Objective To study changes and meaning of serum IL -4,IL -10,IFN -gamma,TGF -beta 1 level in patients with delayed encephalopathy after carbon monoxide poisoning.Methods From January 2011 and June 2014 in our hospital,40 cases of DEACMP patients were selected as group A;the 40 patients with DEACMP was divided into 12 cases of mild cognitive dysfunction,mild cognitive dysfunction in 20 cases,8 cases of severe cognitive dysfunction.40 cases with acute carbon monoxide poisoning (ACMP)patients were selected as group B;40 cases of healthy subjects were selected as healthy controls.Serum interleukin 4 and interleukin 10(IL -4)(IL -10),interfer-on gamma (IFN -gamma),conversion,growth factor beta 1 (TGF -beta 1)content were tested.Results In Group A and group B serum IL -4 levels were lower than that of healthy controls (P <0.01),and serum levels of IL -10 in group B were higher than that in group A and healthy controls (P <0.01),serum level of IFN -gamma in group A and group B was higher than the healthy controls (P <0.01),and in group A IFN -gamma levels were higher than group B (P <0.01),while in group A serum TGF -beta 1 level was lower than B group and the control group (P <0.01),and in group B TGF -beta 1 level was higher than the control group (P <0.01);With cognitive impairment aggravating of DEACMP patients,IL -4,IL -10,TGF -beta 1 levels were on the decline,and IFN -gamma level was increasing (P <0.01).Conclusion DEACMP patients serum and cerebrospinal fluid of IL -4,IL -10,IFN -gam-ma,TGF -beta measures such as abnormal expression,showed that the onset of DEACMP may be related to neural immune injury.Serum IL -4,IL -10,IFN -gamma and TGF -beta of DEACMP patients exists abnormal expression Serum IL -4,IL -10,IFN -gamma and TGF -beta levels are also related to the degree of cognitive impairment of DEACMP patients.The onset and progress of DEACMP may be associated with neural immune injury.

Objective To examine the effects of weight loss on functional state of female judokas. Methods Twelve female judokas belonging to Shanghai female judo team were tested at different periods(first,second,third phases and last phase) during weight loss a month before competition. The changes of physiological and biochemical indexes in different periods were observed and all the data was statistically analyzed. Results Previous three phases,the Fat% in weight loss group was decreased compared with basic data (P

Objective To explore the effects of vascular endothelial growth factor (VEGF) and nitric oxide (NO) on coronary collateral blood flow (CCBF) in a rabbit model of physical ischemic training (PIT),and to provide a foundation for the study of the mechanism of collateral circulation formation in PIT.Methods Models of intermittent myocardial ischemia were established in 55 rabbits by installing a water balloon constrictor on the left ventricular coronary artery.The rabbits were then divided into a sham-operation group,a pure ischemia group,a PIT group,a PIT group with NO inhibition pretreatment,a PIT group with VEGF inhibition treatment,and a PIT group with both NO and VEGF inhibition pretreatment.Microspheres were injected at modeling and before sacrifice before and after ischemic stimulation.At the termination of the experiment the ischemic myocardial tissue of the left ventricular anterior wall was sampled,and then relative CCBF was measured using the microspheres.Results There was no significant difference among the 6 groups before training.After training prominent differences among the groups were observed,with the PIT group having the greatest increase in CCBF.CCBF in the pure ischemia group,the PIT group,and the PIT group with NO inhibition pretreatment were significantly higher than in the sham-operation group.Significant differences were also found between the PIT group and the three pretreatment groups.CCBF in the PIT group with NO pretreatment and VEGF inhibition was significantly different from that in the NO pretreatment group,while it was not found in the group with VEGF inhibition pretreatment.The CCBF in the group with VEGF inhibition pretreatment and the group with NO inhibition pretreatment was similar.Conclusions PIT can improve CCBF to the remote ischemic myocardium when combined with VEGF and NO pretreatment.Blocking VEGF or NO can reduce CCBF after training,and both VEGF and NO are involved in the formation of collateral circulation,with VEGF playing the major role.

