Purpose: This study was conducted to deeply explore the work experiences of nurses in COVID-19 isolation wards. Methods: Data were collected through one-on-one in-depth interviews with 11 nurses working in COVID-19 isolation wards from November 3 to November 23, 2023. The data were analyzed using Braun and Clarke's inductive thematic analysis. Results: The study identified three categories, seven themes, and 16 sub-themes. The categories were: ‘the reality of unprepared COVID-19 isolation wards’, ‘the ongoing process of change and coordination’, and ‘experiencing confusion in the process of returning to normal’. A common experience among nurses was ‘confusion due to changes’. Initially, there was confusion caused by the unfamiliar environment and lack of a work manual. Subsequently, confusion arose due to changes in the direction of the COVID-19 response. Finally, they experienced confusion in the process of returning to normal life, continually facing uncertainty. Conclusion Strategies are needed to improve the work environment of nurses in isolation wards for future infectious diseases and pandemics. Allocating nursing staff considering infection control tasks and patient care demands, and addressing communication problems in isolation wards is necessary. Routine education on infection control and preparation for emerging infectious diseases should be implemented, and an operational manual for duties in isolation ward should be prepared. Additionally, promoting psychological support systems for disaster response personnel and developing policies to alleviate physical difficulties are needed.

Purpose: This study was conducted to deeply explore the work experiences of nurses in COVID-19 isolation wards. Methods: Data were collected through one-on-one in-depth interviews with 11 nurses working in COVID-19 isolation wards from November 3 to November 23, 2023. The data were analyzed using Braun and Clarke's inductive thematic analysis. Results: The study identified three categories, seven themes, and 16 sub-themes. The categories were: ‘the reality of unprepared COVID-19 isolation wards’, ‘the ongoing process of change and coordination’, and ‘experiencing confusion in the process of returning to normal’. A common experience among nurses was ‘confusion due to changes’. Initially, there was confusion caused by the unfamiliar environment and lack of a work manual. Subsequently, confusion arose due to changes in the direction of the COVID-19 response. Finally, they experienced confusion in the process of returning to normal life, continually facing uncertainty. Conclusion Strategies are needed to improve the work environment of nurses in isolation wards for future infectious diseases and pandemics. Allocating nursing staff considering infection control tasks and patient care demands, and addressing communication problems in isolation wards is necessary. Routine education on infection control and preparation for emerging infectious diseases should be implemented, and an operational manual for duties in isolation ward should be prepared. Additionally, promoting psychological support systems for disaster response personnel and developing policies to alleviate physical difficulties are needed.

Purpose: This study was conducted to deeply explore the work experiences of nurses in COVID-19 isolation wards. Methods: Data were collected through one-on-one in-depth interviews with 11 nurses working in COVID-19 isolation wards from November 3 to November 23, 2023. The data were analyzed using Braun and Clarke's inductive thematic analysis. Results: The study identified three categories, seven themes, and 16 sub-themes. The categories were: ‘the reality of unprepared COVID-19 isolation wards’, ‘the ongoing process of change and coordination’, and ‘experiencing confusion in the process of returning to normal’. A common experience among nurses was ‘confusion due to changes’. Initially, there was confusion caused by the unfamiliar environment and lack of a work manual. Subsequently, confusion arose due to changes in the direction of the COVID-19 response. Finally, they experienced confusion in the process of returning to normal life, continually facing uncertainty. Conclusion Strategies are needed to improve the work environment of nurses in isolation wards for future infectious diseases and pandemics. Allocating nursing staff considering infection control tasks and patient care demands, and addressing communication problems in isolation wards is necessary. Routine education on infection control and preparation for emerging infectious diseases should be implemented, and an operational manual for duties in isolation ward should be prepared. Additionally, promoting psychological support systems for disaster response personnel and developing policies to alleviate physical difficulties are needed.

Purpose: This study was conducted to deeply explore the work experiences of nurses in COVID-19 isolation wards. Methods: Data were collected through one-on-one in-depth interviews with 11 nurses working in COVID-19 isolation wards from November 3 to November 23, 2023. The data were analyzed using Braun and Clarke's inductive thematic analysis. Results: The study identified three categories, seven themes, and 16 sub-themes. The categories were: ‘the reality of unprepared COVID-19 isolation wards’, ‘the ongoing process of change and coordination’, and ‘experiencing confusion in the process of returning to normal’. A common experience among nurses was ‘confusion due to changes’. Initially, there was confusion caused by the unfamiliar environment and lack of a work manual. Subsequently, confusion arose due to changes in the direction of the COVID-19 response. Finally, they experienced confusion in the process of returning to normal life, continually facing uncertainty. Conclusion Strategies are needed to improve the work environment of nurses in isolation wards for future infectious diseases and pandemics. Allocating nursing staff considering infection control tasks and patient care demands, and addressing communication problems in isolation wards is necessary. Routine education on infection control and preparation for emerging infectious diseases should be implemented, and an operational manual for duties in isolation ward should be prepared. Additionally, promoting psychological support systems for disaster response personnel and developing policies to alleviate physical difficulties are needed.

