BACKGROUND: Revascularization of trachea following trachea transplantation needs to be solved.OBJECTIVE: To explore the empirical methods of allogeneil graft of long-segment trachea and its revascularization.DESIGN, TIME AND SETTING: The animal observation experiment was performed at the Department of Chest Surgery, Shandong Provincial Chest Hospital between June 2007 and June 2008.MATERIALS: Totally 20 healthy, New Zealand rabbits, were provided by animal center of Medical School of Shandong University. Additional 10 rabbits were used as donors, and 10 rabbits were served as recipients.METHODS: The mucosa and smooth muscle in trachea of donor rabbits was removed, and the anular ligaments were shear opened or intensive drilling to obtain tracheal cartilage scaffold with fissure or mesh. A jejunum with vascular pedicle was harvested from recipient rabbits, which was longer than tracheal cartilage scaffold. The cartilages rings were wrapped with greater omentum. Finally, the constructed simulating trachea was replaced in the abdominal cavity. MAIN OUTCOME MEASURES: Growth of retina and tracheal cartilage.RESULTS: Abdominal cavity of recipient rabbit was opened after 2 weeks, and it was observed with gross observation and pathological section: There was no collapse in the lumens of tracheal allografts with good elasticity tracheal wall. The blood of omentum and intestinalmucosa that wrapped tracheal allograft was circulating well; and there was no cellular necrosis and merging in xenogenic cartilagines tracheales. CONCLUSION: The study fulfilled the stage one reconstruction and revascularization of tracheal allograft in abdominal cavity of recipient. Stenopeic tracheal stand wrapped with pedicled omentum and intestinalmucosa of recipient made allograft not restricted by length, which is critical to revascularization of long-segment trachea.

Objective To explore the therapeutic efficacy and adverse effects of decitabine combined with half dose CAG regimen and only CAG regimen for the elderly patients with acute myeloid leukemia (AML).Methods A total of 42 elderly patients with AML aged between 65 and 75 years old (except for acute promyelocytic leukemia) admitted into Heze Municipal Hospital from August 2013 to August 2017 were retrospectively analyzed.They were divided into treatment group and control group according to the different chemotherapy regimens.Twenty patients in the treatment group were treated with decitabine combined with half dose CAG regimen (recombinant granulocyte colony stimulating factor + cytarabine + aclarubicin),and 22 patients in the control group were treated with CAG regimen.Results After the first course of treatment,in treatment group,13 cases achieved complete remission (CR),3 cases achieved partial remission (PR),4 cases achieved non-remission (NR),and the total efficacy (CR+PR) rate was 80.0 ％ (16/20).In control group,8 cases (36 ％) achieved CR,2 cases achieved PR,12 cases achieved NR,and the total efficacy rate was 45.5 ％ (10/22).The difference in total effective rate between the two groups was statistically significant (x2 =3.707,P =0.035).There was no significant difference in the bone marrow recovery time,the infusion of red blood cells and platelets between the two groups (all P ＞ 0.05).Conclusion The therapeutic efficacy of decitabine combined with half dose CAG regimen is better than that of CAG regimen,the adverse effects are all tolerated,and it can be served as the prior therapy for elderly AML patients.