1.Effects of mineral trioxide aggregate on the proliferation and differentiation of human pulp cells from primary and permanent teeth.
Minyong WANG ; He LIU ; Shenglin LI ; Man QIN
West China Journal of Stomatology 2015;33(1):75-79
OBJECTIVEThis study aims to investigate the effects of mineral trioxide aggregate (MTA) and calcium hydroxide on proliferation and differentiation of human pulp cells from primary and permanent teeth.
METHODSCell proliferation was detected by methyl thiazolyl tetrazolium (MTT) assay. The mRNA expression levels of dentinogenesis-related factors, including alkaline phosphatase (ALP) and dentin sialophosphoprotein (DSPP), and odontoclastogenesis-related factors, such as osteo- protegerin (OPG) and receptor activator of NF-κB ligand (RANKL), were determined by real time polymerase chain reac- tion (PCR).
RESULTSPrimary and permanent pulp cells treated with calcium hydroxide exhibited significantly lower proli- feration rates than the control cells (P<0.01). By contrast, the MTA-treated group showed significantly higher proliferation rates than the control group (P<0.01). Real time PCR results showed that calcium hydroxide-treated primary pulp cells exhi- bited significantly decreased ALP, DSPP, and OPG expression compared with the control group (P<0.01). Conversely, the MTA-treated group displayed significantly increased ALP, DSPP, and OPG expression (P<0.01). Calcium hydroxide-treated primary pulp cells also exhibited significantly upregulated RANKL expression (P < 0.01); by contrast, MTA-treated cells did not show any change in RANKL expression (P>0.05). Likewise, MTA-treated permanent pulp cells showed significantly upregulated ALP and DSPP expression (P < 0.01). However, the calcium hydroxide-treated group remained almost the same as the control group (P > 0.05). Neither MTA nor calcium hydroxide affected OPG and RANKL expression in per- manent pulp cells (P > 0.05).
CONCLUSIONMTA is more suitable as a pulp-capping agent, particularly in primary teeth, than calcium hydroxide.
Aluminum Compounds ; Calcium Compounds ; Calcium Hydroxide ; Cell Differentiation ; Cell Proliferation ; Dental Pulp ; Dentition, Permanent ; Drug Combinations ; Extracellular Matrix Proteins ; Humans ; Oxides ; Phosphoproteins ; Sialoglycoproteins ; Silicates
2.Experimental research in differences between two methods of holding the syringe to draw physic liquors
Lingling CHEN ; Huizhi ZHAN ; Ping ZHOU ; Minyong LIU
Chinese Journal of Practical Nursing 2012;28(4):58-60
Objective To explore the differences of bacterial culture results and time consumption between the two methods of holding the syringes to draw physic liquors,so as to find the objective and scientific evidences to raise the efficiency of the clinical nursing work and to ensure the medication safety at the same time. Methods Making bacterial cultures for the physic liquors respectively drawn by grasping the piston of syringes (30 cases in the experimental group) and grasping the piston holder of syringes(30cases in the control group).Recorded the time consumption by grasping the piston of syringes to draw the physic liquors (the experimental group) and grasping the piston holder of syringes to draw the physic liquors (the control group).The testing results were compared between the two groups. Results Bacterial cultures:There was no bacteria growth in both groups.Timing:The time spent by grasping the piston of syringes to draw physic liquors was obviously shorter than that by grasping the piston holder of syninges to draw physic liquors. Conclusions Grasping the piston of syringes to draw physic liquors can ensure the medication safety and raise the work efficiency.
