Purpose: This study was conducted to deeply explore the work experiences of nurses in COVID-19 isolation wards. Methods: Data were collected through one-on-one in-depth interviews with 11 nurses working in COVID-19 isolation wards from November 3 to November 23, 2023. The data were analyzed using Braun and Clarke's inductive thematic analysis. Results: The study identified three categories, seven themes, and 16 sub-themes. The categories were: ‘the reality of unprepared COVID-19 isolation wards’, ‘the ongoing process of change and coordination’, and ‘experiencing confusion in the process of returning to normal’. A common experience among nurses was ‘confusion due to changes’. Initially, there was confusion caused by the unfamiliar environment and lack of a work manual. Subsequently, confusion arose due to changes in the direction of the COVID-19 response. Finally, they experienced confusion in the process of returning to normal life, continually facing uncertainty. Conclusion Strategies are needed to improve the work environment of nurses in isolation wards for future infectious diseases and pandemics. Allocating nursing staff considering infection control tasks and patient care demands, and addressing communication problems in isolation wards is necessary. Routine education on infection control and preparation for emerging infectious diseases should be implemented, and an operational manual for duties in isolation ward should be prepared. Additionally, promoting psychological support systems for disaster response personnel and developing policies to alleviate physical difficulties are needed.

Purpose: This study was conducted to deeply explore the work experiences of nurses in COVID-19 isolation wards. Methods: Data were collected through one-on-one in-depth interviews with 11 nurses working in COVID-19 isolation wards from November 3 to November 23, 2023. The data were analyzed using Braun and Clarke's inductive thematic analysis. Results: The study identified three categories, seven themes, and 16 sub-themes. The categories were: ‘the reality of unprepared COVID-19 isolation wards’, ‘the ongoing process of change and coordination’, and ‘experiencing confusion in the process of returning to normal’. A common experience among nurses was ‘confusion due to changes’. Initially, there was confusion caused by the unfamiliar environment and lack of a work manual. Subsequently, confusion arose due to changes in the direction of the COVID-19 response. Finally, they experienced confusion in the process of returning to normal life, continually facing uncertainty. Conclusion Strategies are needed to improve the work environment of nurses in isolation wards for future infectious diseases and pandemics. Allocating nursing staff considering infection control tasks and patient care demands, and addressing communication problems in isolation wards is necessary. Routine education on infection control and preparation for emerging infectious diseases should be implemented, and an operational manual for duties in isolation ward should be prepared. Additionally, promoting psychological support systems for disaster response personnel and developing policies to alleviate physical difficulties are needed.

Purpose: This study was conducted to deeply explore the work experiences of nurses in COVID-19 isolation wards. Methods: Data were collected through one-on-one in-depth interviews with 11 nurses working in COVID-19 isolation wards from November 3 to November 23, 2023. The data were analyzed using Braun and Clarke's inductive thematic analysis. Results: The study identified three categories, seven themes, and 16 sub-themes. The categories were: ‘the reality of unprepared COVID-19 isolation wards’, ‘the ongoing process of change and coordination’, and ‘experiencing confusion in the process of returning to normal’. A common experience among nurses was ‘confusion due to changes’. Initially, there was confusion caused by the unfamiliar environment and lack of a work manual. Subsequently, confusion arose due to changes in the direction of the COVID-19 response. Finally, they experienced confusion in the process of returning to normal life, continually facing uncertainty. Conclusion Strategies are needed to improve the work environment of nurses in isolation wards for future infectious diseases and pandemics. Allocating nursing staff considering infection control tasks and patient care demands, and addressing communication problems in isolation wards is necessary. Routine education on infection control and preparation for emerging infectious diseases should be implemented, and an operational manual for duties in isolation ward should be prepared. Additionally, promoting psychological support systems for disaster response personnel and developing policies to alleviate physical difficulties are needed.

