Objective: To study the anticaries effects of salivary gland extract (SGE). Methods: Dental caries model was established in 80 SD rats by feeding with cariogenic diet 2000 and by infecting with cariogenic bacteria. 20 model rats in each of the 4 experimental group were fed with SGE at 400 mg/kg, 1 500 mg/kg or 5 mg/L of NaF or deionic water respectively,another 20 were fed with ordinary diet as blank control. The anticaries effects were evaluated by Keyes caries scores, SOD activity in blood cells was determined by NBT test. The effect of SGE on dissoluble Ca 2+ from teeth was determined in electrochemical artificial caries model in vitro. Results: caries was not observed in the blank control. According to Keyes caries score, at E, Ds, Dm and Dx levels, the score in SGE groups was significantly lower than that in the model group (P0.05). SGE obviously enhanced SOD activity (P

Objective To investigate the therapeutic effect of autologous stem cell transplantation research for patients with cerebral hemorrhage sequelae under the stereotactic.Methods One hundred patients with cerebral hemorrhage from Jan.2011 to Sep.2013 in our hospital were selected and randomly divided into the experimental group (n =50) and the control group (n =50).The patients of experimental group were given autologous stem cell transplantation under the stereotactic in 6 months after cerebral hemorrhage,while the patients in control group were just given traditional treatment.At 6,7 and 12 months after cerebral hemorrhage,rate with neural function defect scale and functional independence measure(FIM) scores of the two groups were compared.Results FIM scores in the experimental group was 102.08 ± 8.28,significant higher than that in control group(95.28±8.75,P＜0.05).Functional independence measure scores in the experimental group at 7 months after cerebral hemorrhage was 13.12±4.00,significant lower than that in control group(20.40±4.33,P ＜0.05).While,there was no statistical difference at 6 months and 12 months after cerebral hemorrhage between the two groups(P＞0.05).Conclusion The therapeutic of autologous stem cell transplantation on patients with cerebral hemorrhage sequelae under the stereotactic is benefit at short term,but the long term therapy effective still needs further study.

BACKGROUND:Recent studies have found that bone marrow mesenchymal stem cels that culturedin vitro for a long time can naturaly differentiate into neural stem cels, which then differentiate into neurons and glial cels, thereby providing a new therapeutic thinking for Parkinson’s disease, sequela of cerebral infarction, cerebelar atrophy and brain dysplasia.
OBJECTIVE:To discuss the influence of neural stem cel transplantation on neurologic function of rats with cerebral hemorrhage at recovery stage and the relevant mechanism of action.
METHODS: Sixty male Sprague-Dawley rats were randomly divided into normal group (n=18), cerebral hemorrhage group (n=21) and transplantation group (n=21). Cerebral hemorrhage models were established in the latter two groups using VII type colagen enzyme induction method. At 21 days of modeling, rats in the transplantation group were injected neural stem cels via the tail vein, and those in the other two groups received the same volume of normal saline. At 7, 14, 21 days after cel transplantation, modified adhesive removal test (MST) was employed to evaluate the neurologic function of rats, and then the rats were kiled. RT-PCR was used to detect angiopoietin-1 mRNA expression in the bleeding tissues, and western blot assay was employed to measure tyrosine kinase receptor-2 protein expression.
RESULTS AND CONCLUSION:Compared with the normal group, the MST scores in the cerebral hemorrhage group and transplantation group were significantly decreased (P< 0.05). From the 7th day after transplantation, MST scores in the transplantation group were significantly higher than those in the cerebral hemorrhage group (P < 0.05). At 7, 14, 21 days after transplantation, expressions of angiopoietin-1 mRNA and tyrosine kinase receptor-2 protein were ranked as folows: transplantation group > cerebral hemorrhage group > normal group, and there was a significant difference among the three groups (P< 0.05). These findings indicate that neural stem cel transplantation can effectively promote the neurologic recovery of rats with cerebral hemorrhage at recovery stage, and the concrete mechanism may be related to the increase of angiopoietin-1 mRNA and tyrosine kinase receptor-2 protein in the bleeding tissues.

Objective: To establish the functional occlusal morphology of fixed prostheses by using functional generated path (FGP) technology on a three-dimensional-printed diagnostic crown in order to decrease the chance of occlusal adjustment during try-in procedure of the complete crown, and to avoid the risk of occlusal interference after cementation of prosthesis. Methods: A double-blind clinical trial was performed. Ten subjects (4 males, 6 females) who needed a first molar complete crown restoration were enrolled in Department of Prosthodontics, Peking University School and Hospital of Stomatology from July 2018 to December 2018. The working models with the abutment teeth of the ten participants were scanned to obtain the digital models, then the occlusal surfaces of crown were formed by either FGP technology on a three-dimensional (3D) printed diagnostic crown and then were scanned directly for computer aided design and computer aided manufacture (CAD/CAM) crown fabrication as experiment group, or conventional technology based on database as control group. Then zirconia crowns were fabricated for two groups. During crown try-in of the two groups, preferred crowns of subjects were recorded, the occlusal contact criteria including the occlusal contact area (mm²), the heights of occlusal high spot (mm) before and after the adjustment of crowns were digitally analyzed, and the amount of adjustment (mm and mm³) were calculated on digital models by using a reverse engineering software (Giomagic Sudio & Quality). Differences of values were compared statistically with paired t-test (α=0.05). Results: The amount for occlusal adjustment for crowns in the experiment group [(7.320±4.238) mm³] was statistically less than that in the control group [(20.178±9.650) mm³] (P<0.05), the occlusal contact area of experiment group [(11.430±4.102) mm²] was statistically bigger than that of the control group [(4.808±3.223) mm²] (P<0.05), the heights of occlusal high spots for crowns in the experiment group was (0.043±0.019) mm while it was (0.594±0.201) mm in the control group with statistically significant differences (P<0.05). Conclusions This method could lead to a crown with less adjustment of occlusion, less occlusal high spot and less risk of occlusal interferences.