We applied two classical algorithms in hyperspectral imaging to extract endmember for biological fluorescence imaging. A combined algorithm was found to initialize the PPI with decreasing the number of multi-spectral pixels, with subsequent N-FINDR refinement, which could make a better result.
Algorithms ; Biology ; methods ; Diagnostic Imaging ; methods ; Image Interpretation, Computer-Assisted ; methods ; Microscopy, Fluorescence ; methods ; Spectrometry, Fluorescence ; methods