Purpose: This study was conducted to deeply explore the work experiences of nurses in COVID-19 isolation wards. Methods: Data were collected through one-on-one in-depth interviews with 11 nurses working in COVID-19 isolation wards from November 3 to November 23, 2023. The data were analyzed using Braun and Clarke's inductive thematic analysis. Results: The study identified three categories, seven themes, and 16 sub-themes. The categories were: ‘the reality of unprepared COVID-19 isolation wards’, ‘the ongoing process of change and coordination’, and ‘experiencing confusion in the process of returning to normal’. A common experience among nurses was ‘confusion due to changes’. Initially, there was confusion caused by the unfamiliar environment and lack of a work manual. Subsequently, confusion arose due to changes in the direction of the COVID-19 response. Finally, they experienced confusion in the process of returning to normal life, continually facing uncertainty. Conclusion Strategies are needed to improve the work environment of nurses in isolation wards for future infectious diseases and pandemics. Allocating nursing staff considering infection control tasks and patient care demands, and addressing communication problems in isolation wards is necessary. Routine education on infection control and preparation for emerging infectious diseases should be implemented, and an operational manual for duties in isolation ward should be prepared. Additionally, promoting psychological support systems for disaster response personnel and developing policies to alleviate physical difficulties are needed.

OBJECTIVES: Sodium hypochlorite (NaOCl) is an excellent bactericidal agent, but it is detrimental to stem cell survival, whereas intracanal medicaments such as calcium hydroxide (Ca[OH]2) promote the survival and proliferation of stem cells. This study evaluated the effect of sequential NaOCl and Ca[OH]2 application on the attachment and differentiation of dental pulp stem cells (DPSCs). MATERIALS AND METHODS: DPSCs were obtained from human third molars. All dentin specimens were treated with 5.25% NaOCl for 30 min. DPSCs were seeded on the dentin specimens and processed with additional 1 mg/mL Ca[OH]2, 17% ethylenediaminetetraacetic acid (EDTA) treatment, file instrumentation, or a combination of these methods. After 7 day of culture, we examined DPSC morphology using scanning electron microscopy and determined the cell survival rate with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. We measured cell adhesion gene expression levels after 4 day of culture and odontogenic differentiation gene expression levels after 4 wk using quantitative real-time polymerase chain reaction. RESULTS: DPSCs did not attach to the dentin in the NaOCl-treated group. The gene expression levels of fibronectin-1 and secreted phosphoprotein-1 gene in both the Ca[OH]2- and the EDTA-treated groups were significantly higher than those in the other groups. All Ca[OH]2-treated groups showed higher expression levels of dentin matrix protein-1 than that of the control. The dentin sialophosphoprotein level was significantly higher in the groups treated with both Ca[OH]2 and EDTA. CONCLUSIONS: The application of Ca[OH]2 and additional treatment such as EDTA or instrumentation promoted the attachment and differentiation of DPSCs after NaOCl treatment.
Calcium Hydroxide ; Cell Adhesion ; Cell Differentiation ; Cell Survival ; Dental Pulp* ; Dentin* ; Edetic Acid ; Gene Expression ; Humans* ; Microscopy, Electron, Scanning ; Molar, Third ; Real-Time Polymerase Chain Reaction ; Sodium Hypochlorite ; Stem Cells*

It has been shown that dysregulation of IGF-1 signaling is associated with tumor incidence and progression, whereas blockade of the signaling can effectively inhibit carcinogenesis. Although several mechanisms of anticancer activity of quercetin were proposed, molecular targets of quercetin have not been identified yet. Hence, we assessed the effect of quercetin on IGF-1 signaling inhibition in BK5.IGF-1 transgenic (Tg) mice, which over-expresses IGF-1 in the skin epidermis. A quercetin diet (0.02% wt/wt) for 20 weeks remarkably delayed the incidence of skin tumor by 2 weeks and reduced tumor multiplicity by 35% in a 7,12-dimethylbenz(a)anthracene (DMBA)-tetradecanoyl phorbol-13-acetate (TPA) two stage mouse skin carcinogenesis protocol. Moreover, skin hyperplasia in Tg mice was significantly inhibited by a quercetin supplementation. Further analysis of the MT1/2 skin papilloma cell line showed that a quercetin treatment dose dependently suppressed IGF-1 induced phosphorylation of the IGF-1 receptor (IGF-1R), insulin receptor substrate (IRS)-1, Akt and S6K; however, had no effect on the phosphorylation of PTEN. Additionally, the quercetin treatment inhibited IGF-1 stimulated cell proliferation in a dose dependent manner. Taken together, these data suggest that quercetin has a potent anticancer activity through the inhibition of IGF-1 signaling.
Animals ; Anticarcinogenic Agents* ; Cell Line ; Cell Proliferation ; Diet ; Epidermis ; Hyperplasia ; Incidence ; Insulin-Like Growth Factor I ; Mice* ; Papilloma ; Phosphorylation ; Quercetin* ; Receptor, IGF Type 1 ; Receptor, Insulin ; Skin Neoplasms* ; Skin*

