Objective: To investigate the peripheral blood mucosa-associated constant T cell(MAIT) expression in children with bronchial asthma and its relevance to severity. Methods: Ninety-eight children with bronchial asthma who were treated in Affliated Dongfeng Hospital from May 2016 to May 2019 were selected as the asthma group. Another 60 healthy children who underwent health checkup during the same period were as control group. The levels of MAIT were detected by flow cytometry analyzer using BD FACSAria II flow cytometry, and the lung function was detected by Yage lung function tester. The receiver operating characteristic (ROC) curve was used to evaluate the diagnostic efficacy of peripheral blood MAIT for bronchial asthma. Pearson analysis was used to examine the relationship between MAIT cells and pulmonary function. Results: There were no significant differences in the general data of gender, age, and body mass index between the two groups (P>0.05). The asthma control test (ACT) score, the percentage of first second forced expiratory volume occupied estimated value (FEV₁%), the percentage of first second forced expiratory volume occupied forced vital capacity (FEV₁/FVC), the percentage of peak expiratory flowoccupied estimated value (PEF), and MAIT cells in asthma group were significantly lower than those in control group (P<0.05). Serum MAIT identified bronchial asthma and healthy controls with an area under curve (AUC) of 0.900, with a sensitivity of 82.65%, and a specificity of 85.00%. Serum MAIT identified the mild-sustained group and the healthy control group with an AUC of 0.910, with a sensitivity of 94.64%, and a specificity of 81.67%. Correlation analysis showed that MAIT cells in children with bronchial asthma were significantly positively correlated with pulmonary function indexes such as FEV₁%, FEV₁/FVC and PEF (r=0.159, 0.222, 0.213, P<0.05). Conclusions The peripheral blood MAIT in children with bronchial asthma is significantly reduced, which is related to the levels of lung function indicators. MAIT can be used as one of the indicators for the diagnosis and clinical prognosis of patients with bronchial asthma.

Objective: To investigate the diagnostic and predictable value of the levels of serum Trx (S-Trx), urinary Trx (U-Trx) and S-Trx/U-Trx ratio in acute pyelonephritis of children. Methods: A total of 120 children with urinary tract infection were divided into APN group (67 cases) and non-APN group (53 cases).In addition, 67 children with APN were assigned to severe group (23 cases)and non-severe group (44 cases). The leves of serum C-reactive protein(CRP), precalcitonin(PCT), cystatin C (CysC), Trx (S-Trx) and urinary β₂-microglobulin(β₂-MG), neutrophill gelatinase-related apolipoprotein (NGAL), Trx (U-Trx) were collected. Besides, the ratio of S-Trx/U-Trx was also counted. The diagnostic and predictable value of each index were determined by receiver operating characteristic (ROC). Results: Serum PCT, S-Trx, urinary β₂-MG, NGAL, U-Trx was markedly increased and S-Trx/U-Trx ratio was obviously decreased in the APN group compared to that of the non-APN group (P<0.05). Moreover, S-Trx, urinary NGAL, U-Trx was significantly increased and S-Trx/U-Trx ratio was significantly decreased in the severe group compared to that of the non-severe group (P<0.05). Both in APN group or severe APN group, S-Trx and U-Trx displayed significantly positive correlation (P<0.05). The results from ROC demonstrated that AUC were 0.960, and optimal cut-off value were 16.17 μg/L of U-Trx diagnostic APN in children, with a specificity and sensitivity of 94.34% and 92.54%, respectively. The optimal cut-off value was 1.29.AUC of S-Trx/U-Trx 0.960 predicted the severe of APN in children, with a specificity and sensitivity of 81.82% and 82.61%, respectively. Conclusions U-Trx is useful for diagnostic APN in children, S-Trx/U-Trx is an independent predictor of the severe APN.

Objective To reveal the mechanism of action of AS-Ⅳ on HepG2 cells based on molecular dynamics simulation and experimental evaluation. Methods We constructed a "drug-disease" network pharmacological map, analyzed the core genes of astragaloside Ⅳ (AS-Ⅳ) in HCC, screened key signaling pathways, and established a "drug-target" molecular dynamics model. In vitro assay was used to detect migration, proliferation and invasion abilities. Flow cytometry and qRT-PCR were used to detect the effect of AS-Ⅳ on the cell cycle and apoptosis, and the expression of core gene of HepG2. Results The core target of AS-Ⅳ acting on HCC was VEGFA. Compared with the control group, the high concentration of AS-Ⅳ significantly inhibited the migration, invasion and proliferation of HepG2 cells, blocked the metastasis of HepG2 cells from G₁ to G₂ phase, promoted their apoptosis, down-regulated VEGFA expression and up-regulated TGF-β1 expression. Conclusion AS-Ⅳ may inhibit the proliferation of hepatocellular carcinoma cells through multi-target and multi-pathway.