ObjectiveTo explore the influence of domestic violence on drinking and aggressive behaviors of adolescent， and to analyze the mediating role of psychological needs and moral disengagement. MethodsA cluster stratified random sampling method was adopted to select 1 280 students from 2 junior high schools and 2 high schools in Dazhou City， and all the enrolled students were assessed using Childhood Trauma Questionnaire-Short Form （CTQ-SF）， Basic Psychological Needs Scale （BPNS）， Moral Disengagement Scale （MDS） and Adolescent Health related Risky Behavior Inventory （AHRBI）. Then the relationship of domestic violence with drinking and aggressive behaviors， along with the mediating roles of psychological needs and moral disengagement were discussed through the chain mediating effect analysis. ResultsCTQ-SF score was negatively correlated with BPNS score （r=-0.160， P=0.012）， and positively correlated with MDS and AHRBI scores （r=0.330， 0.250， P<0.01）. BPNS score was negatively correlated with MDS and AHRBI scores （r=-0.220， -0.270， P<0.01）. MDS score was positively correlated with AHRBI score （r=0.420， P<0.01）. The direct mediation value of domestic violence to drinking and aggressive behaviors was 0.041 （P>0.05）， the mediation effect values of domestic violence to psychological needs， domestic violence to moral disengagement， psychological needs to moral disengagement， psychological needs to drinking and aggressive behaviors， and moral disengagement to drinking and aggression behaviors were -0.468， 0.536， -0.241， -0.412 and 0.094， respectively （P<0.05）. The total mediation effect value of domestic violence to psychological needs to moral disengagement to drinking and aggressive behaviors was 0.295 （P<0.05）. ConclusionPsychological needs and moral disengagement mediate the influence of domestic violence on adolescent drinking and aggressive behaviors.

OBJECTIVETo study the effect of different concentration of phytohemagglutinin (PHA) on mouse embryo development.

METHODIn experiment 1, crude and purified PHA extracted from Yunnan white kidney bean with different concentration were added into M16 culture medium, the final concentration of PHA were: 50, 100, 200, 500, 1 000, 2 000 and 5 000 mg x L(-1) respectively. 2-cell stage embryos were collected and cultured in PHA containing or control medium for 72-96 h and their development were recorded. In experiment 2, different stage of embryos from 1-cell to blastocyst were treated by different concentrations of PHA same as experiment 1 and 10 000 mg x L(-1) in culture medium for 24 h before washing and cultured in M16 + PVA without PHA to blastocyst or hatching blastocyst stage.

RESULTLow concentrations PHA at 50-100 mg x L(-1) promoted embryo development and increased the number of blastocyst stage embryos. In contrast, high concentrations of PHA (> 1 000 mg x L(-1)) blocked the embryos development from 1-cell to blastocyst stage and showed apoptosis morphology or death.

CONCLUSIONDepending on the concentrations, PHA from white kidney bean shown promotion or inhibition on mouse embryo development. 1-cell stage embryo shown more sensitive to PHA treatment than that of later stage embryos. Pretreatment 24 h in PHA containing medium can influence the further development of embryos. Low concentrations of PHA is benefit to embryo development, but high concentrations of PHA (> 1 000 mg x L(-1)) will block of the development of embryos.

Animals ; Embryo, Mammalian ; drug effects ; Embryonic Development ; drug effects ; Female ; Male ; Mice ; Phaseolus ; chemistry ; Phytohemagglutinins ; pharmacology ; Pregnancy

OBJECTIVETo discuss the feasibility of terminal intestinal exteriorization (exteriorization without ileostomy) in laparoscopic anterior resection for rectal cancer.

METHODSClinicopathological data of 77 patients undergoing laparoscopic anterior resection for low rectal cancer in our department from January 2011 to December 2013 were retrospectively analyzed. After laparoscopic rectal resection, 32 patients received terminal intestinal exteriorization (exteriorization group) and 45 patients received preventive ileostomy (ileostomy group). Anastomosis-related, stoma-related and intestinal stoma closure-related morbidity was compared between the two groups.

RESULTSThere were no significant differences in operative time, blood loss and overall hospital stay between the two groups (all P>0.05). The total hospital cost was (5.39±1.74)×10(4) yuan in the exteriorization group, and (6.98±1.37)×10(4) yuan in the ileostomy group(P<0.01). The incidences of postoperative anastomotic fistula was not significantly different between the two groups(P>0.05). Three patients(9.4%) developed anastomotic leak in the exteriorization group and 2(4.4%) in the ileostomy group. The anastomotic leak was managed by opening the external intestinal wall and maturating an ileostomy under local anaesthesia. All these 5 patients were cured with nutritional support, antibiotics, continuous local drainage. In the exteriorization group, 5 patients had complications related to stoma and intestinal stoma closure operation(15.6%), which was lower than(42.2%) in the ileostomy group(P=0.013).

CONCLUSIONTerminal intestinal exteriorization in laparoscopic anterior resection is a safe and feasible surgical procedure with little trauma and less hospital cost, which can be an alternative as a prophylactic treatment for patients with high risk of anastomotic leak.

Anastomosis, Surgical ; Anastomotic Leak ; Drainage ; Humans ; Ileostomy ; Laparoscopy ; Length of Stay ; Postoperative Complications ; Rectal Neoplasms ; Retrospective Studies ; Surgical Stomas