BACKGROUND: Continuous oxygen inhalation of high concentration may improve antioxidase and simultaneously injure the lung in rats.OBJECTIVE: To observe the effect of intermittent high concentration oxygen on antioxidase activity and pulmonary tissue in rats.DESIGN: Complete randomized control experiment.SETTING: Department of Geriatric Cardiology and Department of Biochemistry, General Hospital of Chinese PLA. MATERIALS: The experiment was performed in Department of Hyperbaric Oxygen of Navy General Hospital and Department of Biochemistry of General Hospital of Chinese PLA from January 2002 to May 2002, in which, 10 healthy Wister rats were employed, mass weighted varied from 200 to 250 g, of either sex and randomized into two groups, 5 rats in each.METHODS: In the control, the rats were bred in the room environment for 7 days. In hyperoxic preconditioning (HP) group, the rats were placed in hyperbaric oxygen chamber and inhaled oxygen of 80％ to 85％ (101. 325 kPa) everyday for 6 hours, continuously for 7 days. At the end of experiment, the lung was collected to measure lung wet mass. 0.4 g pulmonary tissue was collected to prepare homogenate in freezing bath and its upper clear solution was collected to determine the activities of superoxide dismutase (SOD), glutathione peroxidase (GTTP) and catalase with chemiluminescence method successively. Pulmonary tissue was fixed with 30 g/L glutaradldehyde and prepared into sections to be observed the microstructure of lung under electron microscope.MAIN OUTCOME MEASURES: ① Comparison of lung wet mass and activities of SOD, glutathione peroxidase and catalase in pulmonary tissue in two groups. ② Alternation on microstructure of pulmonary tissue.RESULTS: Ten rats were all entered result analysis. ① Activities of SOD,GTTP and catalase in pulmonary tissue: Those in HP group were all higher remarkably than the control [(185.10±1.92) ％/g, (90.50±4.17) nkat/g,(4.38±0.18) k/g; (95.15±1.43) ％/g, (20.17±2.17) nkat/g, (1.10±0.19) K/g,t= -84.016, -33.488, -28.023, P ＜ 0.01]. ② Lung wet mass: That in HP group was near to the control [(1.32±0.07), (1.30±0.03) g, P ＞ 0.05], explaining that the fluid in the lung of HP group was not increased. ③ Microstructure of pulmonary tissue: In HP group, the basement membrane of pulmonary tissue was integrative and thickness was even, vascular endothelial cell was integrative and Ⅰ-type epithelial cell was not destroyed.CONCLUSION: Intermittent high concentration oxygen improves antioxidase activity in the lung of rat and does not injure the lung obviously.

Objective To investigate the expression of carbohrdyate antigen (CA153 ) and carcinoembryanic antigen(CEA) ,and itsclinical significance in the patients with breast cancer.Methods The sera CA153,CEA levels were determined by IMX in 30 healthypersons,52 patients with benign breast diseases and 208 patients with breast cancer from 1998 to 2002 ,the correlation between the CA153,CEA level and the clinical characteristics was also detected.Results In the patients with breast cancer, the level of serum CA153 and CEA was significant higher in pre-treatment than that in post-treatment(P

OBJECTIVE To understand the application value of protective specimen brush(PSB)in severe pneumonias.METHODS Fifty seven cases diagnosed as severe pneumonia were admitted into the group.The specimens were sampled via the fiberoptic bronchoscope(FOB)and the PSB and then were done bacteria quantitative culture(QC).According to these,we adjusted antibiotics.RESULTS With the PSB and bacteria QC technologies,45 strains were detected in 57 cases,with a positive rate of 78.95%.In the progress of operating FOB-PSB examination,patients life signs didn't change significantly.FOB-PSB's sensitivity and specificity were about 97.73% and 84.62%,respectively.The therapy efficiency rate was 91.23%.CONCLUSIONS It is a good choice to use the PSB for sampling in severe pneumonia via fiberoptic bronchoscope.

OBJECTIVETo observe the effect of rosiglitazone (RGZ) on the mRNA expression of HIF1α and IGF1 genes in the myeloma cells and explore possible mechanism of angiogenesis inhibition.

METHODSHuman myeloma cell line RPMI8226 and primary myeloma cells from five patients enriched by using CD138 immunomagnetic beads were treated with different concentrations (10, 20, 40 μmol/L) of RGZ. The mRNA expression of HIF1α and IGF1 was analyzed in cells treated with RGZ after 48h by RT-PCR, The levels of phosphorylated AKT and ERK proteins were detected by Western blotting. Bone marrow mononuclear cells from five patients with iron deficiency anemia were regarded as control.

RESULTSHigher mRNA expression of HIF1α and IGF1 genes in RPMI8226 and in primary myeloma cells was showed as compared to those in control. Treated with RGZ of 20 μmol/L after 48 h, the mRNA expression of HIF1α (1.21 ± 0.08 vs 0.75 ± 0.06) and IGF1 (0.62 ± 0.06 vs 0.32 ± 0.04) in RPMI8226 cells was declined as compared to those without RGC treatment. The same declination was also seen in primary myeloma cells (HIF1α: 2.02 ± 0.16 vs 0.53 ± 0.04; IGF1: 1.92 ± 0.13 vs 0.58±0.03). RGZ could inhibit the expression of pAKT and pERK, nor the total AKT and ERK proteins, in RPMI8226 cells in a dose-dependent manner at the concentration of 10 μmol/L, 20 μmol/L, and 40 μmol/L.

CONCLUSIONRGZ could inhibit the mRNA expression of HIF1α and IGF1. Inhibition of angiogenesis by RGZ may be associated with down-regulation of pAKT and pERK expression.

Cell Line, Tumor ; Extracellular Signal-Regulated MAP Kinases ; metabolism ; Gene Expression Regulation, Neoplastic ; Humans ; Hypoxia-Inducible Factor 1, alpha Subunit ; genetics ; metabolism ; Insulin-Like Growth Factor I ; genetics ; metabolism ; Multiple Myeloma ; blood supply ; metabolism ; Neovascularization, Pathologic ; Proto-Oncogene Proteins c-akt ; metabolism ; RNA, Messenger ; genetics ; Thiazolidinediones ; pharmacology