Long-term potentiation (LTP) of synaptic transmission between the dendrites of the pyramidal cells of CA[ region and the Schaffer-commissu-ral pathway was induced with electric stimulation in the isolated hippocampal slices of rats.The effects of 3 convulsants,soman,kianic acid.and picroto-xin,on the induction of LTP in this region were observed.It was found that a short series of electric impulses could effectively induce LTP in most of the slices of the hippocampus and the amplitude of the population spikes showed a similar change as the field excitatory postsynaptic potential did in the rising phase.Picrotoxin could facilitate the induction of LTP when it evoked an epileptoform electric activity in the slices,kianic acid could also evoke an epileptoform activity but significantly blocked the induction of LTP,and soman could not evoke obvious epileptoform activity but blocked the induction of LTP.The above findings suggest that a short series of electric impulses can effectively induce LTP between the pyramidal cells and the Schaffer-commisural pathway in the CA1 region,the effects of epileptoform activity on the induction of LTP depends on the mechanism to evoke the epileptoform activity,and the effects of soman on the induction of field potential may involve the noncholi-nergic system in the synaptic connections.

Objective To investigate the effects of midazolam, ketamine and propofol on spatial cognitive function and the mRNA expression of N-methyl-D-aspartate (NMDA) receptor subunits NR1 and NR2B in the hippocampus of aged rats. Methods Thirty-two Wistar rats of both sexes aged 18 months were randomly divided into 4 groups: Ⅰ control group (n = 9); Ⅱ midazolam group (M, n = 8);Ⅲ propofol group (P, n = 7) and Ⅳ ketamine group (K, n = 8). The animals received intraperitoneal (IP) midazolam 30 mg?kg-1 or propofol 60 mg ?kg-1 or ketamine 80 mg?kg-1 once a day for 3 days whereas the animals in control group received IP normal saline 2 ml instead. One day after the last drug administration the animals underwent Morris water maze test 4 times a day for 3 consecutive days. The animals were killed at 1h after last test and the brains were immediately removed for determination of NR1 and NR2B mRNA expression in the hippocampus using RT-PCR.Results The latency period and swimming distance were significantly shorter on the 3rd day of water maze test than on the 1st day in control group and group K, P. The latency period on the 1st day was significantly longer in group K than in control group. The NR1 mRNA expression in group M was significantly higher while the NR2B mRNA expression in group K and P was significantly lower than that in control group (P

Objective To assess the effects of norephrine and dopamine on renal blood and renal function in a dog model of endotoxin shock. Methods Twenty-one mongrel dogs (weight 14-30 kg) were anesthetized with pentobarbital sodium and intubated. Spontaneous breathing was maintained. Swan-Ganz catheter was inserted via right external jugular vein. A 18G cannula was inserted via vein of the left upper limb for infusion of fluid and a 20G cannula in femoral artery for blood sampling and monitoring of arterial pressure. Abdomen was opened and right renal artery was exposed. A electromagnetic flowmeter (4mmφin interval diameter) was placed around the renal artery for measurement of renal blood flow. Shock was induced with intravenous administration of endotoxin 2mg/kg (LPS O55B5 Sigma). One hour after iv injection of LPS, the animals were divided into 3 groups with 7 dogs each: group Ⅰ (group NE) received norepinephrine infusion 40ng@ kg-1@ min-1 for 1h; group Ⅱ (group DA) received dopamine infusion 4μg.kg-1 @min-1 for 1h; group Ⅲ (group NE + DA) received NE 40 ng@ kg-1 @min-1 and 4 μg@ kg-1@min-1 for 1h. MAP, HR, cardiac output(CO), stroke volume index(SVI), CVP, PCVP, SVR, renal blood flow and renal function (serum Na+ , K+ , creatinine, urea nitrogen and uric acid ) were measured before LPS administration, lh after administration of LPS, at the end of norepinephrine and/or dopamine infusion, 1 and 2h after NE and/or DA infusion. Results MAP, CO and renal blood flow decreased significantly after LPS administration, but there was no significant change in renal function after LPS was given. After infusion of norepinephrine and/or dopamine MAP, SVI and CO increased significantly but there was little change in renal blood flow and renal function. Conclusions Low dose norepinephrine can improve hemodynamics and maintain renal blood flow and renal function especially when combined with dopamine during endotoxin shock in dogs.