Objective To investigate the effect of donepezil hydrochloride on the expression of Calpain Ⅰ-Cdk5/p25 pathway in the hippocampal CA1 area by cerebral ischemia and reperfusion in mice.Methods Mice were divided into model group,sham-operated group and donepezil-treated group.The expression of Calpain Ⅰ in hippocampal CA1 area was measured by immunohistochemistry staining respectively at 4,6 and 8 weeks post cerebral ischemia and reperfusion.Western blot was used to evaluate Cdk5 and p25 protein expression.Results The abilities of learning and memory performance was damaged significantly at 4,6 and 8 weeks after surgery compared to sham-operated group (P＜ 0.05).The expression of Calpain Ⅰ of model group were (0.098 ± 0.009),(0.129 ±0.01),(0.116 ± 0.01),which were higher than that of sham-operated group (0.03 ± 0.003),(0.031 ± 0.003),(0.029 ±0.003) and there was significant difference (P ＜ 0.05).The expression of Cdk5 in model group was (0.54 ± 0.05),(0.73 ± 0.07),(0.7 ± 0.06),which were higher than that of sham-operated group (0.23 ±0.02),(0.31 ± 0.02),(0.33 ± 0.02) and there was significant difference (P ＜ 0.05).The expression of p25 in model group was (0.44 ± 0.04),(0.51 ± 0.04),(0.55 ± 0.06),which were higher than that of sham-operated group(0.19 ± 0.02),(0.24 ± 0.02),(0.2 ± 0.02) and there was significant difference (P ＜ 0.05).The expression of Calpain Ⅰ of donepezil-treated group was (0.041 ± 0.004),(0.054 ± 0.004),(0.046 ± 0.003),which were lower than that of model group.The expression of Cdk5 was (0.28 ± 0.02),(0.33 ± 0.03),(0.38 ± 0.02),and expression of p25 was (0.26 ± 0.02),(0.25 ± 0.03),(0.21 ± 0.02),which were lower than that of model group respectively(P ＜ 0.05).Conclusion Donepezil hydrochloride probably improve the learning and memory abilities by reducing the expression of Calpain Ⅰ and Cdk5/p25.

Objective The purpose of this study is to study the expression of miR -221 in cervical cancer tissues and its relationship with HPV infection .Methods HR-HPV infection was detected by HC2,and 30 cases of HR-HPV negative and 5 cases of HR-HPV positive cervical cancer tissues were collected .Mean-while,30 cases of normal cervical tissues in patients with benign disease were collected as control group .The ex-pression of miR -221was detected by RT -PCR,preliminarily investigating the relationship between miR -221 expression and the occurrence of cervical cancer and HPV infection .Through transfection of miR-221 and anti-miR-221 into HPV16-positive cervical carcinoma cell line Caski and HPV 16-negative cervical carcinoma cell line C33a,we observed the role of miR -221 on the migration and invasion of Caski cells and C 33a cells.Results Compared with normal cervical tissues , the expression of miR -221 in cervical cancer was significantly in-creased,the difference was statistically significant( P<0.01);and the expression of miR-221 was closely correl-ative to the patients with or without lymph node metastasis ,pathological grade and clinical stage ( P<0 .01 );the expression of miR-221 in HR-HPV positive cervical cancer tissues was higher than in HR -HPV negative cer-vical cancer tissues(P<0.01);transfection of miR -221 and anti-miR-221 could promote or downregulate C33 a and Caski cells migration and invasion ,and the changes between two groups had statistical significance ( P<0.05).Conclusion The increased expression of miR -221 in cervical cancer tissues is closely related to the oc-currence and development of cervical cancer and HPV infection .

Objective To compare the anti-tumor activity and antioxidant activity of ethanol extracts of F.veluties and A.auricula.Methods Three human carcinoma cell lines, including MCF-7, HeLa and A375 were assessed by MTT assay to measure cell viability.The antioxidant activity was detected with a DPPH and RFAP assays.Results Two domestic fungus have different anti-tumor activity on the inhibition of MCF-7, HeLa and A375 cells at 25-400μg/mL concentration rage.F.velutipes was better than the A.auricula.The difference was statistically significant ( P<0.05 ).Compared with the control group, the inhibition of F.velutipes were 48.20%, 52.61%and 50.58%at the concentration of 400μg/mL, respectively (P<0.01).At the same concentration, the inhibition of A.auricula were 37.62%、50.21%and 41.59%, respectively (P<0.01).The ethanol extracts of two domestic fungus have significant antioxidant activity.F.velutipes was better than the A.auricula.The difference was statistically significant ( P<0.01 ).Compared with the control group, the scavenging radical capacities on DPPH of ethanol extracts of F.velutipes and A.auricula were 60.30%and 40.43%at the concentration of 1.6 mg/mL, respectively (P<0.01).At the same concentration FRAP value were 9.5 and 7.0 mmol/mL(P<0.01).Conclusion The F.velutipes and A.auricula both have strong anti-tumor and antioxidant activities, F.velutipes is better than A.auricula.

A novel high performance liquid chromatographic method was developed for the determination of 4-O- methylhonokiol in rabbit plasma and was applied to its pharmacokinetic investigation. Plasma samples were treated by one-fold volume of methanol and acetonitrile to remove the interference proteins. A reverse phase column of SHIM- PACK VP-ODS （150 mm ×4.6 mm, 5.0 μm） was used to separate 4-O-methylhonokiol in the plasma samples. The detection limit of 4-O-methylhonokiol was 0.2 μg/L and the linear range was 0. 012 - 1. 536 mg/L. The good extraction recoveries were obtained for the spiked samples （84.7%, 89.3% and 87.7% for low, middle and high concentrations of added standards, respectively）. The relative standard deviation of intra-day and inter-day precisions was in the range from 0.6% to 13.5%. The pharmacokinetic study of 4-O-methylhonokiol was made and the results from the plasmaconcentration curve of 4-0-methylhonokiol showed a two-apartment open model. This work developed a sensitive, stable and rapid HPLC method for the determination of 4-O-methylhonokiol and the developed method has been successfully applied to a pharmacokinetic study of 4-O-methylhonokiol.