Background: Unemployment and the consequential mental health issues are emerging as a major social concern. This study aimed to evaluate factors associated with depression and suicidality among unemployed individuals in the Jeju area. Methods: Data from 624 unemployed adults who participated in a community survey were analyzed. Self-report questionnaires were completed by all participants; demographic variables and the Patient Health Questionnaire-9 were used to assess depression. The Mini International Neuropsychiatric Interview suicidality module was used to obtain information regarding suicidal ideation, suicide plans, and suicide attempts. Results: The prevalence of depressive symptoms among the unemployed population in Jeju area was 28.4%. Factors associated with suicidality included age, gender, unemployment period, job application period, and depression. Furthermore, unemployed participants who had an opportunity to receive vocational education had fewer symptoms of depression regardless of the number of vocational education sessions. A multivariate regression model demonstrated that depression (β = 0.51, p<0.001) was significantly correlated with suicidality. Conclusion The findings advance our understanding of the relationship between unemployment and psychological factors such as depression symptoms and suicidality. Further studies are required to determine the underlying mechanisms to aid in the development of preventive and treatment strategies.

A 10-year-old spayed female Maltese presented with purpura and hematemesis. Initial laboratory evaluation revealed immune-mediated thrombocytopenia, but evidence of hemolytic anemia was not identified. Three milligrams of human intravenous immunoglobulin (hIVIG) was administered for 3 hours following prednisolone and mycophenolate mofetil. A pale mucous membrane was identified, and the packed cell volume decreased by 3%. Blood film examination revealed significant spherocytosis with auto-agglutination. Blood transfusions and immunosuppression were continued for 4 days, and hIVIG was discontinued. This report describes a case of increased immune-mediated hemolysis after hIVIG administration, possibly due to new-onset immune-mediated hemolytic anemia or enhanced immunogenicity.

Peritoneal lymphomatosis (PL) is a rare lymphoid neoplasm in dogs. A nine-year-old spayed female Labrador retriever presented with pleural and peritoneal effusions. Diagnostic imaging revealed diffuse nodular to massive lesions in the mesentery, particularly in the caudal abdomen. While the superficial lymph nodes did not show significant changes, enlargement was observed in the intra-abdominal and intra-thoracic lymph nodes. Cytological and flow cytometric analyses of the effusion indicated the presence of large B-cell lymphocytes expressing CD3-/CD5-/CD14-/CD21-/CD34+/CD45+/CD79a+. PL was diagnosed using diagnostic imaging and fluid analysis. This case report highlights the clinical and diagnostic features of canine PL.

BACKGROUND: To accelerate the healing of diabetic wounds, various kinds of growth factors have been employed. It is the short half-life of administered growth factors in hostile wound beds that have limited wide-spread clinical usage. To overcome this limitation, growth factor gene therapy could be an attractive alternative rather than direct application of factors onto the wound beds. We administered two growth factor DNAs, epidermal growth factor (EGF) and vascular endothelial growth factor (VEGF) into a cutaneous wound on diabetic mice. We compared the different characteristics of the healing wounds. METHODS: Streptozotocin was injected intraperitoneally to induce diabetes into C57BL/6J mice. The ultrasound micro-bubble destruction method with SonoVue as a bubbling agent was used for non-viral gene delivery of EGF828 and VEGF165 DNAs. Each gene was modified for increasing efficacy as FRM-EGF828 or minicircle VEGF165. The degree of neoangiogenesis was assessed using qualitative laser Doppler flowmetry. We compared wound size and histological findings of the skin wounds in each group. RESULTS: In both groups, accelerated wound closure was observed in the mice receiving gene therapy compared with non treated diabetic control mice. Blood flow detected by laser doppler flowmetry was better in the VEGF group than in the EGF group. Wound healing rates and histological findings were more accelerated in the EGF gene therapy group than the VEGF group, but were not statistically significant. CONCLUSION: Both non-viral EGF and VEGF gene therapy administrations could improve the speed and quality of skin wound healing. However, the detailed histological characteristics of the healing wounds were different.
Animals ; DNA ; Epidermal Growth Factor ; Genetic Therapy ; Half-Life ; Intercellular Signaling Peptides and Proteins ; Laser-Doppler Flowmetry ; Mice ; Phospholipids ; Skin ; Streptozocin ; Sulfur Hexafluoride ; Vascular Endothelial Growth Factor A ; Wound Healing