273 spine apparatus were observed under electron microscope. The results were as follows: 1.The flat-type sac of 5 types of the sac which constituted the spine apparatus covered the majority. Most of them were arranged parallelly and a few dispersively. The horseshoe or braid shaped arrangement was also seen in some cases. 2.The number of the sac varied from 3 to 6 in most of the cases, and a few over 10. Sometimes 2 or 3 spine apparatus can be seen within a single dendritic spine. 3.The spine apparatus often locates in the dendritic spine. In some cases the spine apparatus can be seen within the dendrites or in the juncture of dendritic trunk and dendritic spine 4.The morphological structure of the dense bands is comparatively complex. Most of the bends are narrow, some are broad, fine granular or mist-like in shape. The dense bends are located in between the adjacent sacs mostly and in few cases on one side of the spine apparatus. In some cases, one end of the dense band is fused with the sac.

The distribution and morphology of supraependymal cells residing within thethird ventricle of the adult rabbit and adult rat have been investigated by means ofscanning electron microscopy.Supraependymal cells can be classified into twofundamental types,those with neuronal characteristics were designated Type Ⅰ incontrast to those that appear to be macrophagic in character,which were termedType Ⅱ.The neuron-like supraependymal cells have oval cell bodies and long slen-der processes.They occurred not only singly along the ventricular surface,but alsotended to occur in clusters which may be named as neuronal complex.Usually theneuronlike structures possessed varicose fibers of various calibers,runing among thecilia,microvilli,and bulbous protrusions of the ependymal surface.The morphologyof macrophage-like ependymal cells are variable.In general,they had one or severalbroad pseudopodia.According to the number of processes,the macrophage-likesupraependymal cells may be divided into three main types.The morphology of supraependymal cells within the third ventricle of the rabbitand rat is quite similar.The role of supraependymal cells were discussed.

Objective To investigate the clinical significance and effect of total hip arthroplasty (THA) in treatment of bony ankylosis of the hip in patients with ankylosing spondylitis (AS).Methods The study enrolled 23 patients (35 hips) undergone THA for bony ankylosis of the hip in AS between March 2008 and November 2012.Postoperative outcome was measured based on Harris hip score and Xray images.Results Preoperative Harris hip score of (27.6 ± 2.3) points increased to (86.7 ± 2.6)points postoperatively.Hip flexion and adduction deformity presented correction postoperatively.Total hip range of motion (flexion,adduction,abduction,and external rotation) improved from (45.7 ±5.3) o preoperatively to (206.9-±4.7) °postoperatively and mean hip flexion activity was 81 °.Hip activity achieved significant improvement and pain was relieved.X-ray films revealed proper position of prostheses without the presence of loosening or subsidence.Two patients (3 hips) experienced mild pain after a long period of walking.Four patients had heterotypic ossification including Brooker grade Ⅱ in one patient and Brooker grade Ⅰ in three.Conclusion THA is an effective treatment for bony ankylosis of the hip in AS,for it rebuilds joint function,relieves pain of the hip and improves patients' quality of life.

The choroid plexus of the cat, rabbit and rat have been investigated by means of scanning elecron microscopy. The surface appearance of choroid plexus of these animals exhibited pleomorphism. The free surface of epithelial cells are covered with dense microvilli, single long cilium, clusters of cilia, bulbous protrusions and flower-like structure. They are scattered throughout the ventricular surface of the choroid plexus. High magnification scanning electron microscopy reveals that the population of microvilli consist of slender finger-like microvilli and microvilli with the bleb-like protrusion. Flower-like structure is the clusters of microvilli. On the surface of the choroid plexus of these animals, the Kolmer cells were observed. According to the number of processes, the Kolmer cells of cat, rabbit and rat may be divided into four main types. The ultra-architectural organization of choroid plexus of the cat, rabbit and rat is quite similar, but the number of bulbous protrusions in the cat and clusters of cilia in the rat occurs more.

