OBJECTIVEThe non-specific antitumor immunity effect of 4-1BBL-B7-H3 gene was investigated by establishing an oral squamous cell carcinoma human peripheral blood lymphocyte-severe combined immunodeficient (SCID) mice chimeric model.

METHODSForty mice were randomly divided into five groups. All groups, except the non-immune reconstitution group (group D), had reconstructed human partial immune system. The control group (group A) was injected with Tca8113 cells. The Ad4-1BBL-B7-H3 group (group B) was injected with Tca8113 cells transfected by adenovirus containing 4-1BBL-B7-H3 gene. The empty vector group (group C) was injected with Tca8113 cells transfected by adenovirus containing an empty vector. The non-immune reconstitution group (group D) was injected with Tca8113 cells. The non-tumor group (group E) was injected with PBS. The tumor volumes in each group were measured weekly. Human IgG in blood was obtained through the tail vein and was determined by enzyme-linked immunosorbent assay. Human CD3+ and D56 lymphocytes were assessed by flow cytometry. Model animals were killed on the ninth week. Differences in the expression of the natural killer group 2 member D (NKG2D) and toll-like receptor 2 (TLR2) in tumor tissues of each group were observed by immunohistochemical method. 4-1BBL-B7-H3 gene expression in mice tumor tissues was detected by reverse transcription polymerase chain reaction (PCR) and the expressions of major histocompatibility complex 1 class related molecule (M1C) A, M1CB, and TLR2 were detected by real-time quantitative PCR.

RESULTSThe tumor volumes of group B were remarkably lower than those in the other groups (P < 0.05). Human IgG and CD3+ and CD56+ lymphocytes were detected in the peripheral blood of immune-reconstituted mice. These lymphocytes were remarkably higher in group B than those in groups A, C, and E (P < 0.05). Higher NKG2D and TLR2 expression were observed in group B tumor than those in the other groups. The stable expression of 4-1BBL-B7-H3 gene in group B was proven. The expression of M1CA, M1CB, and TLR2 were significantly higher in the group B tumor than those in groups A, C, and D (P < 0.05).

CONCLUSIONThe high 4-1BBL-B7-H3 gene expression in tumor tissues could successfully induce the proliferation of CD3+ and CD56+ lymphocytes. This expression can also directly or indirectly activate TLR2 and up-regulate the expression of NKG2D and its ligands (M1CA and M1CB), which result in an effective antitumor immune response.

4-1BB Ligand ; Adenoviridae ; Animals ; Carcinoma, Squamous Cell ; Disease Models, Animal ; Genetic Vectors ; Humans ; Mice ; Mice, SCID ; Mouth Neoplasms ; Neoplasms ; Transfection

Objective To establish a quality indicator system of nursing services for patients with urodynamics. Methods Delphi method was used in the study, and the quality indicator system of nursing services for patients was based on two rounds of consultation among 16 experts. Results The quality indicator system of nursing services for patients with urodynamics consisted of 3 first-level indicators, 8 second-level indicators and 29 third-level indicators. Experts had the same opinions on the indicators. Conclusions Nursing behavior for patients with urodynamics could be standardized based on the quality indicator system, and therefore guide nursing work and improve nursing quality. Further theoretical and empirical study is needed to verify the quality indicator system.

Objective:To explore the effects of NOD2 gene on the proliferation and apoptosis of tongue squamous cell carcinoma Tca8113 cells.Methods:NOD2 expression vector(NOD2-pEZ-M29)and NOD2-shRNA vector were established,then were trans-fected into Tca8113 cells respectively.Expressions of HBD-2 and NOD2 in the cells was detected by RT-PCR and Western blot.Cell proliferation was examined by MTT assay and apoptosis by flow cytometry at 48h post transfection.Results:Compared with the control group,the expression of NOD2 and HBD-2 in NOD2-pEZ-M29 transfection group was significantly higher and markedly lower in NOD2-shRNA group.The proliferation rate of Tca8113 cells was markedly lower in NOD2-pEZ-M29 transfection group and signifi-cantly higher in NOD2-shRNA group while the apoptosis rate was significantly higher in NOD2-pEZ-M29 transfection group and sig-nificantly lower in NOD2-shRNA group.Conclusion:In Tca8113 cells NOD2 expression was positively correlated with HBD-2 ex-pression.NOD2 gene may promote the apoptosis,inhibit the proliferation of Tca8113 cells.

A new method was established for the direct, rapid and quantitative analysis of pesticide residues, dimethoate chlorothalonil and malathion by low temperature plasma ( LTP) ionization miniature ion trap mass spectrometer. The LTP ionization probe and sample inlet of ion trap mass spectrometry were enclosed in a metal cavity. With non-contact heating, the samples placed on the sample platform were desorbed into gaseous phase and ionized by LTP ionization probe. The results showed that closed ionization had an edge over the opened ionization. The quantitative analysis of 3 pesticides within the range of 0. 5-10 mg/L was realized by optimizing heating time and flow rate of air, and the relative standard deviations of signal intensity is less than 11%. LODs of pesticide, which were obtained within 5 s, were as low as several hundred pictograms. The results showed that the method could be used for the analysis of pesticide residue on green and organic fruits or vegetables.