Objective To investigate the effects of repeated inhalation of sevoflurane on cognitive function in aged rats. Methods Twenty-four Wistar rats of beth sexes aged 18 months weighing 270-450 g were randomly divided into 3 groups(n=8 each):control group(C),2%aevoflurane group(S1)and 3% sevoflurane group (S2).Group C inhaled oxygen(2.5 L/min)and nitrogen(6L/min)only, while group S1 and S2 inhaled 2%and 3%sevonurane in oxygen(2.5 L/min)and nitrogen(6 L/min)100 min/d for 5 d respecfivelv. The Morris watemaze test was performed once a day for 6 consecutive clays after the last inhalation(T1-6).The swimming time and distance at T1-5 and probe time and swimming distance in the fourth quadrant at T6 were recorded. The aninlala were killed within 60 min after the last test and the hippecampus were immediately removed for determination ofthe expression of NMDAR, NRI and NR2B mRNA using RT-PCR.Results The swimming time and disl .ance were significantly longer at T1,and the probe time in the fourth quadrant shorter and expression of NMDAR mRNA hisher at T6 in group s2 than in group C(P<0.05).Conclusion Repeated inhalation of 2% sevoflurane can not induce cognitive disordel-,while 3%sevoflurane Call induce transient cognitive decline in aged rats.

Objective To evaluate the value of breast molybdenum target radiography in evaluating the enlarged axillary lymph nodes in breast diseases.Methods 79 patients underwent breast molybdenum target radiography,including conventional laxial and oblique projection. Some of them were examine furtheremore in compress projection in axillary fossa. The X-ray findings were analysis. And radiologic-pathologic correlation were carried out.Results There were 46 cases of brease carcinoma, 20 cases of hyperplasia,7cases of fibroadenoma,2 cases of plasmacytic mammitis,2cases of lipoma and 2 cases of papilloma in 79 patients. In the 33 cases of breast benign lesion , the enlarged axillary lymph nodes were seen bilaterally in 12 cases, sum was 1 to 5 in each case, smaller than 2 cm in diameter in 31 cases and uneven density in 13 cases were showed. In the 46 cases of brease carcinoma, the enlarged lymph nodes were seen laterally in 43cases, sum was 1 to 4 in each cases, larger than 2 cm in diameter was showed in 17 cases, and all were even density. There were statistical differences in number, diameter and density of enlarged lymph nodes between two groups. Conclusion The enlarged axillary lymph nodes are of different X-ray features in breast benign lesion and malignant lesion,it is helpful in differential diagnosis of breast diseases.

Objective To study the value of double contrast barium enema(DCBE),colonoscopy and spiral CT in examination of colonic disease.Methods 325 cases with colonic disease underwent DCBE,colonoscope and CT examinations.The detecting rates and the accurate rates in localizing the lesions were compared among these three examinations.Results The detecting rate of lesions by DCBE,colonoscope and CT were 91.1,89.8 and 64.6,respectively,there was no statistical difference between the former two,but there was difference between the former and CT(P

Objective To study the effect of Alemtuzumab on acute rejection (AR) and graft survival after kidney transplantation.Method Published domestic and foreign literatures regarding the effects of Alemtuzumab used on acute rejection and graft survival were reviewed,and Meta analysis was employed to analyze the Results.Odds ratio (OR) and its 95％ confidence interval (95％ CI) were used as the parameters to evaluate the therapeutic effects.The statistical analyses were performed with RevMan 5.1 software.Result A total of 9 pertinent research articles were reviewed.Meta-analysis of pooled results indicated that Alemtuzumab prevented the recipients of kidney transplantation from acute rejection effectively with half year prevention of OR 0.37 and 95％ CI 0.24-0.58 (P＜0.01),one year prevention of OR 0.43 and 95 ％ CI 0.29-0.64 (P＜0.01),and two year prevention of OR 0.69 and 95％ CI 0.47-1.02 (P ＜ 0.01),respectively.It was revealed that Alemtuzumab could reduce the incidence of acute rejection by 55％ in half year,51％ in one year and 28％ in two years,respectively.No statistically significant difference in graft survival was found between Alemtuzumab group and control group (OR =1.18,95％ CI 0.76-1.85,P =0.46).No statistically significant difference in patients' survival was found between Alemtuzumab group and control group (OR =0.94,95 ％ CI 0.52-1.72,P =0.85).Conclusion Alemtuzumab may effectively prevent the recipients of kidney transplantation from acute rejection,but not obviously influence the graft and patient survival.

