Objective To improve the awareness of the treatment and mechanisms of the gastric mucosa-associated lymphoid tissue lymphoma with secondary thrombocytosis. Methods One case of gastric MALT lymphoma with secondary thrombocytosis was reported and reviewed the literatures. Results The patient received anti-Hp therapy,with remission of gastric MALT lymphoma, platelet count also will return to normal levels. Conclusion It is shown that anti-Hp therapy of gastric MALT lymphoma with secondary thrombocytosis is an effective treatment.

Objective To investigate the effects and potential mechanism of irinotecan (CPT-11), an antitumor drug, on human colorectal cancer cell line HT-29 and its impact on 4-amion pyridine (4-AP), a kalium ion channel blocker. Methods The effects of CPT-11, 4-AP and combination of two drugs on proliferation and invasion of HT-29 cells were measured by MTT and Transwell assay respectively. The impact of CPT-11 or 4-AP on cell apoptosis was determined by flow cytometry with Annexin-V and PI staining. The current of ATP sensitive potassium ion (IKATP) was measured by patch clamp. Results The CPT-11 could inhibit proliferation of HT-29 cells at dose from 1.0 to 64.0 μg/ml in dose-and time-dependent manners. Whereas the above effect was enhanced when CPT-11 combined with 4-AP (1.0 mmol/L). The administration of CPT-11 (16.0 μg/ml) or 4-AP (1.0 mmol/L) significantly induced the cell apoptosis and inhibited the invasion of HT-29 cells, furthermore, these effects could be enhanced by combination of two drugs. And the different concentrations of CPT-11 reduced the IKATP of cell membrane in negative dose-dependent manner. Conclusions The effects of CPT-11 on HT-29 cells, such as reducing proliferation and invasion as well as inducing the apoptosis, can be enhanced by 4-AP, which may be related to inhibition of ATP-sensitive potassium channels.

Objective To study the effects of Irinotecan (CPT-11) on human colorectal cancer HCT-116 and HT-29 cells and investigate the potential mechanisms.Methods The effect of Irinotecan on the proliferation of HCT-116 and HT-29 cells was determined by MTT assays.The invasive capacity was measured by transwell assays,and the apoptosis of the tumor cells was detected by flow cytometry after stained with Annexin-V and PI.The difference between the current of ATP-sensitive potassium ion of HCT-116 and HT-29 was examined by patch clamp.Results It was found that 1.0-64.0 μg/ml CPT-11 could inhibit the proliferation and the invasive capacity of HCT-116 and HT-29 cells at both dose-and time-dependent manner.The IC_(50) of HCT-116 and HT-29 were 39.3 and 19.5 μg/ml respectively.Cytometry showed that the apoptotic rates were increased from 14.8% and 9.3% to 36.9% and 27.9% after the treatment of 32.0 μg/ml and 16.0 μg/ ml CPT-11,which were close to their IC_(50).The proportion of G_0/G_1 and S of HCT-116 and HT-29 was enhanced from 27.4% and 17.4% to 95.9% and 98.2%.Transwell assay indicated that the invasiveness of HCT-116 and HT-29 was reduced by 40.8% and 47.5%.The patch clamp showed that CPT-11 reduced the I_(KATP) of cell membrane at a negative dose-dependent manner.Conclusion CPT-11 could have a significant impact on the proliferation,invasiveness,cell cycle,and the apoptosis of human colorectal cancer cell HCT-116 and HT-29.HT-29 was more sensitive to CPT-11 than HCT-116.The inhibitory effect of CPT-11 on cell proliferation might be linked to its inhibition of ATPsensitive potassium channel.

Objective To compare the preoperative eternal nutrition bowel preparation method with the conventional method in the intestinal cleansing effect,postoperative infection and postoperative complications of gastrointestinal function recovery time,and to provide reference for selection for our clinical preoperative practice.Methods Computer Retrieval of Chinese Biomedical Literature Database,VIP Chinese Science and Technology Journal Full-text Database and China Academic Journal was conducted.The time limit was from establishment of database till May 2013.The research type was randomized controlled trial.Two investigators independently screened,assessed quality and extracted data according to the inclusion and exclusion criteria for literatures,and cross-checked them using RevMan 5.0 software for Metaanalysis,then applied the grading method recommended by the GRADE grading system.Results Ultimately seven RCTs were included,with a total of 478 cases of colorectal cancer patients.Compared with conventional preoperative bowel preparation,eternal nutrition method showed similar bowel cleansing effects and the incidence of complications,but it could shorten the postoperative recovery of gastrointestinal function time,lower positive rate of abdomen,intestine shedding of tumor cells,improve postoperative albumin and transferritin levels.Conclusions Eternal nutrition alternative method for bowel preparation before colorectal surgery is safe and effective.

Objective To sdudy the expression of p53 and nm23-H1 proteins and their clinical significance in nasopharyngeal carcinoma.Methods The expression of p53 and nm23-H1 proteins were detected by immunohistochemistry method in 40 cases with nasopharyngeal carcinoma and 22 cases with chronic inflammation of nasopharyngeal mucosa tissues.Results Positive rates of p53 and nm23-H1 in chronic nasopharyngitis group were 1.0 ％,27.2 ％,and in the NPC group were 92.5 ％,55.0 ％.There were 9 cases with the positive expression of p53 in 22 cases of nasopharyngeal carcinoma tissues with the positive expression of nm23-H1 (40.9 ％).There were 17 cases with the positive expression of p53 in 18 cases with the negative expression of nm23-H1 (94.4 ％).The expression of p53 and nm23-H1 proteins in nasopharyngeal carcinoma were much higher than that in chronic inflammation of nasopharyngeal mucosa tissues.The expression of p53 protein in nasopharyngeal carcinoma with metastatic lymph node was higher than that in nasopharyngeal carcinoma without metastatic lymph node,but nm23-H1 protein lower.The expression of p53 protein was positively correlated with the metastasis,clinnical staging and pathological classification but not correlated with T classification of nasopharyngeal carcinoma.The expression of nm23-H1 protein was negative correlation with the metastasis and clinical staging of nasopharyngeal carcinoma.Conclusion p53 and nm23-H1 play important coordinated regulation roles in the carcinogenesis,development and metastasis of nasopharyngeal carcinoma and will probably become the key biological marks in the judging and evaluating prognosis of nasopharyngeal carcinoma.

