Objective To investigate the clinical value of individualization of transrectal ultrasound guided prostate biopsy strategies for prostate cancer detection.Methods Ninety-seven patients who were considered having prostate cancer underwent individualization prostate biopsy strategies as individualization group.Fifty-two patients who were considered having prostate cancer underwent systemic 6-core prostate biopsy as control group.The detection rate of prostate cancer and rate of complications were compared between two groups.Results The detection rate of prostate cancer in individualization group was 43.3 ％(42/97),there was no significant difference in age and prostate volume between the prostate cancer patients and the non-prostate cancer patients(P＞0.05),but the prostate specific antigen(PSA)level of the prostate cancer patients was significantly higher than that of the non-prostate cancer patients[(26.4±2.9)μ g/L vs.(11.2±3.1)μ g/L,P=0.0000].The detection rate of prostate cancer in control group was 23.1％(12/52),the detection rate of prostate cancer in individualization group was significantly higher than that in control group(P=0.014).There was no significant difference in the rate of complications between two groups(P＞0.05).Conclusions The first detection rate of prostate cancer in individualization of transrectal ultrasound guided prostate biopsy strategies is higher than that by systemic 6-core prostate biopsy,and the rate of complications is not increased.The individualization of transrectal ultrasound guided prostate biopsy strategies for prostate cancer is good,safety,deserve clinical expansion.

Objective To investigate the relationship between genetic polymorphism in glutathione S-transferases T1, M1 and P1 and susceptibility to reflux esophagitis. Methods Genomic DNA was isolated from peripheral blood from the patients with reflux esophagitis (n=109), cases with negative endoscopy reflux disease (NERD) (n=97) and controls (n=97). Genes GSTM1 and GSTT1 were detected by multiplex PCR. A A→G polymorphism of codon 104 of the gene GSTP1 was detected using PCR-based restriction fragment length polymorphisms ( PCR-RFLP ) with Alw26I. Results The frequency of variant GSTP1 genotypes (*A/*B and*B/*B) was found to be 40.4% among the patients with reflux esophagitis compared with 24.7% among cases with gastroesophageal reflux without esophagitis and 21.6% among controls (P

[Objective]To determine the effect of Oxfenmino hydrochloric acid on delayed allergic reaction in mouse. [ Method ] 120 ICR mice weighing 18~22g, half female and half male, were randomly and averagely divided into 6 groups according to weight:control group and model group (treated with distilled water at a dosage of 10ml/kg), three Oxfenmino hydrochloric acid groups (treated with Oxfenmino hydrochloric acid at a dosage of 10.0mg/kg, 5.0mg/kg and 2.5mg/kg respectively) and positive drug group (treated with CsA at a dosage of 15mg/kg); they were orally administered one time every day. After a continuous week of administration, all the groups were smeared evenly with 1%DNFB solution on the abdomen for allergy except the control group. Five days after allergy, 1%DNFB solution was smeared to right ear of all mice to stimulate the allergic reaction except that the control group was smeared with non-allergenic substance. 24 hours after attack, the auricle swelling, spleen index and thymus index in right mouse were determined. [ Result] Different dosages of Oxfenmino hydrochloric acid benefited to reduce auricle swelling, spleen index and thymus index in mouse with allergic reaction induced by DNFB at different degree. [ Conclusion] Oxfenmino hydrochloric acid can inhibit the delayed allergic reaction in mouse induced by DNFB and depress the ability of specific cell-mediated immunity.

The authors retrospectively analysed 67(63 in cervical cord)cases of traumatic SCIWOAR with positive MR abnormalities. It was deduced that the cases of SCIWOAR might be related with (1)those traumatic forces resulting in SCI being not so heavy and severe, (2)the cases orignally with cervical spondylopathy,(3)the limitation of the conventional X-ray examination for SCI,and (4)the cases of children relativly rare in our country and hospital.The ALL hematomas are the pathognomonic signs in the case of the acute cervical SCI within one week.The analysis demonstrated that MR investigation of the traumatic spine could give the clinical activities the correct diagnosis of different degree of SCI and dependable reference.