The aim of the present study was to detect the transmission rate of ultrasonic low intensity pulsed ultrasound (LIPUS) through polytetrafluoroethylene (PTFE) membrane (Thickness: 0.01 mm) and Bio-Gide collagen membrane, and to provide the basis for the barrier membrane selection on the study of LIPUS combined with guided tissue regeneration (GTR). The ultrasonic (LIPUS, frequency 1.5 MHz, pulse width 200 micros, repetition rate 1.0 kHz) transmission coefficient of the two kinds of barrier membrane were detected respectively through setting ten groups from 10 to 100mW/cm2 every other 10 mW/cm2. We found in the study that the ultrasonic transmission coefficient through 0.01 mm PTFE membrane was 78.1% to 92.%, and the ultrasonic transmission coefficient through Bio-Gide collagen membrane was 43.9% to 55.8%. The ultrasonic transmission coefficient through PTFE membrane was obviously higher than that through Bio-Gide collagen membrane. The transmission coefficient of the same barrier membrane of the ultrasonic ion was statistically different under different powers (P < 0.05). The results showed that the ultrasonic transmittance rates through both the 0.01 mm PTFE membrane and Bio-Gide collagen membrane were relatively high. We should select barrier membranes based on different experimental needs, and exercise ultrasonic transmission coefficient experiments to ensure effective power.
Biocompatible Materials ; Collagen ; chemistry ; Membranes, Artificial ; Permeability ; Polytetrafluoroethylene ; chemistry ; Ultrasonics

Objective To analyze the mammographic features and pathological characteristics among different molecular subtypes of breast cancer.Methods The results of 210 cases breast cancer identified by postoperative pathology were collected and classified to three groups;Luminal,HER-2(+)and TNBC(triple negative breast cancer)by molecular subtypes.Three groups'mammograph-ic features and pathological characteristics were compared.Results 210 cases included 147 Luminal cases,30 HER-2 (+)cases and 33 TNBC cases.There were statistically significant difference between tumor grading and lymph node metastasis (P <0.05).Three groups had statistically significant difference among mass number,mass margin and calcification incidence(P <0.05),and had no sta-tistically significant difference between mass size and shape(P >0.05).The mammographic features of Luminal molecules subtypes showed more mass with burr,HER-2(+)molecules subtypes showed no fixed features but more calcification incidence than other groups,TNBC molecules subtypes showed merely mass with clear margin and less calcification.Conclusion The mammographic fea-tures and pathological characteristics of different molecular subtypes of breast cancer are significant differences.

Objective The purpose of this study was to assess the technical feasibility, safety and effectiveness of percutaneous transluminal angioplasty (PTA)for the treatment of severe limb ischemia caused by tibioperoneal arteries occlusion. Methods From June 2004 to May 2007,35 patients with critical limb ischemia (CLI)were treated on an intention-to-treat basis with tibioperoneal arterial PTA. Main outcome measures were technical success rate, primary patency rate, limb salvage rate and complications. Results The technical success rate of tibioperoneal arterial PTA Was 83%.Mean follow-up time was 11.5 months, primary patency rate and limb salvage rate were 57%and 82%,respectively.Mean length of tibioperoneal arterial PTA was 9.5 cm(4.5～14 cm),concurrent treatment of iliac artery or femoral-popliteal artery was carried out in 19 patients via either angioplasty or combined with stenting.There were three complications including arterial spasm and thrombosis in one, which Was relieved by thrombolysis and antispasmodics, and artery perforation in 2 cases, which was treated conservatively. Conclusion In tibioperoneal arterial occlusions in CLI patients, PTA is feasible with a high initial technical success rate and high limb salrage rate,tibioperoneal arterial PTA carries a lower morbidity and less severe complications. PTA is a safe, effective and good treatment alternative for CLI patients of tibioperoneal arterial occlusions.