1.Effects of timosaponin B-II on differentiation of rat neural stem cells into tyrosine hydroxylase posi-tive neurons in vitro
Minna ZHANG ; Tingting WANG ; Ming ZHONG ; Simin WANG ; Wenxia DENG ; Guanghui WANG ; Gong-Pu ZHENG ; Caiju ZHOU ; Chenggang HUANG
Chinese Journal of Behavioral Medicine and Brain Science 2019;28(8):740-745
Objective To explore the effect of timosaponin B-II ( TB-II) on the differentiation of neural stem cells (NSCs) into tyrosine hydroxylase (TH) positive neurons in neonatal rats. Methods The biological functions of self-proliferation and multi-differentiation of NSCs were identified by primary culture, cell proliferation counting,morphological observation and immunology. NSCs of SD rats were cultured in vitro and treated with different concentrations of TB-II (10 μg/ml,30 μg/ml ,100 μg/ml) for 7 days. Immuno-histochemistry was used to detect the effect of TB-II on the differentiation of NSCs into TH-positive neurons, and Western blot was used to detect the expression of TH protein in neurons. Results ( 1) The cultured cells had the ability to self-proliferation,expressed nestin protein and differentiated into neurons and glial cells. So the cultured cells were conformed to the biological function of neural stem cells. (2)Compared with the control group,the TH positive cell ratio of TB-II 30 μg/ml group and TB-II 100 μg/ml group increased ((10. 03± 1. 36)%),( 20. 01± 3. 37)%),(31. 32± 3. 98)%) ,the difference was significant ( t=6. 15, 16. 54,both P<0. 05). There was no significant difference between TB-II 10 μg/ml group and control group (P>0. 05). (3)Western results showed that the relative expression of TH protein in TB-II 30 g/ml group and TB-II 100 μg/ml group was higher than that in control group,the difference was statistically significant (con-trol group: (1. 02±0. 24),TB-II 30μg/ml group: (3. 64±1. 78),TB-II 100 μg/ml group: (5. 88±2. 34);t=12. 58,9. 15,both P<0. 05). There was no significant difference between TB-II 10 μg/ml group and con-trol group (P>0. 05). Conclusion TB-II can promote the differentiation of NSCs into TH-positive neurons.
2. The characteristics of recent HIV-1 infection and associated factors in Tianjin
Tielin NING ; Yan GUO ; Minna ZHENG ; Jianyun BAI ; Xuan ZHAO ; Ning ZHOU ; Hui GONG ; Shaohui CHENG
Chinese Journal of Preventive Medicine 2019;53(3):323-326
The newly reported HIV infected cases was collected, and HIV blood samples were detected to identify recent HIV infection in Tianjin during 2008-2015. Factors associated with HIV-1 infection were analyzed by the univariate and multivariate unconditional logistic regression. The recent HIV-1 infection proportion of homosexuals increased from 37.70% in 2008 to 83.68% in 2015. Those cases who aged ≤30 years (
3.Pathologic characteristics of bone marrow for CD5 positive small B cell lymphoma.
Shusong PENG ; Fuqiang ZHU ; Sijing YOU ; Minna GONG ; Zhonglian WEN
Journal of Central South University(Medical Sciences) 2016;41(4):374-379
OBJECTIVE:
To study the pathologic characteristics of bone marrow for CD5 positive small B cell lymphoma (SBL).
METHODS:
The pathologic profiles of 92 patients with CD5 positive SBL were retrospectively analyzed. The morphologic and immunophenotypic features were analyzed by flow cytometry and immunohistochemistry. IgH/CCND1 was examined by fluorescence in situ hybridization (FISH).
RESULTS:
A total of 92 patients with CD5 positive SBL were enrolled in this study, including 56 (60.9%) chronic lymphocytic leukemia /small lymphocytic lymphoma (CLL/SLL), 23 (25.0%) mantle cell lymphoma (MCL) and 13 other SBL (14.1%). Among the 13 other cases, 5, 4 and 4 cases were follicular lymphoma (FL), lymphoplasmacytic lymphoma (LPL) and splenic marginal zone lymphoma (SMZL), respectively. The frequency of patterns for bone marrow infiltration was as follow: diffuse pattern (19/92), mixed pattern (15/92), nodular pattern (9/92), interstitial pattern (8/92), and intrasinusodial pattern (2/92). All patients expressed CD19, CD20 and CD5. According to the immunophenotypic score system, all the CLL patients had 4-5 scores, while SMCL and other SBL patients had less than 3 scores. For the other SBL patients, 5 FL expressed CD10, while 3 FL, 1 LPL and 3 SMZL expressed CD23. There was a significant difference in the expression of CD23, sIgM, FMC7, CD11C and CD22 between the CLL and MCL groups (P<0.01). All 23 MCL patients expressed cyclin D1 and showed IgH/CCND1 gene translocation by FISH detection.
CONCLUSION
CD5 positive SBL includes a variety of types of lymphoma. Patterns of bone marrow for CD5 positive SBL are diversity. Immunophenotypic analysis by flow cytometry is essential in the diagnosis and differential diagnosis of CD5 positive SBL, especially for CLL.
Bone Marrow
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pathology
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CD5 Antigens
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metabolism
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Diagnosis, Differential
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Flow Cytometry
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Humans
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Immunohistochemistry
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In Situ Hybridization, Fluorescence
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Leukemia, Lymphocytic, Chronic, B-Cell
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diagnosis
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Lymphoma, B-Cell
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diagnosis
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Lymphoma, Follicular
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diagnosis
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Lymphoma, Mantle-Cell
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diagnosis
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Oncogene Proteins, Fusion
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metabolism
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Retrospective Studies
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Splenic Neoplasms
;
diagnosis
4. Evaluation of a novel HIV-1 DNA reagent in clinical application
Long LI ; Cong DU ; Fangning ZHAO ; Bin XU ; Minna ZHENG ; Zongna LI ; Ning ZHOU ; Hui GONG ; Yan GUO ; Shaohui CHENG ; Maohe YU
Chinese Journal of Experimental and Clinical Virology 2018;32(6):646-649
Objective:
To evaluate the clinical application of a novel HIV-1 DNA reagent.
Methods:
HIV-1-infected and non-infected human blood samples were selected, as well as weakly positive samples, indeterminate samples, specific samples. Compared the result of HIV-1 DNA reagent with HIV-1 infection status (refer to the National Guideline for Detection of HIV/AIDS (2015)), the accuracy of the HIV-1 DNA reagent was evaluated in clinical application; Meanwhile, the commercially available RNA quantification kit was selected as reference reagent for parallel detection, and then the consistency and differences were evaluated between HIV-1 DNA reagent and RNA quantification reagent.
Results:
A total of 95 whole blood samples were tested by the HIV-1 DNA reagent. Taking the HIV-1 infection status as the reference standard, the result showed that the positive agreement rate was 100% (95%