Objective To document the HEV seroprevalence in this region,we tested HEVIgG for part of outpatients ' serosample.Methods We collected 415 serosample in outpatients of the hospital and tested HEV IgG anti-body with a commercial detection kit produced by Wantaicompany.The results were examined some different group by using chi-square testing.Results The HEV positive rate of 415 serosamples was 21.93％.It was significant difference between genders and two age groups.And it was not significant difference in urban-rural.Conclusion The HEV positive rate of our city was the average of overall HEV seroprevalence in China.In adults,HEV positive rate was significant higher to children.The effective vaccination in children could controlled the HEV prevalence.

Objective: To prepare peptide minotope-based recombinant diagnostic antigen of Epstein-Barr virus (EBV) infection and evaluate its antigenicity preliminarily. Methods: With Trx at the N-terminal and His tag at the C-terminal, the peptide minotope of EBV (GP125, F1, A2, A3C2) was expressed in Escherichia coli and purified by affinity and anion exchange chromatography (designated 'H58’); based on antigenicity of H58 identified by Western blotting (WB), we constructed and evaluated a novel early diagnostic ELISA for EBV infection. Results: The soluble H58 protein with high concentration (2.8 mg/ml) and purity (99.01) was obtained; WB analysis found that there was an obvious band (28 ×10³) on the NC membrane, using H58 anti-Trx monoclonal antibody or acute-phase sera of EBV infection as the first antibody. With the novel ELISA, 50 positive sera of EBV infection and 50 negative sera were detected, displaying that the grouping of OD value of positive serum (95%CI: 1.233-1.489) and negative serum (95%CI: 0.113-0.159) was different (P<0.05) with the sensitivity 98.0%, specificity 96.0% and kappa value 0.940. Conclusions By E. coli expression and affinity and ion exchange chromatography purification, the peptide minotope-based recombinant diagnostic antigen of EBV infection was obtained with excellent antigenicity, which could be applied for serological detection of EBV infection.