiologica Sinica 2021;73(6):1025-1034
Cells selectively scavenge redundant or damaged mitochondria by mitophagy, which is an important mechanism of mitochondrial quality control. Recent studies have shown that mitophagy is mainly regulated by autophagy-related genes (Atgs) in yeast cells, while mitochondrial membrane associated proteins such as PTEN-induced putative kinase 1 (PINK1), NIX/BNIP3L, BNIP3, FUN14 domain containing 1 (FUNDC1), FKBP8/FKBP38, Bcl-2-like protein 13 (Bcl2L13), nucleotide binding domain and leucine-rich-repeat-containing proteins X1 (NLRX1), prohibitin 2 (PHB2) and lipids such as cardiolipin (CL) are the key mitophagic receptors in mammalian cells, which can selectively recognize damaged mitochondria, recruit them into isolation membranes by binding to microtubule-associated protein 1 light chain 3 (LC3) or γ-aminobutyric acid receptor-associated protein (GABARAP), and then fuse with lysosomes to eliminate the trapped mitochondria. This article reviews recent research progress of mitophagy-related receptor proteins.
Animals
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Apoptosis Regulatory Proteins
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Autophagy
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Microtubule-Associated Proteins
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Mitochondria
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Mitochondrial Proteins/genetics*
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Mitophagy
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Prohibitins
6.Korean red ginseng decreases 1-methyl-4-phenylpyridinium-induced mitophagy in SH-SY5Y cells.
Hyongjun JEON ; Hee-Young KIM ; Chang-Hwan BAE ; Yukyung LEE ; Sungtae KOO ; Seungtae KIM
Journal of Integrative Medicine 2021;19(6):537-544
OBJECTIVE:
Mitophagy is known to contribute towards progression of Parkinson's disease. Korean red ginseng (KRG) is a widely used medicinal herb in East Asia, and recent studies have reported that KRG prevents 1-methyl-4-phenylpyridinium ion (MPP
METHODS:
SH-SY5Y cells were incubated with KRG for 24 h, and subsequently exposed to MPP
RESULTS:
MPP
CONCLUSION
KRG effectively prevents MPP
1-Methyl-4-phenylpyridinium/toxicity*
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Apoptosis
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Cell Line, Tumor
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Mitochondria
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Mitophagy
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Panax
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Reactive Oxygen Species
7.Effect on Danggui Shaoyao Powder on mitophagy in rat model of Alzheimer's disease based on PINK1-Parkin pathway.
Miao YANG ; Wen-Jing YU ; Chun-Xiang HE ; Yi-Jie JIN ; Ze LI ; Ping LI ; Si-Si DENG ; Ya-Qiao YI ; Shao-Wu CHENG ; Zhen-Yan SONG
China Journal of Chinese Materia Medica 2023;48(2):534-541
This study investigated the mechanism of Danggui Shaoyao Powder(DSP) against mitophagy in rat model of Alzheimer's disease(AD) induced by streptozotocin(STZ) based on PTEN induced putative kinase 1(PINK1)-Parkin signaling pathway. The AD rat model was established by injecting STZ into the lateral ventricle, and the rats were divided into normal group, model group, DSP low-dose group(12 g·kg~(-1)·d~(-1)), DSP medium-dose group(24 g·kg~(-1)·d~(-1)), and DSP high-dose group(36 g·kg~(-1)·d~(-1)). Morris water maze test was used to detect the learning and memory function of the rats, and transmission electron microscopy and immunofluorescence were employed to detect mitophagy. The protein expression levels of PINK1, Parkin, LC3BⅠ/LC3BⅡ, and p62 were assayed by Western blot. Compared with the normal group, the model group showed a significant decrease in the learning and memory function(P<0.01), reduced protein expression of PINK1 and Parkin(P<0.05), increased protein expression of LC3BⅠ/LC3BⅡ and p62(P<0.05), and decreased occurrence of mitophagy(P<0.01). Compared with the model group, the DSP medium-and high-dose groups notably improved the learning and memory ability of AD rats, which mainly manifested as shortened escape latency, leng-thened time in target quadrants and elevated number of crossing the platform(P<0.05 or P<0.01), remarkably activated mitophagy(P<0.05), up-regulated the protein expression of PINK1 and Parkin, and down-regulated the protein expression of LC3BⅠ/LC3BⅡ and p62(P<0.05 or P<0.01). These results demonstrated that DSP might promote mitophagy mediated by PINK1-Parkin pathway to remove damaged mitochondria and improve mitochondrial function, thereby exerting a neuroprotective effect.
Rats
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Animals
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Mitophagy
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Alzheimer Disease/genetics*
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Powders
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Protein Kinases/metabolism*
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Ubiquitin-Protein Ligases/metabolism*
8.Role of Mitophagy in Myocardial Ischemia/Reperfusion Injury and Chinese Medicine Treatment.