OBJECTIVES: Sodium hypochlorite (NaOCl) is an excellent bactericidal agent, but it is detrimental to stem cell survival, whereas intracanal medicaments such as calcium hydroxide (Ca[OH]2) promote the survival and proliferation of stem cells. This study evaluated the effect of sequential NaOCl and Ca[OH]2 application on the attachment and differentiation of dental pulp stem cells (DPSCs). MATERIALS AND METHODS: DPSCs were obtained from human third molars. All dentin specimens were treated with 5.25% NaOCl for 30 min. DPSCs were seeded on the dentin specimens and processed with additional 1 mg/mL Ca[OH]2, 17% ethylenediaminetetraacetic acid (EDTA) treatment, file instrumentation, or a combination of these methods. After 7 day of culture, we examined DPSC morphology using scanning electron microscopy and determined the cell survival rate with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. We measured cell adhesion gene expression levels after 4 day of culture and odontogenic differentiation gene expression levels after 4 wk using quantitative real-time polymerase chain reaction. RESULTS: DPSCs did not attach to the dentin in the NaOCl-treated group. The gene expression levels of fibronectin-1 and secreted phosphoprotein-1 gene in both the Ca[OH]2- and the EDTA-treated groups were significantly higher than those in the other groups. All Ca[OH]2-treated groups showed higher expression levels of dentin matrix protein-1 than that of the control. The dentin sialophosphoprotein level was significantly higher in the groups treated with both Ca[OH]2 and EDTA. CONCLUSIONS: The application of Ca[OH]2 and additional treatment such as EDTA or instrumentation promoted the attachment and differentiation of DPSCs after NaOCl treatment.
Calcium Hydroxide ; Cell Adhesion ; Cell Differentiation ; Cell Survival ; Dental Pulp* ; Dentin* ; Edetic Acid ; Gene Expression ; Humans* ; Microscopy, Electron, Scanning ; Molar, Third ; Real-Time Polymerase Chain Reaction ; Sodium Hypochlorite ; Stem Cells*

It has been shown that dysregulation of IGF-1 signaling is associated with tumor incidence and progression, whereas blockade of the signaling can effectively inhibit carcinogenesis. Although several mechanisms of anticancer activity of quercetin were proposed, molecular targets of quercetin have not been identified yet. Hence, we assessed the effect of quercetin on IGF-1 signaling inhibition in BK5.IGF-1 transgenic (Tg) mice, which over-expresses IGF-1 in the skin epidermis. A quercetin diet (0.02% wt/wt) for 20 weeks remarkably delayed the incidence of skin tumor by 2 weeks and reduced tumor multiplicity by 35% in a 7,12-dimethylbenz(a)anthracene (DMBA)-tetradecanoyl phorbol-13-acetate (TPA) two stage mouse skin carcinogenesis protocol. Moreover, skin hyperplasia in Tg mice was significantly inhibited by a quercetin supplementation. Further analysis of the MT1/2 skin papilloma cell line showed that a quercetin treatment dose dependently suppressed IGF-1 induced phosphorylation of the IGF-1 receptor (IGF-1R), insulin receptor substrate (IRS)-1, Akt and S6K; however, had no effect on the phosphorylation of PTEN. Additionally, the quercetin treatment inhibited IGF-1 stimulated cell proliferation in a dose dependent manner. Taken together, these data suggest that quercetin has a potent anticancer activity through the inhibition of IGF-1 signaling.
Animals ; Anticarcinogenic Agents* ; Cell Line ; Cell Proliferation ; Diet ; Epidermis ; Hyperplasia ; Incidence ; Insulin-Like Growth Factor I ; Mice* ; Papilloma ; Phosphorylation ; Quercetin* ; Receptor, IGF Type 1 ; Receptor, Insulin ; Skin Neoplasms* ; Skin*

