1.Technique of converse latex passive agglutination in the diagnosis of pathogenic escherichia coli
Journal of Practical Medicine 2003;459(9):41-42
The study carries on 87 E.coli strains. Using reversed passive latex agglutination (RPLA) technique to discover factor causing disease EspB to confirm EPEC. The result: 27 strains have no gene eae, 52 strains has eae possitive, 19 of them has Stx. In fact, following traditional, the rate infected EPEC was determined essentialy relying on slide agglutination reaction with antiserum group O of EPEC. This experience showed that the factor causing disease EspB in order to determine EPEC can apply at clinical laboratory or in community, although, most ETEC extract also EspB, Sxt and can also be determined in similar culture. This method is necessary need and significance in fact
Escherichia coli
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diagnosis
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Agglutination
2.Transition of drug susceptibility of vibrio cholerae 01 in Laos and Vietnam
Journal of Preventive Medicine 2003;13(4):27-31
4 strains of Vibrio cholerae 01 E1 Tor (V.cholerae 01) isolated from patients of various areas in Vietnam (20 strains in the year 1995 and 24 in 2000) and 256 strains of V.cholerae 01 isolated from various areas in Laos. (15 strains in 1993-1994 period, 21 in 1995-1996, 91 in 1998, 16 in 1999 and 109 in 2000). The ones from Laos in 1993-1996 years period were sensitive to common antibiotics such as tetracyclin, chloramphenicol, trimethoprim, but the ones of 1998-2000 were resistant to these antibiotics. The strains of V.cholerae 01 isolated in Vietnam in the year 1995 were sensitive to tetracyclin and chloramphenicol, but the ones in the year 2000 were resistant with a medium level. The strains of V.cholerae 01 in Vietnam were resistant to polymycin B, but the ones in Laos were sensitive to polymycin B
Vibrio cholerae
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Pharmaceutical Preparations
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epidemiology
;
drugs
3.PCR - based random amplified polymorphic DNA fingerprinting of vibrio cholerae 01 in Vietnam
Journal of Preventive Medicine 2003;13(5):27-30
The random amplified polymorphic DNA (RAPD) technique was used to distinguish 60 strains of Vibrio cholerae 01 E1 Tor isolated from patients in 1991-2002 period. There were 6 strains isolated from HoChiMinh city, 43 strains from Nha Trang, 8 strains from Hanoi and 3 strains from Tay Nguyen Highland. In RAPD technique, DNA oligomers with 10 nucleotids was selected randomly using to synthese prime DNA from the sites of properly paired aminoacids. Strain specific amplified DNA was created. AP42 and AP46 were used to identify the gene of various strains in diverse areas. Results of RAPD showed that these strains of bacteria had a same origin. RAPD technique can be used to supervise the transmission of bacteria in human
DNA
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Random Amplified Polymorphic DNA Technique
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Vibrio cholerae
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DNA Fingerprinting
4.Enzyme linked immunosorbent assay for rapid quantitatin of rabies antibodies
Journal of Preventive Medicine 2003;13(6):71-74
ELISA technique was used to determine promtly the specific antibodies in equine sera. The results were comparable to these of mouse neutralization test. This method was recommended to monitor the antibody response in horse and to determine the titers of rabies antibody
Rabies
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Enzyme-Linked Immunosorbent Assay
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Diseases
5.Some observation on the incidence of cerebral paralysis in HaTayprovince
Journal of Practical Medicine 2003;463(10):54-55
The incidence of cerabral paralysis in Ha Tay Province is counted by 2 ways: the incidence on lower 5 years old children an “the incidence on newborn”. The incidence on children who were borned from 1/1/1994 to 31/12/1997 live to 5 years old is 3.01/1000 in which the highest years (1996) is 4.69/1000 and the lowest years (1994) is 1.97/1000. Rate of getting cerebral paralysis take 1/3 new cases at the same age group. Rate of death at ≤ 5 age take 1% nearly (112/1620). The rate include death in newborn. Number of ≤ 5 children death due to cerabral paralysis take 11% death cases total. New suffer cerabral paralysis rate from 1994-1997 is 4,18/1000 alive children
Paralysis
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Brain Ischemia
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epidemiology
