Objective:To investigate the expression and clinical significance of CENPF in renal clear cell carcinoma(KIRC).Methods:The UALCAN and HPA databases were applied to analyze the difference in expression of CENPF in KIRC compared with paracancerous tissue the relationship between CENPF gene and clinicopathological parameters and prognosis of KIRC patients were analyzed by the GEPIA database;the relationship between CENPF and immune infiltration level of KIRC were analyzed by the TIMER database;the UALCAN database was used to analyze the co-expression network of CENPF genes in TCGA KIRC patients,GO enrich-ment analysis and KEGG enrichment analysis were performed on the basis of the co-expression network.CCK-8,Transwell and clone formation assays were used to investigate the effect of CENPF down-regulated expression in renal cancer cell lines on the proliferation,migration and clone formation of ACHN and 786-O cells.Results:CENPF expression was significantly highly expressed in KIRC pa-tients.High CENPF expression was negatively correlated with overall survival(OS)and disease-free survival(DFS).The expression of CENPF in KIRC patients were significantly and positively correlated with tumor cell purity,macrophages,CD8+T cells,CD4+T cells,neutrophils and dendritic cells in tumor microenvironment.The correlation network analysis indicated that CENPF may be in-volved in the development of KIRC through the regulation of cell cycle,cell mitosis and DNA repair.In addition,CENPF mRNA ex-pression was also increased in renal cancer cell lines.CCK-8 assay showed that CENPF knockdown inhibited the proliferation of ACHN and 786-O cells.Transwell assay results indicated that CENPF knockdown suppressed the migration of KIRC cells.The results of cell clonal formation experiment showed that interference with CENPF gene expression inhibited the clonal formation ability of 786-O cells.Conclusion:This study suggests that CENPF plays a key role in the progression of KIRC.CENPF has prospective clinical sig-nificance as a prognostic indicator and potential target of KIRC.