Background: Unemployment and the consequential mental health issues are emerging as a major social concern. This study aimed to evaluate factors associated with depression and suicidality among unemployed individuals in the Jeju area. Methods: Data from 624 unemployed adults who participated in a community survey were analyzed. Self-report questionnaires were completed by all participants; demographic variables and the Patient Health Questionnaire-9 were used to assess depression. The Mini International Neuropsychiatric Interview suicidality module was used to obtain information regarding suicidal ideation, suicide plans, and suicide attempts. Results: The prevalence of depressive symptoms among the unemployed population in Jeju area was 28.4%. Factors associated with suicidality included age, gender, unemployment period, job application period, and depression. Furthermore, unemployed participants who had an opportunity to receive vocational education had fewer symptoms of depression regardless of the number of vocational education sessions. A multivariate regression model demonstrated that depression (β = 0.51, p<0.001) was significantly correlated with suicidality. Conclusion The findings advance our understanding of the relationship between unemployment and psychological factors such as depression symptoms and suicidality. Further studies are required to determine the underlying mechanisms to aid in the development of preventive and treatment strategies.

A 10-year-old spayed female Maltese presented with purpura and hematemesis. Initial laboratory evaluation revealed immune-mediated thrombocytopenia, but evidence of hemolytic anemia was not identified. Three milligrams of human intravenous immunoglobulin (hIVIG) was administered for 3 hours following prednisolone and mycophenolate mofetil. A pale mucous membrane was identified, and the packed cell volume decreased by 3%. Blood film examination revealed significant spherocytosis with auto-agglutination. Blood transfusions and immunosuppression were continued for 4 days, and hIVIG was discontinued. This report describes a case of increased immune-mediated hemolysis after hIVIG administration, possibly due to new-onset immune-mediated hemolytic anemia or enhanced immunogenicity.

Peritoneal lymphomatosis (PL) is a rare lymphoid neoplasm in dogs. A nine-year-old spayed female Labrador retriever presented with pleural and peritoneal effusions. Diagnostic imaging revealed diffuse nodular to massive lesions in the mesentery, particularly in the caudal abdomen. While the superficial lymph nodes did not show significant changes, enlargement was observed in the intra-abdominal and intra-thoracic lymph nodes. Cytological and flow cytometric analyses of the effusion indicated the presence of large B-cell lymphocytes expressing CD3-/CD5-/CD14-/CD21-/CD34+/CD45+/CD79a+. PL was diagnosed using diagnostic imaging and fluid analysis. This case report highlights the clinical and diagnostic features of canine PL.

BACKGROUND: Omega-3 fatty acids derived from fish oil have been reported to exert a beneficial effect on reducing cardiovascular disease. Reports about their mechanism have generated several interesting findings, including a change in small dense low density lipoprotein (sdLDL) cholesterol proportion, adiponectin, and apolipoprotein B (apoB), in addition to changes in the lipid profile. The principal objective of our study was to evaluate the effects of omega-3 fatty acids on plasma sdLDL, adiponectin, apoB100, and B48 in type 2 diabetic patients with hypertriglyceridemia. METHODS: We randomized 28 type 2 diabetic patients in a placebo-controlled, double-blind trial to receive either omega-3 fatty acids or placebo, both administered at a dose of 4 g daily for 12 weeks. LDL subfractions prior to and after treatment were separated via low-speed ultracentrifugation and analyzed via immunoelectrophoresis. Adiponectin, apoB100, and B48 levels were measured using an ELISA kit. RESULTS: sdLDL proportions were reduced in the omega-3 fatty acids group by 11% after 12 weeks of treatment (n = 17, P = 0.001), and were reduced by 4% in the control group (n = 11, P = 0.096). The patients receiving the omega-3 fatty acids evidenced a significant reduction in the levels of triglyceride (P = 0.001), apoB100, and B48 after 12 weeks (P = 0.038 and P = 0.009, respectively) relative to the baseline. Omega-3 fatty acids supplementation increased fasting blood glucose (P = 0.011), but the levels of HbA1c in each group did not change to a statistically significance degree. The adiponectin value was not reduced in the omega-3 fatty acids group (P = 0.133); by way of contrast, the placebo group evidenced a significant reduction in adiponectin value after 12 weeks (P = 0.002). CONCLUSION: Omega-3 fatty acid treatment proved effective in the reduction of atherogenic sdLDL and apoB in type 2 diabetic patients (Clinical trials reg. no. NCT 00758927, clinicaltrials.gov).
Adiponectin ; Apolipoprotein B-48 ; Apolipoproteins ; Apolipoproteins B ; Blood Glucose ; Cardiovascular Diseases ; Cholesterol ; Diabetes Mellitus, Type 2 ; Enzyme-Linked Immunosorbent Assay ; Fasting ; Fatty Acids, Omega-3 ; Humans ; Immunoelectrophoresis ; Lipoproteins ; Plasma ; Ultracentrifugation