No abstract available.
Arthritis, Rheumatoid* ; Humans ; Hyperpigmentation* ; Methotrexate*

Background: Many studies have reported that dog ownership is effective in encouraging physical activity. However, the association between living with a dog and enhanced physical activity has not been clearly verified. Thus, this study aimed to investigate the effects of dog ownership on the amount of physical activity in a representative sample of Korean adults. Methods: This cross-sectional study administered the International Physical Activity Questionnaire (IPAQ) to 1,299 individuals who visited the international healthcare center of one hospital in Seoul, Korea, between August and December in 2018. Based on responses to the IPAQ survey, the amount and duration of physical activity was calculated to analyze relationships with dog ownership. Results: The total amount (P=0.02) and duration of physical activity (P=0.02) and the duration of vigorous-intensity physical activity (P=0.04) were significantly higher among dog owners than non-dog owners. The duration of daily physical activity increased with dog ownership by 18.6 minutes (P=0.01). A comparison of dog owners according to whether they walked with their dogs revealed that dog walking had no significant effect on the amount and duration of physical activity, inactivity, or health-enhancing physical activity status. There was a sex-specific association between dog ownership and total duration of physical activity in females. Conclusion The duration of physical activity increased by dog ownership. However, the higher levels of physical activity among dog owners may not necessarily be explained by dog ownership or walking with dogs. This outcome suggests that raising a dog can help promote physical activity.

Background: This study aimed to examine the relationship between patient experience and medication compliance of patients with dyslipidemia.
Methods: Based on data from the Korea National Health and Nutrition Examination Survey in 2015, the study examined 764 patients treated with dyslipidemia medication. Subjects who responded to the question “Do you currently take medication to lower your blood cholesterol?” with “daily taking” were categorized as the compliant group, and the remaining subjects were classified under the non-compliant group. The patient experience survey included four indicators, in which subjects were divided into groups with a positive and negative patient experience. Data on sociodemographic factors, health-related behaviors, and self-reported comorbid conditions were also collected.
Results: After adjusting the variables, the group with a positive response for the patient experience indicator “doctor spends enough time with the patient during consultation” was 1.89 times more compliant than the group with a negative response (95% confidence interval [CI], 1.03–3.48; P=0.04). For the indicator “doctor provides easy-to-understand explanations,” the group that showed a positive response was 2.74 times more compliant than the group with a negative response (95% CI, 1.39–5.39; P=0.004). For the indicator “doctor involves patients in decisions about care or treatment,” the group that showed a positive response was 2.07 times more compliant than the group with a negative response (95% CI, 1.02–4.22; P=0.04). However, for the indicator “doctor provides the patient a chance to ask questions about treatment,” positive patient experience had no significant association with medication compliance (95% CI, 0.77–2.36; P=0.30).
Conclusion Building a good doctor-patient relationship with positive patient experiences can result in better outcomes for patient care through high medication compliance.

Background: Many studies have reported that dog ownership is effective in encouraging physical activity. However, the association between living with a dog and enhanced physical activity has not been clearly verified. Thus, this study aimed to investigate the effects of dog ownership on the amount of physical activity in a representative sample of Korean adults. Methods: This cross-sectional study administered the International Physical Activity Questionnaire (IPAQ) to 1,299 individuals who visited the international healthcare center of one hospital in Seoul, Korea, between August and December in 2018. Based on responses to the IPAQ survey, the amount and duration of physical activity was calculated to analyze relationships with dog ownership. Results: The total amount (P=0.02) and duration of physical activity (P=0.02) and the duration of vigorous-intensity physical activity (P=0.04) were significantly higher among dog owners than non-dog owners. The duration of daily physical activity increased with dog ownership by 18.6 minutes (P=0.01). A comparison of dog owners according to whether they walked with their dogs revealed that dog walking had no significant effect on the amount and duration of physical activity, inactivity, or health-enhancing physical activity status. There was a sex-specific association between dog ownership and total duration of physical activity in females. Conclusion The duration of physical activity increased by dog ownership. However, the higher levels of physical activity among dog owners may not necessarily be explained by dog ownership or walking with dogs. This outcome suggests that raising a dog can help promote physical activity.

Background: This study aimed to examine the relationship between patient experience and medication compliance of patients with dyslipidemia.
Methods: Based on data from the Korea National Health and Nutrition Examination Survey in 2015, the study examined 764 patients treated with dyslipidemia medication. Subjects who responded to the question “Do you currently take medication to lower your blood cholesterol?” with “daily taking” were categorized as the compliant group, and the remaining subjects were classified under the non-compliant group. The patient experience survey included four indicators, in which subjects were divided into groups with a positive and negative patient experience. Data on sociodemographic factors, health-related behaviors, and self-reported comorbid conditions were also collected.
Results: After adjusting the variables, the group with a positive response for the patient experience indicator “doctor spends enough time with the patient during consultation” was 1.89 times more compliant than the group with a negative response (95% confidence interval [CI], 1.03–3.48; P=0.04). For the indicator “doctor provides easy-to-understand explanations,” the group that showed a positive response was 2.74 times more compliant than the group with a negative response (95% CI, 1.39–5.39; P=0.004). For the indicator “doctor involves patients in decisions about care or treatment,” the group that showed a positive response was 2.07 times more compliant than the group with a negative response (95% CI, 1.02–4.22; P=0.04). However, for the indicator “doctor provides the patient a chance to ask questions about treatment,” positive patient experience had no significant association with medication compliance (95% CI, 0.77–2.36; P=0.30).
Conclusion Building a good doctor-patient relationship with positive patient experiences can result in better outcomes for patient care through high medication compliance.