3.Efficacy of probiotics on chemotherapy-induced intestinal mucositis in rats: a meta-analysis
Yujia HUANG ; Liangnan ZENG ; Ruichen LIANG ; Xianhe WU ; Minyong LIU ; Changmei YANG
Chongqing Medicine 2017;46(25):3560-3564
Objective To evaluate the efficacy of probiotics on chemotherapy-induced intestinal mucositis in rats.Methods The databases including PubMed,EMbase,Cochrane,CBM,CNKI,WanFang and VIP were retrieved from their establishment to April 2016.The related randomized controlled trials(RCT) on the effects of probiotics for treating chemotherapy-induced intestinal mocositis in rats were included.The relevant literatures were screened according to the inclusion and exclusion criteria,then the data were extracted and analyzed.Results Total 6 RCT were included.Compared with the control group,the intestinal secretion and absorption function in the probiotics group was strengtnened[SMD=1.73,95 %CI(0.79,2.68),P<0.01],jejunal anti-oxidant capacity was increased [SMD=-2.12,95%CI(-3.56,-0.67),P<0.01],however low dose probiotics (<1 × 109 cfu/d)had no protective effect on small intestine[SMDjejunum =-0.06,95%CI(-0.51,0.40),SMDileum =0.02,95% CI(-0.71,0.75);P >0.05],while high dose probiotics(≥ 1 × 109 cfu/d) could reduce the intestinal pathological damage[SMDjejunum =-0.64,95 % CI (-1.20,-0.09),SMDileum=-0.85,95% CI(-1.59,-0.12);P<0.05].Conclusion High dose probiotics can reduce chemotherapy-induced intestinal mucositis in rats.Because of less included literatures and the influence of publication bias,the effect of probiotics on chemotherapy induced mucositis could be overestimated.
4.Application prospect of computer vision technology assisted ultrasonography in diagnosis and treatment of liver diseases
Guoxu FANG ; Wenting XIE ; Kaizhi CHEN ; Siqi CHEN ; Minyong CHEN ; Xiangwen LIAO ; Lina TANG ; Jingfeng LIU
Chinese Journal of Digestive Surgery 2023;22(4):462-467
Ultrasound examination has the advantages of non-radiation, non-invasive, low cost and high efficiency, and is the most commonly used method of liver imaging examination. In recent years, the application of computer vision technology to the intelligent analysis of ultrasound images has become a research hotspot in the field of intelligent healthcare. Through large-scale data training, the intelligent analysis model of ultrasound omics based on machine learning algorithm can assist clinical diagnosis and therapy, and improve the efficiency and accuracy of diagnosis. Based on the literature, the authors summarize the application proprect of computer vision technology assisted ultrasonography in the evaluation of diffuse liver lesions, focal liver lesions, microvascular invasion of liver cancer, postoperative recurrence of liver cancer, and postoperative therapy response to trans-catheter arterial chemoembolization.
5. Immunogenicity analysis of sequential inoculation of different strains of poliomyelitis vaccines in Shanghai
Zhuoying HUANG ; Xiaodong SUN ; Minyong LIU ; Chongshan LI ; Jia REN ; Jiayu HU ; Jianping YANG ; Jiechen LIU ; Zhi LI ; Yuying YANG ; Yunyi LI ; Jing LU
Chinese Journal of Preventive Medicine 2019;53(5):513-518
Objective:
To evaluate the immunogenicity of different strains of inactivated poliomyelitis vaccines (IPV) by sequential program.
Methods:
This parallel-group controlled trial was conducted in immunization clinics in Shanghai from March 2016 to September 2017. Sabin strains inactivated poliomyelitis vaccines (sIPV), WPV strains inactivated poliomyelitis vaccines (wIPV) and live poliomyelitis Type Ⅰ Type Ⅲ vaccine (bOPV) as the investigational vaccine were used at 2, 3, 4 months old in 325 infants in Shanghai. Infants vaccinated by four sequential program were divided into 4 groups: sIPV+sIPV+bOPV, sIPV+wIPV+bOPV, wIPV+sIPV+bOPV and wIPV+wIPV+bOPV. A total of 230 investigators′ blood samples were collected before primary immunization and 163 investigators′ blood samples were collected after primary immunization. A total of 151 investigators (36, 44, 30 and 41 in each group) finished primary immunization and blood sampling before and after the primary immunization. The geometric mean titer (GMT) of poliovirus typesⅠ and Ⅲ neutralizing antibody was tested and calculated, and the positive results of antibody before and after primary immunization were analyzed.
Results:
Among the 151 investigators, the age were (2.27±0.61) months and birth weight were (3.27±0.43) kg, and 70 were male. The positive rates of typeⅠwas 98.68% (149 cases), and type Ⅲ was 97.35% (147 cases); the number of investigators tested in each group was 36, 44, 30 and 41, respectively; the positive rates of typeⅠwas 97.22% (35 cases), 100.00% (44 cases), 96.67% (29 cases) and 100.00% (41 cases) (