24 volunteers were divided into 3 groups. Group 1 of 10 subjects was given vaccine in addition of bicarbonate damper, group 2 of 9 subjects was given vaccine and water, group 3 of 5 subjects was given bicarbonate damper. Anti LPS antibody value of V. cholerae 01 of IgA class in ELISA spot was higher significantly than serum ELISA in same time point. Lymphocyte producing IgA antibody persisted about 5 months after immunization. IgM antibody excreting cells had activated promptly rather just 7 days after the first time of talking oral vaccine with a mean value 8 times higher than the mean value before taking vaccine. 100% of studied subjects in 2 groups had had the response of IgA antibody excreted through peripheral blood lymphocytes.
Cholera Vaccines ; Lymphocytes ; Technical Report [Publication Type]

The incidence of asymptomatic malaria, non fever parasite carriers was 86.6% of total parasite carries. This incidence increased with the age and fluctuated between 84.6% and 95.5%. Among asymtomatic malaria patients, gametocyte carriers accounted for 68.5% of total gametocyte carrier number and increased with age in the 2-9 old age group, and in this group the rate of splenomegaly is higher than that of other age groups. Malarial antibody mean value in malarial asymtomatic patients proportional directly with the age and inversely proportional with parasite density. There was an association between risk factors and the asymptomatic malarial rate (OR = 2.4 and 3.0) with p<0.001.
epidemiology ; malaria ; diagnosis

Study the rapid serodiagnosis of typhoid fever in 41 samples collected at Saint Paul Hospital, Traffic-Transport Hospital, centers of preventive medicine in Hanoi, Lai Chau, Thai Binh from 2002 December to 2003 June. Results: IgM(+) and IgG(+) in 29 samples, IgM(-) and IgG(+) in 8 samples, IgM(-) and IgG(-) in 3 samples, and 1 unspecified sample. Most of typhoid patients eliminated bacteria in feces 4-12 weeks after recovery. Few patients continued elimination after 12 weeks. Typhi-Dot technique overcomes disadvantages of Widal reaction, gives quick result within 1 hour, has high sensitivity and specificity, simple technique, not requires expensive equipments, and could test many samples at the same time
Typhoid Fever ; Serologic Tests ; Diseases

The study carries on 87 E.coli strains. Using reversed passive latex agglutination (RPLA) technique to discover factor causing disease EspB to confirm EPEC. The result: 27 strains have no gene eae, 52 strains has eae possitive, 19 of them has Stx. In fact, following traditional, the rate infected EPEC was determined essentialy relying on slide agglutination reaction with antiserum group O of EPEC. This experience showed that the factor causing disease EspB in order to determine EPEC can apply at clinical laboratory or in community, although, most ETEC extract also EspB, Sxt and can also be determined in similar culture. This method is necessary need and significance in fact
Escherichia coli ; diagnosis ; Agglutination

4 strains of Vibrio cholerae 01 E1 Tor (V.cholerae 01) isolated from patients of various areas in Vietnam (20 strains in the year 1995 and 24 in 2000) and 256 strains of V.cholerae 01 isolated from various areas in Laos. (15 strains in 1993-1994 period, 21 in 1995-1996, 91 in 1998, 16 in 1999 and 109 in 2000). The ones from Laos in 1993-1996 years period were sensitive to common antibiotics such as tetracyclin, chloramphenicol, trimethoprim, but the ones of 1998-2000 were resistant to these antibiotics. The strains of V.cholerae 01 isolated in Vietnam in the year 1995 were sensitive to tetracyclin and chloramphenicol, but the ones in the year 2000 were resistant with a medium level. The strains of V.cholerae 01 in Vietnam were resistant to polymycin B, but the ones in Laos were sensitive to polymycin B
Vibrio cholerae ; Pharmaceutical Preparations ; epidemiology ; drugs

The random amplified polymorphic DNA (RAPD) technique was used to distinguish 60 strains of Vibrio cholerae 01 E1 Tor isolated from patients in 1991-2002 period. There were 6 strains isolated from HoChiMinh city, 43 strains from Nha Trang, 8 strains from Hanoi and 3 strains from Tay Nguyen Highland. In RAPD technique, DNA oligomers with 10 nucleotids was selected randomly using to synthese prime DNA from the sites of properly paired aminoacids. Strain specific amplified DNA was created. AP42 and AP46 were used to identify the gene of various strains in diverse areas. Results of RAPD showed that these strains of bacteria had a same origin. RAPD technique can be used to supervise the transmission of bacteria in human
DNA ; Random Amplified Polymorphic DNA Technique ; Vibrio cholerae ; DNA Fingerprinting

ELISA technique was used to determine promtly the specific antibodies in equine sera. The results were comparable to these of mouse neutralization test. This method was recommended to monitor the antibody response in horse and to determine the titers of rabies antibody
Rabies ; Enzyme-Linked Immunosorbent Assay ; Diseases

The incidence of cerabral paralysis in Ha Tay Province is counted by 2 ways: the incidence on lower 5 years old children an “the incidence on newborn”. The incidence on children who were borned from 1/1/1994 to 31/12/1997 live to 5 years old is 3.01/1000 in which the highest years (1996) is 4.69/1000 and the lowest years (1994) is 1.97/1000. Rate of getting cerebral paralysis take 1/3 new cases at the same age group. Rate of death at ≤ 5 age take 1% nearly (112/1620). The rate include death in newborn. Number of ≤ 5 children death due to cerabral paralysis take 11% death cases total. New suffer cerabral paralysis rate from 1994-1997 is 4,18/1000 alive children
Paralysis ; Brain Ischemia ; epidemiology

Grayanatoxin IV, a tetracyclic diterpenoid, was isolated from the leaves of Macaranga triloba for the first time. Its structure was elucidated on the basis of spectral analysis including1H-NMR,13CNMR and 2D-NMR techniques in comparison with 13CNMR data of the authentic compound
Pharmacy ; plants ; Plant Leaves ; drugs

The results of Danver test and Raven test on 238 > 6 years old of age children with cerebral palsy in HaTay province were assessed. With Ravent test, only 2% among them had not mental retardation, but 98% had mental retardation in various levels. Among the subjects of floppy form of cerebral palsy there is not any case of normal mental level, but a light level of retardation. With Denver test, 26% of cerebral palsy children had got normal development in comparing with the other. But there are different levels of retardation in various domains, 97% of cerebral palsy children got retardation in mobility domain, 85% in individual and social domain, 61% in adaptative mobility and 53.7% in laguage domain
Cerebral Palsy ; Brain ; Brain Diseases