Objective:To investigate the protective effects of quercetin on the periodontal tissue in Wistar rats with periodontitis. Methods:30 Wistar rats were randomly divided into 3 groups(n =1 0):Control group (group N),ligation group(group L)and liga-tion +quercetin group(group LQ).Bilateral first molars of the rats in group L and LQ were cervically ligated by an orthodontic wire with the diameter of 0.2 mm.The rats in group LQ were then treated with quercetin by gavage at 50 mg/(kg·d)after ligation.After 8 weeks,the rats were killed.Continuous slices of the periodontal tissue were made for HE staining and immunohistochemistry stai-ning.The morphological changes and the expression of TNF-αand COX-2 in the periodontal tissue were observed under light micro-scope.Results:The inflammatory cells and periodontal pocket were observed in group LQ were less than those in group L.The levels of TNF-α,COX-2 in group LQ were lower than that those in the group L(P <0.05).Conclusion:Quercetin has a protective effect on the periodontal tissues of Wistar rats with periodontitis.

Objective To investigate the therapeutic effect of autologous stem cell transplantation research for patients with cerebral hemorrhage sequelae under the stereotactic.Methods One hundred patients with cerebral hemorrhage from Jan.2011 to Sep.2013 in our hospital were selected and randomly divided into the experimental group (n =50) and the control group (n =50).The patients of experimental group were given autologous stem cell transplantation under the stereotactic in 6 months after cerebral hemorrhage,while the patients in control group were just given traditional treatment.At 6,7 and 12 months after cerebral hemorrhage,rate with neural function defect scale and functional independence measure(FIM) scores of the two groups were compared.Results FIM scores in the experimental group was 102.08 ± 8.28,significant higher than that in control group(95.28±8.75,P＜0.05).Functional independence measure scores in the experimental group at 7 months after cerebral hemorrhage was 13.12±4.00,significant lower than that in control group(20.40±4.33,P ＜0.05).While,there was no statistical difference at 6 months and 12 months after cerebral hemorrhage between the two groups(P＞0.05).Conclusion The therapeutic of autologous stem cell transplantation on patients with cerebral hemorrhage sequelae under the stereotactic is benefit at short term,but the long term therapy effective still needs further study.

Objective To compare the whole-body equivalent doses from volumetric modulated arc therapy (VMAT) and static intensity-modulated radiotherapy (IMRT) for patients with cervical cancer.Methods Nine patients with cervical cancer admitted to our hospital in 2014 were included in this study.Both VMAT and IMRT were planned for each patient.Each patient's personal dose equivalent (Hp (10)) was measured using thermoluminescent dosimeters placed at the xiphoid process and glabella during IMRT and VMAT.The whole-body equivalent doses were estimated based on the results measured at the xiphoid process and compared between the VMAT and IMRT techniques.The paired t test was used for difference analysis.Results The Hp (10) values measured at the xiphoid process and glabella of every patient were lower for VMAT than for IMRT.At a prescribed dose of 50 Gy,if the mean Hp (10) values measured at the xiphoid process were considered to represent the whole-body equivalent doses,the whole-body equivalent doses for VMAT and IMRT were 364 mSv and 538 mSv,respectively.Conclusions VMAT results in a lower whole-body equivalent dose to patients compared with IMRT.The decreased whole-body equivalent dose delivered by VMAT may reduce the likelihood of a radiation-induced secondary malignancy.