6.Tetracyclic diterpene from Macaranga triloba (Blume) Muell-Arg
Pharmaceutical Journal 2003;328(8):18-20
Grayanatoxin IV, a tetracyclic diterpenoid, was isolated from the leaves of Macaranga triloba for the first time. Its structure was elucidated on the basis of spectral analysis including1H-NMR,13CNMR and 2D-NMR techniques in comparison with 13CNMR data of the authentic compound
Pharmacy
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plants
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Plant Leaves
;
drugs
7.The results of Denwer and Raven test in 238 cerebral palsy patients
Journal of Practical Medicine 2003;466(11):21-24
The results of Danver test and Raven test on 238 > 6 years old of age children with cerebral palsy in HaTay province were assessed. With Ravent test, only 2% among them had not mental retardation, but 98% had mental retardation in various levels. Among the subjects of floppy form of cerebral palsy there is not any case of normal mental level, but a light level of retardation. With Denver test, 26% of cerebral palsy children had got normal development in comparing with the other. But there are different levels of retardation in various domains, 97% of cerebral palsy children got retardation in mobility domain, 85% in individual and social domain, 61% in adaptative mobility and 53.7% in laguage domain
Cerebral Palsy
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Brain
;
Brain Diseases
8.Evaluation of efficacy of rigevidon in treating pubertal menorrhagia at Institute for Protection of Mother and Newborn
Journal of Practical Medicine 2005;510(4):91-92
Study on 80 symptomatic menorrhagia patients less than 18 years old treated at Institute for Protection of Mother and Newborn in the year 2000. Results: the rate of patients with irregular menstruation was highest (38.75%). 70% of patients lived in rural, so the rate of late hospital admission (>15 days) accounted for 73.75%. All patients suffered from anemia with 82.5% patients with moderate and severe anemia. Treatment with 2 tablets Rigevidon a day only one time in the evening until menorrhagia reduces, follows by 1 tablet a day for making artificial menstruation of 28 days, is effective in 96.25% patients. This finding showed that using Rigevidon for treating pubertal menorrhagia is simple, safe, and effective, no significant complication.
Menorrhagia
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Ethinyl Estradiol-Norgestrel Combination
;
Therapeutics
9.The situation of nutrition and eating habit of resident students in Thai Nguyen Medical College in the new period
Journal of Practical Medicine 2005;505(3):96-99
A cross-sectional study on the situation and eating habit of 412 resident students at the age from 21.3 ± 1.1 (60% were males, 40% were females) were conducted in Thai nguyen Medical College from Oct 2003 to Feb 2004. The result showed that: the propotion of persistent malnutrition was 20.4%, in which male: 10.9%, female: 34.5%. Overweight: 1%. The portion for each student seem to be more fufil with meat, fish and vegetable both in qualities and to comparision with the year of 1980. The evarage enegry in each portion is 2244.8 kcal, reach 93.0% to comparision of request for male and 84.0% for female. The balance rate P:L:G is 16:22:62. The habit relating to nutrition: nosh OR=3.1, none breakfast OR=8.81. There is no relation between the nutrition situation with expense norm for meals and some other habits such as un stable time, cooking by themselves.
Nutritional Status
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Eating
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Students
10.Study on hemo-activating effects of the prescription "tø vËt ®µo hång" in experimental tests
Pharmaceutical Journal 2003;9(6):24-26
The remedy "Tu vat dao hong" is made up and manufactured at Pharmacy in Army Traditional Medical Institute. The experiment is conducted on healthy rabbits after coagulating endovascular scattered blood in 3 groups (treated group, controlled group, tanakan group). Results: Howell time in all groups were shorted, rabbits died at second week in controlled group; Quick time were also shorted, and rabbits died also at second week; APTT time reduced significantly (p<0.05) when compared with previous experiment. Platelet quantify, fibrinogen quantify, ethanol test were limited. The remedy have good relative effects when compared tanakan standard drug used in same condition
Prescriptions, Drug
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Pharmacy
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Medicine, Traditional