OBJECTIVETo study the antitumor effects of total-flavonoid from S. chamaejasmel.

METHODThe in vitro antitumor activity against human cancer cell lines, such as stomach cancer SGC-7901, hepatocarcinoma BEL-7402 and leukemia HL-60, were determined by a MTT and clone formation assay. The in vivo antitumor activity was evaluated by the antitumor bioassay against transplanted mouse solid tumor S180 and H22.

RESULTThe total-flavonoid inhibited cell proliferation of human tumor cell lines, and its activities are higher than that of vincristine. The total-flavonoid also showed a lower acute toxicity and the strong antitumor activity against transplanted mouse solid tumor S180 and H22 in vivo showing a positive correlation with the concentration. The inhibitory rates at the dose of 0.10 g x kg(-1) ip against S180 and H22 are 45.64% and 47.59%, respectively.

CONCLUSIONThe total-flavonoid from S. chamaejasme has antitumor activities in vivo and in vitro.

Animals ; Antineoplastic Agents, Phytogenic ; administration & dosage ; isolation & purification ; pharmacology ; Carcinoma, Hepatocellular ; pathology ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Dose-Response Relationship, Drug ; Female ; Flavonoids ; administration & dosage ; isolation & purification ; pharmacology ; HL-60 Cells ; Humans ; Liver Neoplasms ; pathology ; Liver Neoplasms, Experimental ; pathology ; Male ; Mice ; Mice, Inbred BALB C ; Neoplasm Transplantation ; Plants, Medicinal ; chemistry ; Sarcoma 180 ; pathology ; Stomach Neoplasms ; pathology ; Thymelaeaceae ; chemistry

PURPOSE: Traditionally, titanium miniplate has been used for rigid fixation of mandible fractures. However, the limitations of metal plate have been reported such as hypersensitivity, interference with the cranio-facial growth of growing child, secondary bone resorption around the plate, foreign body reaction, declination of primary callus formation, and bone atrophy, and so forth. Recently, biodegradable miniplate has been introduced and used as an alternative to the metal plate despite of its lower strength. This study evaluated the usefulness and stability of biodegradable plate and screw for treatment of mandible fractures. METHODS: In this study, 61 patients(92 areas) diagnosed as mandible fracture in the last 2 years have been reviewed. We used titanium plate and screw in 32 patients, and biodegradable plate and screw(INION?) in 29 patients. Stability of plates and screws, bony healing process and its side effects were observed by clinical and radiographic assessment. RESULTS: In the titanium material group, one of malocclusion, two of mouth opening limitation, three of pain, three of palpation were shown. The plate of six patients involved in these complications were removed. In the biodegradable group, two of mouth opening limitation, two of pain, one of localized wound infection were shown and one plate was removed secondarily. CONCLUSION: There was no statistical difference between two groups in bony healing and complication rates. Biodegradable implants show efficient stability during initial bone healing and low side effects in long-term follow up periods.
Absorbable Implants ; Atrophy ; Bone Resorption ; Bony Callus ; Child ; Follow-Up Studies ; Foreign-Body Reaction ; Fracture Fixation ; Humans ; Hypersensitivity ; Malocclusion ; Mandible ; Mouth ; Palpation ; Titanium ; Wound Infection

Adult ; Asymptomatic Diseases ; COVID-19/virology* ; COVID-19 Nucleic Acid Testing ; Disease Progression ; Female ; Humans ; Male ; RNA, Viral/isolation & purification* ; Reverse Transcriptase Polymerase Chain Reaction ; SARS-CoV-2/isolation & purification* ; Time Factors