Atopic dermatitis (AD) is characterized by disturbances in epidermal barrier functions and the hyperactive immune response. Staphylococcus aureus (S. aureus) can be cultured from 90% of AD skin lesions and can exacerbate or contribute to the persistent skin inflammation in AD by secreting toxins with superantigenic properties. Superantigens can induce mast cell (MC) degranulation after penetrating the epidermal barrier. The role of MCs in AD is suggested by the increase in the MC number and MC activation. MCs are activated for degranulation and mediator release by allergens that cross-link IgE molecules or by microbial products. Therefore, MCs may be critically involved in the pathogenesis of AD. However, the understanding mechanisms of MC degranulation by S. aureus in relation to AD have still not been fully elucidated. In this study, we found that live S. aureus or methicillin-resistant S. aureus (MRSA) but not heat-killed bacteria induced MC degranulation. The heat-treatment partially inhibited MC degranulation by conditioned media (CM) of S. aureus or MRSA. The calcium chelator ethylene glycol tetraacetic acid (EGTA) did not block MC degranulation induced by live S. aureus or MRSA, but EGTA-treatment partially inhibited MC degranulation by CM from S. aureus or MRSA. These results suggest that live S. aureus and MRSA can degranulate MCs via direct interaction which may be important role in AD.
Allergens ; Bacteria ; Calcium ; Culture Media, Conditioned ; Dermatitis, Atopic ; Egtazic Acid ; Humans* ; Immunoglobulin E ; Inflammation ; Mast Cells ; Methicillin Resistance ; Methicillin-Resistant Staphylococcus aureus ; Skin ; Staphylococcus aureus ; Superantigens

Atopic dermatitis (AD) is characterized by disturbances in epidermal barrier functions and the hyperactive immune response. Staphylococcus aureus (S. aureus) can be cultured from 90% of AD skin lesions and can exacerbate or contribute to the persistent skin inflammation in AD by secreting toxins with superantigenic properties. Superantigens can induce mast cell (MC) degranulation after penetrating the epidermal barrier. The role of MCs in AD is suggested by the increase in the MC number and MC activation. MCs are activated for degranulation and mediator release by allergens that cross-link IgE molecules or by microbial products. Therefore, MCs may be critically involved in the pathogenesis of AD. However, the understanding mechanisms of MC degranulation by S. aureus in relation to AD have still not been fully elucidated. In this study, we found that live S. aureus or methicillin-resistant S. aureus (MRSA) but not heat-killed bacteria induced MC degranulation. The heat-treatment partially inhibited MC degranulation by conditioned media (CM) of S. aureus or MRSA. The calcium chelator ethylene glycol tetraacetic acid (EGTA) did not block MC degranulation induced by live S. aureus or MRSA, but EGTA-treatment partially inhibited MC degranulation by CM from S. aureus or MRSA. These results suggest that live S. aureus and MRSA can degranulate MCs via direct interaction which may be important role in AD.
Allergens ; Bacteria ; Calcium ; Culture Media, Conditioned ; Dermatitis, Atopic ; Egtazic Acid ; Humans* ; Immunoglobulin E ; Inflammation ; Mast Cells ; Methicillin Resistance ; Methicillin-Resistant Staphylococcus aureus ; Skin ; Staphylococcus aureus ; Superantigens

The striking increase in colorectal cancer (CRC) has shown the great fatality in Korea for more than 15 years. The leading edge of this rising incidence rate is mainly due to the people's dietary changes in Korea. Some studies have reported that the dietary fiber does not have significant cytotoxic effects on CRC cells, which contrasts to the effects of probiotics. It gives a positive evaluation that the nonpathogenic spore-forming Bacillus species among the probiotics including fermented bacteria might have optimistic effects on CRC incidence rate. Recently, we isolated Bacillus lentus (BL) from Korean soybean fermented food. BL showed the cytotoxic effect on human colon carcinoma cell lines HCT116 and SW480. Interestingly, BL did not have effect on human dermal fibroblast cells and human hepatoma cell line HepG2. It suggested that BL has the target cell-specific cytotoxicity toward human colon carcinoma cells. To clarify the death signaling pathway underlying the BL-induced apoptosis in cancer cells, we analyzed the expression of caspases, Bax and Bcl-2 by western blotting. The apoptotic effects by cytotoxic elements were executed by direct BL contact or membrane-derived vesicles isolated from BL. Treatment of HCT116 with BL activated caspase-9, -3 and increased cleavage form of poly (ADP-ribose) polymerase (PARP). However, caspase-8 activity was not increased by BL. BL-activated intrinsic pathway increased the pro-apoptotic Bax, decreased the anti-apoptotic Bcl-2 proteins on mitochondria, disrupted the mitochondrial membrane potential, and then released the cytochrome c from mitochondria. The membrane-derived vesicles (MVs) from BL induced apoptosis of the HCT116. Here, we propose that BL as a strong candidate for the development of apoptosis-specific anti-tumor agent will give great contribution to the understandings of the tumor-microbe interdisciplinary areas.
Apoptosis* ; Bacillus* ; Bacteria ; Blotting, Western ; Carcinoma, Hepatocellular ; Caspase 8 ; Caspase 9 ; Caspases ; Cell Line* ; Colon* ; Colonic Neoplasms* ; Colorectal Neoplasms ; Cytochromes c ; Dietary Fiber ; Fibroblasts ; Humans* ; Incidence ; Korea ; Membrane Potential, Mitochondrial ; Membranes* ; Mitochondria ; Probiotics ; Soybeans ; Strikes, Employee