Objective:To improve the comprehensive quality of medical students through developing the second class teaching of histology and embryology in the college of traditional Chinese medicine.Methods: Combined with the Problem-based learning guiding method, using a variety of teaching methods of teaching windows, self atlas, open labs and knowledge competition to carry vivid histology and embryology second class.Results: The second class may improve the students' interest in learning, train the practical ability and promote teamwork.Conclusion: The second class is a strong complement to the traditional form of teaching histology and embryology, effectiveness of teaching innovation is good in practice and contributes to the cultivation of talents of traditional Chinese medicine.