Objective To observe the influencing of Yiqi Huayu Jiedu Prescription on the growth of HepG2 nude mice transplantation tumor and the expression of related factors of vascular mimicry. Methods Models of transplanted tumors, which were made by HepG2 cells in nude mice, were randomly divided into 7 groups, Yiqi Huayu Jiedu Prescription group, Astragali Radix group, Curcumae Rhizoma group, Paridis Rhizoma group, Gecko group, cis-platinum group, and model group. Except for the model group, the rest groups were given relevant medicine for intervention. 21 days later. HIF-1α, MMP-2, MMP-9, and E-cad were detected by immunohistochemistry, and Twist1 and Bcl-2 were detected by fluorescence quantitative PCR. Results Compared with the model group, tumor volume in the rest groups decreased (P<0.05), and the effect in the Yiqi Huayu Jiedu Prescription group was more obvious than the Astragali Radix group, Paridis Rhizoma group and Gecko group (P<0.05);The expression of vasculogenic mimicry structure was rare in each group, and the model group and cis-platinum group were the most obvious;Except for the Astragali Radix group, the expressions of HIF-1α, MMP-2, and MMP-9 showed statistical significance compared with model group (P<0.05);The expression of E-cad in the Yiqi Huayu Jiedu Prescription group and Astragali Radix group showed statistical significance (P<0.05);The expression of Bcl-2 in the Yiqi Huayu Jiedu prescription group, Paridis Rhizoma group, and Gecko group decreased significantly compared with the model group (P<0.05);The expression of Bcl-2 in the Yiqi Huayu Jiedu prescription group was much better than the other groups (P<0.05);The expression of Twist1 showed statistical significance in the Yiqi Huayu Jiedu Prescription group, Curcumae Rhizoma group, Paridis Rhizoma group, and cis-platinum group (P<0.05). Conclusion Yiqi Huayu Jiedu Prescription can reduce expression of HIF-1α, Twist1, Bcl-2, MMP-2, and MMP-9, and increase expression of E-cad, thereby inhibiting the formation of vascular mimicry.

Objective To investigate the reasons of medication errors in clinical nursing , so as to explore corresponding measure to ensure medication safety .Methods Reported medication errors from 41 nursing units in 2011 and problems discovered from drug safety special inspection in November were analyzed , and safety manual for nursing medication was designed and listed in tabular form , which included perfecting a variety of drug safety management organizations and systems , collecting clinical multi-product, multi-gauge, seemingly and sounding drugs into a picture for comparison , clinical control of drip rate and drugs prone to sickness, drug preparation information, drug incompatibility, drug favorable for intravenous infusion , emergency handling of high-risk drug extravasation , pharmacological knowledge of rescue medication and so on . Medication procedure , organization of training and assessment were also regulated by a series of measures . Results Medication errors in 2012 were 15 cases less than those in 2011, with the rate of 18.5%.Compared with 2011 , drug safety management was strengthened and nurses ’ drug management skills , medication knowledge as well as ability were improved by the production and application of safety manual for nursing medication in 2012 to ensure patients ’ medication safety .Conclusions The safety manual can provide reference for clinical nursing medication, guide clinical medication and effectively improve nurses’ medication ability.