Jun-Yan XIA ; Cong CHEN ; Qian LIN ; Jie CUI ; Jie WAN ; Yan LI ; Dong LI
Chinese journal of integrative medicine 2023;29(1):81-88
Mitophagy is one of the important targets for the prevention and treatment of myocardial ischemia/reperfusion injury (MIRI). Moderate mitophagy can remove damaged mitochondria, inhibit excessive reactive oxygen species accumulation, and protect mitochondria from damage. However, excessive enhancement of mitophagy greatly reduces adenosine triphosphate production and energy supply for cell survival, and aggravates cell death. How dysfunctional mitochondria are selectively recognized and engulfed is related to the interaction of adaptors on the mitochondrial membrane, which mainly include phosphatase and tensin homolog deleted on chromosome ten (PTEN)-induced kinase 1/Parkin, hypoxia-inducible factor-1 α/Bcl-2 and adenovirus e1b19k Da interacting protein 3, FUN-14 domain containing protein 1 receptor-mediated mitophagy pathway and so on. In this review, the authors briefly summarize the main pathways currently studied on mitophagy and the relationship between mitophagy and MIRI, and incorporate and analyze research data on prevention and treatment of MIRI with Chinese medicine, thereby provide relevant theoretical basis and treatment ideas for clinical prevention of MIRI.
Humans
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Mitochondria/metabolism*
;
Mitophagy/genetics*
;
Myocardial Reperfusion Injury
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Protein Kinases/metabolism*
9.Effect of mitophagy related genes on the antioxidant properties of Saccharomyces cerevisiae.
Wanqi CHENG ; Qianyao HOU ; Chunfeng LIU ; Chengtuo NIU ; Feiyun ZHENG ; Qi LI ; Jinjing WANG
Chinese Journal of Biotechnology 2023;39(8):3464-3480
Mitophagy is a process whereby cells selectively remove mitochondria through the mechanism of autophagy, which plays an important role in maintaining cellular homeostasis. In order to explore the effect of mitophagy genes on the antioxidant activities of Saccharomyces cerevisiae, mutants with deletion or overexpression of mitophagy genes ATG8, ATG11 and ATG32 were constructed respectively. The results indicated that overexpression of ATG8 and ATG11 genes significantly reduced the intracellular reactive oxygen species (ROS) content upon H2O2 stress for 6 h, which were 61.23% and 46.35% of the initial state, respectively. Notable, overexpression of ATG8 and ATG11 genes significantly increased the mitochondrial membrane potential (MMP) and ATP content, which were helpful to improve the antioxidant activities of the strains. On the other hand, deletion of ATG8, ATG11 and ATG32 caused mitochondrial damage and significantly decreased cell vitality, and caused the imbalance of intracellular ROS. The intracellular ROS content significantly increased to 174.27%, 128.68%, 200.92% of the initial state, respectively, upon H2O2 stress for 6 h. The results showed that ATG8, ATG11 and ATG32 might be potential targets for regulating the antioxidant properties of yeast, providing a new clue for further research.
Mitophagy/genetics*
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Saccharomyces cerevisiae/genetics*
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Antioxidants
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Hydrogen Peroxide/pharmacology*
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Reactive Oxygen Species
10.Rosmarinic acid inhibits high glucose-induced cardiomyocyte hypertrophy by activating Parkin-mediated mitophagy.
Jiayu DIAO ; Hongmou ZHAO ; Yujie NING ; Wenqi HAN ; Yi WANG ; Gong CHENG ; Xiling SHOU ; Hongjun YOU
Journal of Southern Medical University 2020;40(11):1628-1633
OBJECTIVE:
To evaluate the effect of rosmarinic acid (RA) on mitophagy and hypertrophy of cardiomyocytes exposed to high glucose (HG).
METHODS:
Rat cardiomyocytes (H9c2) exposed to HG (25 mmol/L) were treated with 50 μmol/L RA or with both RA treatment and Parkin siRNA transfection, with the cells cultured in normal glucose (5.5 mmol/L) and HG as the controls. The expressions of PINK1, Parkin and LC3II/LC3I in the cells were detected by Western blotting. The formation of mitochondrial autophagosomes was observed by transmission electron microscope. Flow cytometry was employed to detect the level of reactive oxygen species (ROS) and apoptotic rate of the cells. The activities of respiratory chain complex enzymes were measured by spectrophotometry. Fluorescence enzyme labeling and
RESULTS:
RA treatment significantly increased the expression levels of PINK1, Parkin and LC3-II/I (
CONCLUSIONS
RA can protect rat cardiomyocytes against oxidative stress injury and cardiomyocyte hypertrophy induced by HG by activating Parkin-mediated mitophagy.
Animals
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Cinnamates
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Depsides
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Glucose
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Hypertrophy
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Mitophagy
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Myocytes, Cardiac
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Protein Kinases
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Rats
;
Reactive Oxygen Species
;
Ubiquitin-Protein Ligases/genetics*