1.Action of miosis of 1% pilocarpine liposome in rabbits.
Acta Academiae Medicinae Sinicae 2003;25(3):343-345
OBJECTIVETo compare the action of miosis of 1% pilocarpine liposome with 1% pilocarpine solution in rabbits.
METHODS18 white rabbits were randomly divided into 3 groups. Test group received 1% pilocarpine liposome, positive control group received 1% pilocarpine solution, negative control group received liposome. Each eye drop instilled into left eye of rabbits and sterile saline solution instilled into right eye as control. The pupil diameter was measured at time intervals of beginning, 0.25, 0.5, 1, 2, 3, 4, 5, 7 hours.
RESULTSThe mean pupil diameter change of 3 groups in both eyes was not significant (P > 0.05) at beginning. The strongest action of miosis took place 0.25 h in positive control group and 0.5 h in test group after instillation. The dilation of pupil in both groups took place 1 h and 3 h, and the restoration of pupil in both groups took place at 5 h and 7 h. The mean pupil diameter of negative control group was not significant in seven hours.
CONCLUSIONSThe results suggest that 1% pilocarpine liposome improves the bioavailability and prolong the duration of its action.
Animals ; Delayed-Action Preparations ; Female ; Liposomes ; pharmacology ; Male ; Miotics ; pharmacology ; Pilocarpine ; administration & dosage ; pharmacology ; Pupil ; drug effects ; Rabbits ; Random Allocation
2.Differential Expression of Activating Transcription Factor-2 and c-Jun in the Immature and Adult Rat Hippocampus Following Lithium-Pilocarpine Induced Status Epilepticus.
Si Ryung HAN ; Cheolsu SHIN ; Seongkyung PARK ; Seonyoung RHYU ; Jeongwook PARK ; Yeong In KIM
Yonsei Medical Journal 2009;50(2):200-205
PURPOSE: Lithium-pilocarpine induced status epilepticus (LPSE) causes selective and age-dependent neuronal death, although the mechanism of maturation-related injury has not yet been clarified. The activating transcription factor-2 (ATF-2) protein is essential for the normal development of mammalian brain and is activated by c-Jun N-terminal kinase (JNK). It induces the expression of the c-jun gene and modulates the function of the c-Jun protein, a mediator of neuronal death and survival. Therefore, we investigated the expression of c-Jun and ATF-2 protein in the immature and adult rat hippocampus to understand their roles in LPSE-induced neuronal death. MATERIALS AND METHODS: Lithium chloride was administrated to P10 and adult rats followed by pilocarpine. Neuronal injury was assessed by silver and cresyl violet staining, performed 72 hours after status epilepticus. For evaluation of the expression of ATF-2 and c-Jun by immunohistochemical method and Western blot, animals were sacrificed at 0, 4, 24, and 72 hours after the initiation of seizure. RESULTS: Neuronal injury and expression of c-Jun were maturation-dependently increased by LPSE, whereas ATF-2 immunoreactivity decreased in the mature brain. Since both c-Jun and ATF-2 are activated by JNK, and targets and competitors in the same signal transduction cascade, we could speculate that ATF-2 may compete with c-Jun for JNK phosphorylation. CONCLUSION: The results suggested a neuroprotective role of ATF-2 in this maturation-related evolution of neuronal cell death from status epilepticus.
Activating Transcription Factor 2/*metabolism
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Animals
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Antimanic Agents/pharmacology
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Blotting, Western
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Hippocampus/drug effects/*metabolism
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Immunohistochemistry
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Lithium/pharmacology
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Miotics/pharmacology
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Pilocarpine/pharmacology
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Proto-Oncogene Proteins c-jun/*metabolism
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Rats
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Status Epilepticus/*chemically induced