Twelve specimens of genital warts were excised from 12 patients and divided into three parts. One part was untreated, the second and the third part were treated with CO_2 laser and microwave respectively. HPV DNA was amplified and detected in 100% of untreated specimens (HPV16 and HPV11 in six patients each), in 83% and 50% of specimens treated with CO_2 laser and microwave respectively. There was a significant difference in the detection rates between untreated and microwave treated specimens (X~2=4.18, P

砄bjective:To observe streptococcus sanguis (S.s) and Actinomycetes viscosus (A.v) in oral plaque biofilm formation.Methods:20 ml of saliva obtained from a health adult was centrifuged at 4 ℃ and 10 000 r/min for 10 min.The supernatant was disinfected in 60 ℃ water bath for 30 min.Glass coverslips in the size of 24 mm?24 mm were immersed into the saliva supernatant for 2 h to obtain biofilm.100 ?l of S.s ATCC 34 and A.v ATCC 19246 mixture cultured in TSB at the density of 10 5～6 CFU/ml was added into 20 ml of TSB,and then,the coverslips with biofilm were put into the mixture.The biofilm and bacteria were observed by scanning confocal laser microscopy at various times.Resuts:The biofilm reached the thickness of 15.4 ?m in 8 h and the clumps of the bacteria were mostly in the midle layer of the biofilm.The biofilm increased to 34.3 ?m in 16 h and became tassle like in 48 h.Conclusion: S.s and A.v may play some roles in the oral biofilm formation.

Objective: To develop a new antimicrobial sensitivity test model for oral products in vitro. Methods: An artificial biofilm mouth model for antimicrobial sensitivity test was established by modified LKB chromatography chamber. Using sodium fluoride and tea polyphenol as antimicrobial agents and Streptococcus mutans as target, various methodologies on the sensitivity tests were compared. Results: Agar diffusion, agar dilution and broth macro-dilution were more sensitive than modeling biofilms method. The modeling biofilm assay resulted in MIC values of fluoride or tea polyphenol against S. mutans 32 and 12 times higher respectively than the MIC values generated by broth macro-dilution method. Conclusion :The biofilm artificial mouth model may simulate the environment for oral products test.

Objective To explore the mechanism of the reversibility of chronic cyclosporine (CsA) nephrotoxicity in rats after long-term drug withdrawal. Methods Chronic CsA nephrotoxicity was induced in Sprague-Dawley rats by administering CsA(15 mg . kg-1. d-1) for 5 weeks,and then the effect of drug withdraw for 5 and 10 weeks was observed. Body weight,systolic blood pressure,and renal function were monitored. In addition,renal histopathology(arteriolopathy,ED-1-positive cells,and tubulointerstitial fibrosis) and expression of osteopontin(OPN) and transforming growth factor(TGF) -?1 mRNA was examined. Results Compared with the control rats,CsA-treated rats showed loss of body weight,deterioration in renal function and development of typical histopathology. All of these above parameters were significantly reversed,meanwhile,the upregulation of OPN and TGF-?1 mRNA expression decreased significantly at 5th and 10th week after CsA withdrawal. Of note,the decreased OPN and TGF-?1 mRNA expression was positively correlated with the reduction in the tubulointerstitial fibrosis score. Conclusion The established chronic CsA nephrotoxicity is reversible after long-term CsA discontinuation,and the mechanism may be associated with the down-regulation of OPN and TGF-?1 mRNA expression.

OBJECTIVE:To establish a method of the uncertainty evaluation of the potency testing of Oxytocin injection. METHODS:Mathematical model was established for potency testing of Oxytocin injection to analyze the influential factors of un-certainty. The uncertainty components were quantitatively analyzed and the uncertainty was calculated. RESULTS:The expanded un-certainty of the potency testing of Oxytocin injection was 0.20 u/ml and the potency was(9.29±0.20)u/ml,coverage factor K=2. CONCLUSIONS:The method is suitable for the uncertainty evaluation of potency testing of Oxytocin injection.

Objective Analysis the clinical biobanks sustainable development and influence,and give some advices for the development of clinical biobanks.Methods By review the development of Beijing Biobank of Clinical Resources,and combined with the related literature,we analysis the clinical biobanks sustainable development and influence.Results With the rapid development of the biobank,its sustainable development has attracted more and more attention,and is influenced by sharing,funds,staff,information,strategies and policies.Conclusions The sustainable development of clinical biobanks is facing more and more challenges.For the sustainable development of clinical biobanks,the constructer should be focus on the requirement from the research,and pay attention to the interests of stakeholders.