A novel electrochemiluminescence ( ECL) method for the determination of L-cysteine ( L-Cys) was established. water-soluble CdS quantum dots ( QDs) with Cd2+rich surface were synthesized via a controllable one-poe approach. The mercapto group in L-cysteine molecule can specifically interact with excessive Cd2+on the surface of CdS QDs, resulting in enhancement of ECL intensity of the CdS QDs, which can be used for the detection of L-Cys. Under the optimal experimental conditions, the enhancement of ECL intensity was linear with the concentration of L-Cys in the range of 5. 0×10-9-1. 0×10-5 mol/L. The limit detection of (S/N=3) was 1. 2×10-9 mol/L. In comparison with other methods for detecting L-Cys, this method is more simple and selective, and can be applied to detect L-Cys in real sample with satisfactory results.

Objective To establish a rapid specific quantitative assay for Bacillus anthracis detection. Methods According to the principle of complex probe quantitative assay, the primers and quantitative probes targeted at chromatosome DNA rpoB were designed and applied to detect Bacillus anthracis. The influence factor of quantitative PCR were determined. Results The optimal system of this method was aquired: the length of quenching probe is 15mer,the ratio of fluorescent probe to quenching probe is 1/2 and the concentrtion of Mg 2+ is 3 mmol/L.The sensitivity of this assay for Bacillus anthracis is 10 3 copies. It can distinguish Bacillus anthracis from other closely related Bacillus. Conclusion The method can rapidly quantitatively detect the Bacillus anthracis with high sensitivity and specificity, it can be applied to clinical diagnosis.

Objective To investigate the effects of alprostadil on expression of proinflammatory cytokines in patients with severe acute pancreatitis (SAP) and evaluate the clinical efficacy.Methods Seventy-three SAP patients were collected from January 2014 to May 2016,and then were randomly divided into control group (n=37) and experimental group (n=36).On the basis of routine treatment,the experimental group patient was given alprostadil at a dose of 15μg/d.The expression of C-reactive protein (CRP),white blood cell (WBC) count,amylase,alanine aminotransferase (ALT),aspartate aminotransferase (AST),creatinine,serum proinflammatory cytokine tumor necrosis factor alpha (TNF-α),interleukin-1 beta (IL-1[β),interleukin-6 (IL-6) were detected in serum on the 1st,3rd and 7th day.Results The biochemical indexes and expression ofproinflammatory cytokines were significantly increased in the two groups on the 1st day,and decreased gradually,with a significant difference between the time points (P＜0.05),but the between-group difference was not significant (P＞0.05).These indexes were decreased significantly with the passage of time and there were significant differences between the two groups at the 3rd and 7th day (P＜0.05).Conclusion Alprostadil can effectively reduce the severity of early inflammatory reaction in SAP patients,and has important significance for improving the prognosis.

Objective To investigate the value and clinical significance of MRI in diagnosis of subcortical trabecular injury in knee.Methods45 patients with obvious pain but no fracture diagnosed by X-ray after knee trauma were scanned with MRI to analyze whether having injuries in subcortical trabecular and knee joint accessory structures.ResultsAll of the 45 cases had normal radiographic results in X-ray examination, but subcortical trabecular injury was found by MRI. MRI demonstrated irregular low signal in the subcortical region on both T1WI and T2WI. The high signal in fat suppressed T22subcortical trabecular and knee joint accessory structures.

The analysis of 87 cases of MR imaging given impression of dysraphic myelodysplasias and their clinical materials found tethered cord deformity in 70 and non - "tethered" cord or "free" cord abnormalities in 17.The purpose of this study was to show that tethered cord was the most important clinical entity resulting in nervous disfunction of the spinal cord of the children among various dysraphic abnormalities and that MRI investigation was the best choice in order to obtain early and correct diagnosis and management of dysraphic myelodysplasias and tethered cord particularly.

Objective To analyze the feature of joint osteochondral lesions under the magnetic resonance imaging (MRI). Methods The finding under MRI in the 68 focal osteochondral lesions were classified with the International Cartilage Repair Society (ICRS) classification, with the emphasis on articular cartilage and subchondral bone lesion.Results 58 lesions were classified as 4 grades: 2 focus of grade Ⅰ, 5 of grade Ⅱ, 14 of grade Ⅲ, 37 of grade Ⅳ. Other 10 focuses were grade Ⅰ of osteochondritis dissecans. MRI can discover subchondral bone lesion which couldn't be observed with arthroscopy. Conclusion MRI can accurately show the focal osteochondral lesions.