Objective: To investigate the relationships between the expression of insulin-like growth factor-1 (IGF-1) in the gastric cancer tissue and the clinicopathological parameters in the gastric cancer patients in China with Meta-analysis,and to provide the evidence-based medical evidence for the diagnosis and treatment of gastric cancer. Methods: The literatures about the relationships between the positive expression rate of IGF-1 and the clinicopathological paramters in the gastric cancer patients were searched comprehensively in PubMed, China National Knowledge Infrastructure, Weipu Chinese Science and Technology Journal Database,Wanfang Database and China Biomedical Literature Database. Review Manager 5. 2 software was used to conduct Meta analysis on the literatures that met the inclusion criteria. Results: There were 11 articles in the literatures were selected∗ including 993 cases in gastric cancer group and 601 cases in non-tumor gastric mucosal tissue control group. Meta-analysis showed the significant differences in the positive expression rates of IGF-1 between gastric cancer group and control group (OR = 9. 05,95% CI: 6.82 12.01), poorly differentiated gastric cancer group and moderately-highly differentiated group (OR=l. 53 95% CI: 1.14-2.06). involving full-thickness group and unaffected full- Thickness group (OR = 0. 16. 95% CI: 0. 09 - 0. 28). lymph node metastasis group and non-lymph node metastasis group (GR = 0. 12, 95% CI: 0.08 0.17), TNM stage 111 - IV group and TNM stage 1-1! group (OR = 0.21, 95% CI: 0. 13 0. 33). Conclusion: The high expression of IGF-1 in gastric cancer tissue is closely related to the occurrence, development, invasion and metastasis of tumor in the gastric cancer patients in China. The abnormal expression of IGF-1 could be used as a risk factor for carcinogenesis of gastric mucosal cells and a valuable molecular biological marker for early diagnosis and treatment of gastric cancer.

Objective To observe the effect of low concentration Aβ1-42 monomer/oligomers and CORM-2 in different concentration on livability of SN56 cells. Methods SN56 cells were cultured in the 96-well plate with uniform concentration, and were divided into control group, Aβ1-42 group, Aβ1-42 + CORM-2 50μM group, and Aβ1-42 + CORM-2 100 μM group. Three lines of cells in Aβ1-42 group were cultured in the surroundings of 10nM,100nM and 1 μM Aβ1-42monomer/oligomers, respectively. Aβ1-42 + CORM-2 50μM group and Aβ1-42 + CORM-2 100μM group had the same culture condition as group Aβ1-42 ,except contain 50μM, and 100μM CORM-2, respectively. Control group didnt have any effect factor. Three days later,the livability of different groups was compared with MTT method. Results The livability of group Aβ1-42 with the increasing concentration of Aβ1-42 was (79.73 ±0.94)% ,(67.99 ±0.79)% ,(60.42 ±0.62)% , respectively. The higher the concentration of Aβ1-42 was,the lower the livability of SN56 cell was. The livability of group Aβ1-42 + CORM-2 50μM/100μM was( 75.15±0.096)%,(63.20 ±0.17)%, (55.33 ±0.19)%; (73.20 ±0.27)%, (64.34 ±0.11 )%, (54.17 ±0.12)% , respectively. Both were lower than group Aβ1-42. And different CORM-2 concentration had discrepancy in the ability of decreasing the cell livability. Conclusion Low concentration of Aβ1-42 can reduce the livability of SN56 cells, and higher concentration has more significant effect; CORM-2 in different concentration both can decrease the livability of SN56 cells,and there is a discrepancy in the intensity.

The aim of this study is to construct specific shRNA expressing plasmids, and to observe their effects on H9c2 cardiomyocytes injury induced by hypoxia/reoxygenation (H/R). RIPK1 and RIPK3 are the key kinases mediating the process of necroptosis. Using recombinant DNA technology, we inserted the synthetic shRNA into pSUPER vector to construct RIPK1-shRNA or RIPK3-shRNA plasmid respectively. We transfected H9c2 cardiomyocytes with the two shRNA plasmids respectively, before we treated them with H/R stimulation. Then, we measured the relevant genes and proteins by real-time PCR and Western blot. Meanwhile,we detected the markers of necroptosis and cardiomyocytes injury. The results showed that inhibition of ripk1 or ripk3 gene expression by its specific shRNA might protect the cardiomyocytes injury induced by H/R stimulation.
Animals ; Apoptosis ; Cell Hypoxia ; Cell Line ; Gene Expression ; Myocytes, Cardiac ; pathology ; Protein-Serine-Threonine Kinases ; genetics ; metabolism ; RNA, Small Interfering ; Rats ; Real-Time Polymerase Chain Reaction ; Receptor-Interacting Protein Serine-Threonine Kinases ; genetics ; metabolism ; Transfection