Cultured T.vaginalis was used for the anti-trichomonas test at different times,concentrations of propolis and densities of the parasites.After being cultured for 0,6,12,24 hrs,the survival rate of the parasites was(91.50?3.11)%,(43.00?6.83)%,(22.25?5.32)% and(11.50?5.74)% respectively with a significant difference between propolis group and the control.Under the concentrations of 0.24,0.48,0.96,1.92,3.84 and 7.68(mg/ml) with 24 hrs culture,the survival rate was(88.00?5.29)%,(92.67?4.16)%,(90.0?6.00)%,(84.00?4.00)%,(2.67?1.15)%,and 0 respectively.The results showed that propolis possesses clear in vitro anti-trichomonas activity which is relevant to the duration of culture and the concentration of the agent.

A thin layer chromatograph-fluorescent method was used to assay content of scopoletin in Erycibe obtusi folia Benth.. The method showed that it not only can minimize analytical errors in thin layer scan and column contamination in HPLC,but also eliminated mutual interference of components in the preparation. It provides a good assay technique for quality control of hebal preparations. The method was carried out on thin layer plates of silica gel H(40?)with benzene-ether (1. 1: 1 )as the developer,at an excitation spectrum of 347nm,fluorescence spectrum of 420nm and a temperture of 25??1℃.The method showed good linearity(r= 0. 9998, n = 5)in the range of 0.08~0. 40?g/?l of scopoletin and the average recovery was 98. 52 % ~ 102. 67 % with RSD

Objective To study the inhibit efficiency of lake sediment phosphorus release by adding chemical reagents and to provide the technical basis for the comprehensive management of city lake.Methods In May 20,2008,Mochou Lake upper sediment and water samples were collected.The phosphorus forms of sediments,total phosphorus(TP),NaOH-P,HCl-P,inorganic phosphorus(IP),organic phosphorus(OP) were analyzed and the pH value and content of TP in water samples were determined.Each water(250 ml)-sediment(10 g) system was respectively added by 0-1 000.0 mg/L of Ca(OH)2,2.0-10.0 mg/L of A12(S04)3 and 0-25.0 mg/L of FeCl3.The pH value and content of TP in water were determined,then the control rate of TP release were calculated.Results The pH values were between 7.6-8.0,TP content was 2.0 mg/L in water of Mochou Lake.The contents of TP,HCl-P,NaOH-P,IP,OP were 2 187.06,1 383.89,526.48,1 910.37 and 276.69 mg/kg respectively.With the increase of Ca(OH)2 concentration,pH value of water increased rapidly,content of TP showed a decreasing trend,control rate of TP release was increased.With the increase of A12(SO4)3 or FeCl3 concentration,pH value of water declined slightly,content of TP showed a decreasing trend,control rate of TP release was increased.Conclusion Ca(OH)2,A12(SO4)3,FeCl3 can significantly inhibit the phosphorus release of sediments in Mochou Lake.

Objective To explore the effect and utilization of comprehensive treatment on intrahepatic recurrence after hepatic resection for hepatocellular carcinoma.Methods One and two year recurrent rate of 60 patients with hepatocellular carcinoma who underwent hepatic resection in a two-year follow up were analyzed retrospectively,control group 30 cases,comprehensive treatment group 30 cases.Results One and two year intrahepatic recurrent rate of comprehensive treatment is 13％(4/30)and 40％(12/30)respectively.The difference was statistically singnificant(x2=4.176,4.310,all P＜0.05).Conclusion The effect of comprehensive treatment on postponing intrahepatic recurrence after hepatic resection for hepatocellular carcinoma is obvious.

A postulated suppression of T lymphocyte function by clonidine (an adrenogic receptor agonist) was explored by utilizing mitogen induced lymphocyte transformation of mouse spleen cells in vitro. Added at the onset of the culture or 24h later, clonidine (10-(?)-10-8 M ) did not suppress T lymphocyte transformation induced by ConA or PHA. It implies that there is a difference in the sensitivity for clonidine between T and B lymphocyte, as clonidine has been suggested to suppress the SRBC induced primary immune response of mouse spleen cells in a dose dependent manner.

0.05).In comparison with pre-treatment,WOMAC scores,pain index,and physiological integral scores in treatment and control groups after the first and the last treatment decreased significantly(P

OBJECTIVE To analyze the cleaning and disinfecting effect of digestive endoscope by the Mall disinfection center for endoscopes.METHODS Fifty-six pieces of endoscopes were cleaned and disinfected by the Mall disinfection center,and checked randomly. An other 50 endoscopes were treated as control group.All pieces were taken the biological inspection.RESULTS The qualification rate of lumen disinfection was 100% in test group,and 92% in control group(P

Objective To explore the diagnosis evaluation of 64-slice spiral CT angiography(CTA) in cerebral vascular disease.Methods 64-slice spiral CT angiography of 48 patients with suspected clinically cerebral vascular diseases were analyzed retrospectively.The source images were got by TOSHIBA Aquilion VCT Scanner,and all axial images were transferred to an external workstation.The reconstructed images were processed into volume rendering(VR),multiplanner reconstruction(MPR) and maximum intensity projection(MIP),the values were valuably compared with the results of surgery or DSA.Results 30 cases were aneurysms,4 cases were AVM,8 cases were cerebral artery stenosis or occlusion,6 cases were normal.Conclusion The 64-slice spiral CTA is a valuable diagnostic method for various cerebral vascular diseases.

OBJECTIVE To enhance the standardized management for cleaning and disinfection of endoscope,and prevent hospital infection due to endoscope examination and treatment.METHODS The main measured were established and consummated the system,increased the investment of facilities,strengthened personal training and standardized the cleaning and disinfection work procedures.RESULTS The endoscopes after disinfection were all reached the standards.CONCLUSIONS The standardized management for cleaning and disinfection of endoscope is the assurance for the endoscope sterilization reaching the standards.

Mechanism of Immunomodulatory actions of total glucosides of paeony ( TGP ) was studied by McAb indirect immunofluorescence. TGP 5 mg/( kg?d ) ip for 8d suppressed DTH, that was enhanced by cyclophosphamide( Cy), and decreased L3T4+/Lyt-2+ 〔 (Th/i)/(Ts /c) 〕 cell ratio in ICR mice .TGP 5 mg/ ( kg?d ) ip for 5d enhanced DTH that was suppressed by Cy, and increased the L3T4+/Lyt-2 + cell ratio in ICR mice. The same dose of TGP ip for 5d promoted the hemolysin production of spleen cells, that was suppressed by Cy, increased the L3T4+/Lyt-2 + cell ratio in BLAB/c mice. The results suggest that immunomodulatory actions of TGP might be brought about by altering Th/Ts cell ratio.