OBJECTIVETo investigate the monoterpene glycosides in Paeonia lactflora by UPLC-MS/MS.

METHODAn Acquity UPLC BEH C18 column (2.1 mm x 50 mm) with 1.7 microm particle size was used. The mobile phase was composed of acetonitrile and 0.1% formic acid in gradient mode. The flow rate was 0.4 mL x min and the chromatographic run time was 9 min for one run. The mass spectrometer equipped with an eletrospray ion source in negative ion mode.

RESULTSTotally six glycosides were analyzed and identified by the established UPLC-MS/MS method.

CONCLUSIONThe method was rapid, sensitive, and extremely useful for rapid identification of glycosides in P. lactiflora.

Molecular Structure ; Paeonia ; chemistry ; Reproducibility of Results ; Spectrometry, Mass, Electrospray Ionization ; methods ; Tandem Mass Spectrometry ; methods

Objective To study the correlation between intermedin 1-53 (IMD 1-53) with hypertension and formation of carotid atheromatous plaques.Methods One hundred and thirty-eight patients with 1-2 degree hypertension admitted to our hospital for carotid ultrasonography were divided into atheromatous plaques group (n =98) and atheromatous plaques-free group (n=40) with 30 subjects undergoing physical examination served as a control group in this study.Their serum IMD 1-53 level,blood pressure,and carotid IMT were measured and compared.Results The SBP [(158.57±13.55)mm Hg and (145.16±14.54)mm Hg vs (125.24±10.64)mm Hg,1 mm Hg=0.133 kPa],DBP[(95.23±5.62)mm Hg and (87.15±4.72)mm Hg vs (80.31±4.62)mm Hg] and carotid IMT[(1.26±0.38)mm and (1.05±0.28)mm vs (0.87±0.38)mm] and serum IMD 1-53 level (7.20±1.62 ng/L vs 5.05±1.85 ng/L,P＜0.01) were significantly higher in atheromatous plaques group and atheromatous plaques-free group than in control group and in atheromatous plaques group than in atheromatous plaques-free group.Conclusion IMD 1-53 is involved in the formation of carotid atheromatous plaques and can resist or delay the formation of carotid atheromatous plaques.