Objective To investigate the inhibitory effects of traditional Chinese medicine (TCM) on hypermelanosis and provide experimental evidence for treating skin pigmentary disorders. Methods Five TCMs with strong inhibitory effects on tyrosinase activity were tested. The changes of the number and morphology of melanocytes induced by UVB were observed in the experimental hypermelanosis model (brownish guinea pig). Results Decreased melanocytes and melanin granules were found with the treatment of Poria cocos, Polyporus umbellatus, Cornus officinalis Sieb. et Zucc., Atractylodes macrocephala Koidz., Astoagalus complanatus R. Br. ex Bge. Conclusion There are inhibitory effects on hypermelanosis induced by UVB with the treatment of Poria cocs, Polyporus umbellatus, Cornus officinalis Sieb.et Zucc., Atractylodes macrocephala Koidz., Astoagalus complanatus R. Br.ex Bge.

Objective To observe the effects of negative pressure wound therapy (NPWT) on survival of the blade-thickness free skin grafts. Methods Sixty-five patients with skin defects were divided into NPWT treatment group ( Group Ⅰ,n =35) and conventional treatment group ( Group Ⅱ,n =30) according to different postoperative fixation methods.The patients in Group Ⅰ were fixed with the aid of NPWT after blade-thickness free skin grafting,and the patients in Group Ⅱ were fixed with tie-over bolster dressing. Results The survival rate of the skin grafts of Group Ⅰ was significantly higher than that of Group Ⅱ at day5 postoperatively [ (80.59 ±10.30)％ vs (71.46 ±10.68)％,P＜0.05].The survival period for the skin grafts of Group Ⅰ was shorter than that of Group Ⅱ[ (5.34 ± 0.87) days vs ( 11.20 ± 1.65) days,P ＜ 0.01].The duration of postoperative hospital stay of Group Ⅰ was obviously shorter than that of Group Ⅱ (P ＜ 0.01 ).The cost of antibiotics of Group Ⅰ was less than that of Group Ⅱ [ ( 1 765.71 ± 164.39) RMB yuan vs (2 700.00 ±221.28) RMB yuan,P ＜0.01].The dressing frequency and cost of group Ⅰ were less than those of group Ⅱ [(3.11 ± 0.32) times,(249.14 ±25.82) RMB yuan vs (4.53 ±0.68) times,(362.67 ±54.52) RMB yuan,P＜0.01]. Conclusions The application of NPWT to the postoperative period of skin grafting can promote the survival of the skin grafts,shorten the duration of hospitalization and reduce antibiotics use and dressing frequency.

Acupuncture for stroke rehabilitation is of multi-targets, the treatments of which are not only for hemiplegia, but also cover a wide variety of aspects, such as aphasia, dysphagia, dysarthria, cognitive disorders, bowel or bladder dysfunction, ataxia and post hemiple-gic pain, etc. Based on the experiences of stroke rehabilitation with Integrated Traditional Chinese and Western Medicine over 20 years, this paper discussed the selection of acupoints, needling manipulations, opportunity of intervention, quantity of stimulations, the needle-retaining time as well as the impact on spasticity after hemiplegia referring to ancient and modern literatures and modern rehabilitation theory.

OBJECTIVE:To optimize the inclusion process of the volatile oil of Pogostemon cablin and Acorus tatarinowii in Xiaoyu anshen capsule.METHODS:The inclusion process was carried out by saturated water solution method.And the inclusion process was optimized by orthogonal experiment with the inclusion ratio and yield of the volatile oil as indexes taking the ratio of volatile oil to ?-CD,the temperature and the time of inclusion as factors.The physical phase of the inclusion complex was detected by differential scanning calorimetry(DSC).RESULTS:The optimum inclusion process was established as follows:the ratio of oil to ?-CD=1∶8;the ratio of water to ?-CD=10∶1;the inclusion time was 1h and the inclusion temperature was 50 ℃.The inclusion rate was as high as 90%.The DSC revealed that a new physical phase has been formed for the inclusion complex.CONCLUSION:The intermolecular inclusion complex with good stability and high yield of volatile oil could be obtained from the optimized inclusion process.