By reviewing the concept, content, evaluation tools and significance to clinical practice of preoperative patients' preparedness for selective surgery, this paper aims to provide some reference for enriching the content of preoperative patients' preparedness and improving the surgical satisfaction and quality of life for selective surgery.

Objective:To explore the application effect of non-immersive virtual reality technology in preoperative visit of gynecological robotic surgery patients, and to provide reference for improving the preoperative visit effect in the operating room.Methods:By adopting a quasi-experimental study, a total of 80 patients who underwent Da Vinci robot-assisted gynecological surgery in the First Affiliated Hospital of Zhengzhou University were selected. The eligible patients from January to March 2022 were selected as the control group (39 cases), and the patients from April to June 2022 were selected as the observation group (41 cases). The control group used routine preoperative visit sheet and education video for preoperative visit, while the observation group used preoperative visit sheet combined with virtual reality technology for preoperative visit. The anxiety and tension, the degree of coordination of body position and the satisfaction of preoperative visit were compared between the two groups.Results:After intervention, the anxiety score, blood pressure and heart rate of the observation group patients were (38.12 ± 2.47) points, (76.37 ± 7.53) mmHg(1 mmHg=0.133 kPa), (119.20 ± 5.88) mmHg, and (79.51 ± 8.38) times/minute, respectively, which were significantly lower than the control group′s (44.56 ± 5.10) points, (82.77 ± 8.97) mmHg, (125.74 ± 8.21) mmHg, and (84.92 ± 7.48) times/min, and the differences were statistically significant ( t values were 3.04-7.25, all P<0.05). The proportion of patients in the observation group who did not require assistance and needed partial assistance and needed the full assistance of itinerant nurses in position placement were 51.22% (21/41), 41.46% (17/41), 7.32% (3/41), while those in the control group were 23.08% (9/39), 56.41% (22/39), 20.51% (8/39), and the difference was statistically significant ( Z=2.74, P<0.05). The satisfaction score of preoperative visit in the observation group was (2.66 ± 0.48) points, which was higher than (2.18 ± 0.56) points in the control group, and the difference was statistically significant ( t=-4.12, P<0.05). Conclusions:Non-immersive virtual reality technology makes the preoperative interview of Da Vinci robotic surgery more vivid, which can alleviate the preoperative anxiety of patients, improve the autonomy of patients' position placement and the satisfaction of patients with the preoperative interview.

Objective To establish a scientific and reasonable evaluation index system of nursing quality for cystoscopy. Methods After 2 rounds of Delphi survey technique, get the consensus of the evaluation indicators of the initial evaluation of the 18 experts,and ultimately build a system for nursing quality evaluation index during cystoscopy. Results After 2 rounds of expert consultation, the evaluation index system based on the three level structure of the medical quality framework includes:3 first-level indicators,8 second-level indicators,33 third-level indicators. Conclusions The evaluation index system established in this study is helpful to standardize the nursing behaviors of nurses in the examination process,improve the quality of nursing service in the process of cystoscopy, and improve the accuracy and reliability of inspection.

OBJECTIVE To investigate the mechanism of Yifei xuanfei jiangzhuo formula （YFXF） against vascular dementia （VD）. METHODS The differentially expressed genes of YFXF （YDEGs） were obtained by network pharmacology. High-risk genes were screened from YDEGs by using the nomogram model. The optimal machine learning models in generalized linear， support vector machine， extreme gradient boosting and random forest models were screened based on high-risk genes. VD model rats were established by bilateral common carotid artery occlusion， and were randomly divided into model group and YFXF group （12.18 g/kg， by the total amount of crude drugs）， and sham operation group was established additionally， with 6 rats in each group. The effects of YFXF on behavior （using escape latency and times of crossing platform as indexes）， histopathologic changes of cerebral cortex， and the expression of proteins related to the secreted phosphoprotein 1 （SPP1）/phosphoinositide 3-kinase （PI3K）/protein kinase B （aka Akt） signaling pathway and the mRNA expression of SPP1 in cerebral cortex of VD rats were evaluated. RESULTS A total of 6 YDEGs were obtained， among which SPP1， CCL2， HMOX1 and HSPB1 may be high-risk genes of VD. The generalized linear model based on high-risk genes had the highest prediction accuracy （area under the curve of 0.954）. Compared with the model group， YFXF could significantly shorten the escape latency of VD rats， significantly increase the times of crossing platform （P＜0.05）； improve the pathological damage of cerebral cortex， such as neuronal shrinkage and neuronal necrosis； significantly reduce the expressions of SPP1 protein and mRNA （P＜0.05）， while significantly increase the phosphorylation levels of PI3K and Akt （P＜0.05）. CONCLUSIONS VD high-risk genes SPP1， CCL2， HMOX1 and HSPB1 may be the important targets of YFXF. YFXF may play an anti-VD role by down-regulating the protein and mRNA expressions of SPP1 and activating PI3K/Akt signaling pathway.