Objective To investigate the radiographic classification and presentation of atypical spinal tuberculosis in adults.Methods Data of 45 consecutive patients who had suffered from atypical spinal tuberculosis confirmed by surgical interventions and pathologic examinations at our department from February 2000 to August 2012 were retrospectively analyzed.There were 29 males and 16 females,aged from 20 to 71 years (mean,46.2 years).Twenty-five patients presented with low-grade fever,lassitude and loss of weight,and the other 20 patients denied the constitutional symptoms of tuberculosis.An increased erythrocyte sedimentation rate (range,25-107 mm/1 h) was found in 37 patients.All patients were investigated by the following imaging resources:plain X-rays,CT scan and MRI.3D reconstruction CT was performed in 12 patients and PET-CT was performed in 2 patients.All patients were treated surgically and confirmed by pathologic examinations and 3 patients had undergone CT-guided biopsy.Results Atypical spinal tuberculosis presented in different radiographic presentation forms.Nine patients had involvement of a single vertebral body,which was depicted with nonuniformly increased signal intensity on T2-weighted MR images.CT scan showed irregular bony destruction in old patients and solitary osteolytic lesion with well-defined margins in young adults.There were 2 cases of isolated affection in the posterior elements (vertebral appendages type),and the involved vertebral appendages demonstrated hyperintense signal on T2-weighted MR images and bony destruction on CT scan.There were 8 cases of simultaneous affection in the anterior and posterior element of one vertebra (circumferential vertebral involvement type),and CT scan showed multiple bony destruction in vertebral body and neural arch.There were 5 cases of affection in disc (intervertebral disc involvement type),which presented decreased signal in MRI and protruding disc pressing the dural sac could be found.There were 14 cases of multiple vertebral tuberculosis in continuity (contiguous spinal tuberculosis),which presented multiple tuberculosis lesions on 3D-CT.There were 7 cases of multiple vertebral tuberculosis in noncontinuity (noncontiguous spinal tuberculosis),which presented destructive tubercular lesions affected different levels in different regions of the spine on MRI.Conclusion Atypical spinal tuberculosis had multiply radiographic presentation forms and atypical radiographic features.Worm-eaten destruction of bone or vertebral endplate,marrow edema and increased signal intensity of paraspinal ligament are features of radiographic presentation in diagnosis of atypical spinal tuberculosis.

In order to find out a parameter for the medical diagnosis and therapeutic criteria of endemic fluorosis, the excretory level of fluorine in urine was determined between the patients of bone fluorosclerosis and those who lived in the district of endemic fluorosis. The results showed that both the patients and the normal people in the endemic area of fluorosis excreted more fluorine in urine than the others, because of drinking highly fluorina-ted water. Thus a positive correlation was found between the amount of fluorine in urine and the degree of fluorinated drinking water, while no significant difference was found between the patients and the normal individuals. The urinary output of fluorine fluctuated in a few cases of fluorosclerosis.From the data obtained we may, perhaps, conclude that the excretion rate of fluorine in urine may be considered as a parameter of fluorine overload in the body, but not as applicable to the medical diagnosis or treatment in fluorosis.

Objective To discussion the feasibility and effect of total hip arthroplasty (THA) of postoperative ankylosis in patients with hip peripheral fracture.Methods From January 2008 to October2013,cementless THA was performed in 23 patients with ankylosis after internal fixation of hip peripheral fracture.There were 16 males and 7 females,aged 23 to 67 years (mean,43 years).Interval between internal fixation and THA was 12 to 73 months (mean,38 months).Results Mean follow-up was 28 months (range,3 to 60 months).All patients presented good press-fit prostheses with mean acetabular valgus of 43.3°,mean acetabular anteversion of 22.5°,and mean femoral anteversion of 16.4°.Primary wound healing was detected with no occurrence of infection,prosthetic loosening or dislocation,and periprosthetic fracture.Femoral vein incomplete embolism was diagnosed in one patient with limb swelling 2 months after operation,but swelling subsided after 3 months of bed rest,elevation of the affected limb and anticoagulant therapy.Length of the bilateral limbs was almost equal in 19 patients with the difference within 0.5 cm,but the affected limb was 0.8-1.4 cm shorter in 3 patients and was 0.8 cm longer in 1 patient.Harris hip score improved from (42.16 ± 3.03)points before operation to (93.08 ± 5.23) points at the last follow-up (P ＜ 0.05).Conclusion THA is associated with good hip function recovery and improved quality of life during the treatment of postoperative ankylosis in patients with hip peripheral fracture.