Objective To evaluate the effect of preoperative ambroxol aerosol inhalation on postoperative lung complications in patients with chest and abdominal surgery.Methods The randomized controlled trials (RCTs) taking preoperative ambroxol aerosol inhalation interventions were collected from the databases of Chinese Technology Journal Database,China National Knowledge Internet and Wanfang Database.Data were analyzed with RevMan 5.0 software.Results Eleven articles with 1 160 patients met the inclusion criteria.There was a certain heterogeneity across the analysis,which might result from kind of included patients,inhalation method,difference of the medication,inhalation time,etc,according to subgroup analysis.The result showed that compared with the control group,patients in the experimental group had lower incidence of postoperative lung complications and the differences were not statistically significant.Conclusions The Meta analysis had identified a significant effect of preoperative ambroxol aerosol inhalation on postoperative lung complications in patients with chest and abdominal surgery,but high quality were in need.

Qian Hu is the roots of Peucedanum praeruptorum Dunn. The object of the present paper was to determine the effects of water extract (QH-I) and petroleum ether extract (QH-M) of Qian Hu on smooth muscle contractility of isolated rabbit pulmonary artery rings. QH-I and QH-M antagonized pulmonary artery rings conlraction elicited by NE as well as KCl.QH-I and QH-M caused relaxation of rabbit pulmonary artery rings precontracted with NE as well as KCl. The contraction dose-response curves of pulmonary artery rings with NE were shifted to the right and the maximal contraction response were inhibited 73.1 ?8.0% and 43 .6? 4.2% by 1 .6 ? 10-5% and 8.0 ? 10-5%, QH-M, pD'2 = 4.72 ?0.43. These results indicate that Qian Hu may relax pulmonary artery smooth muscle directly as a Ca2+ antagonist

Objective To investigate the dynamic changes of ATP content in rat cerebral cortex after transient ischemia followed by reperfusion and the relationship between the change of energy and the recovery of neural function.Methods The rats were subjected to 10 min of middle cerebral artery occlusion (MCAO). At the time point of 0 h, 1 h, 3 h, 6 h, 12 h, 24 h and 72 h after reperfusion, ATP contents of frontal and parietal cortex were measured by capillary zone electrophoresis.Results At the end of 10 min ischemia, ATP content fell dramatically to less than 20% of the control level. After reperfusion, ATP content recovered gradually. After 1 h, 3 h, 6 h and 12 h of reperfusion, ATP content returned to 70.5%, 65.7%, 84.8% and 86.9% of the control level ( P=0.052, 0.030, 0.332 and 0.491). From 24 h on until 72 h after reperfusion, ATP content decreased again, reaching half of the control level ( P=0.003 and P=0.023). After 10 min ischemia, limb function recovered gradually and completely at last. From 24 h on until 72 h after reperfusion, unwillingness of action and eating was found.Conclusions The recovery of cellular energy system function is delayed even though the reperfusion is in time after transient cerebral ischemia. Furthermore, secondary failure of cellular energy system function occurrs with the reperfusion proceeding. These phenomena are probably responsible for the delayed recovery of neural function after cerebral ischemia in spite of reperfusion.

Objective To develop a method for the determination of trace mercury in waste water by atomic fluorescence spectrometry. Methods The mercury vapor was generated with the hydride generation agent potassium tetrahydroborate (KBH4) in hydrochloric acid. The optimum working conditions of the instrument were studied, including flow rate of carrier gas, concentration of potassium tetrahydroborate solution and acidity of sample solution. Results Under the optimum condition, the linear range of determination was 0-20 ng/ml, the detection limit was 0.04 ng/ml, the relative standard deviation was 1.9% (n=11) for 5 ng/ml Hg, the recovery rate was in the range of 96%-104%. Conclusion This method is applicable to the determination of mercury in the waste water.

Objective To develop a new method for the determination of trace silver in water by flame atomic absorption(FAAS) after cloud point extraction.Methods Diethyldithiophosphate(DDTP) and triton X-114 were respectively used as the chelating agent and surfactant.The effects of experimental conditions such as acidity,concentration of chelating agent,surfactant and methanol,equilibration temperature and time,interference ion on cloud point extraction were investigated.Results Under the optimum condition,0.5 ml of 0.1 mol/L DDTP solution,0.5 ml of 50 g/L Triton X-114 and 5 ml of 1 mol/L hydrochloric acid were added and the volume was made up to 50 ml.The mixture was heated in a thermostatic bath at 40 ℃ for 15 min,the linear range of determination for silver was 0-100 ng/ml,the equation of linear regression was A = 0.005 8cAg+ 0.001 6,r = 0.999.The detection limit was 0.83 ng/ml,the recovery rate was in the range of 97%-105%,and the relative standard deviation was 3.1%(n=11) for 20 ng/ml Ag.There was no significant interference for silver solution containing 5 000 times of K+,Na+,Ca2,Mg2,NO3,SO4 + +-2-and Cl-,1 000 times of Al3+ and Zn2+,500 times of Fe2+ and Mn2+,100 times of Pb2+ and Cu2+.Conclusion The method is rapid,accurate,simple and applicable to the determination of trace silver in water samples.