Objective To investigate the family function and features of parental attachment in children with tic disorders ,and to provide evidence for their family intervention. Methods Fifty three children with tic disorders and eighty five normal controls were investigated by family adaptability and cohesion scales, second edition, Chinese version (FACES Ⅱ-CV) and attachment security scale(Kems Security Scale). Results Actual cohesion scores,actual adaptability scores and ideal adaptability scores of the tic disorders children were all lower than those controls((66.87 12.53) vs (72. 14 11. 05) , (44. 19 9.70) vs (49.01 9.19), (52. 38 9. 10) vs (57.10 8.60), P<0.05, P<0.01); the ratio of the Disengaged type of cohesion in the tic disorders children was higher than that of normal controls(25.5% vs 5.9% , P<0.01) ;the ratio of the Rigid type of adaptability was higher than that of normal controls(55.3% vs 25.9% , P<0.01) ;the ratio of the Flexible type of adaptability was lower than that of normal controls( 12.8% vs 30.6% , P<0.05) ;the proportion of the type of extreme in the tic disorders children was higher than that of normal controls(40. 4% vs 23. 5% , P<0. 05). Father-dependence, mother-dependence, mother-intimacy, father-child attachment, mother-child attachment were all lower than those controls. In cases,positive correlation was found not only between actual cohesion scores and father-dependence, mother-dependence, mother-intimacy, father-child attachment, mother-child attachment, but also between actual adaptability scores and the above-mentioned five factors (0.291≤ r≤0.445,0. 301 ≤ r ≤0. 504). Mother-child attachment as an independent variable was separately leaded into the multiple progressively regression model for actual cohesion scores and actual adaptability scores(P<0.01). Conclusion The family function and parental attachment security of the tic disorders children were lower than the controls.

Objective To explore the clinical effect of lateral-rectus approach for treatment of acetabular fracture combined with ipsilateral pelvic fracture. Methods From January 2010 to February 2014, 24 patients with acetabular fracture combined with ipsilateral pelvic fracture were surgically managed through the lateral-rectus approach. Eighteen cases of merely anterior acetabular and pelvic fractures were treated with the lateral-rectus approach only, in the supine position. Six cases involving the posterior acetabular wall and posterior column were treated with the lateral-rectus approach combined lateral-rectus and posterior Kocher-Langenbeck approaches, in the floating position. The titanium reconstruction plates and cannulated screws were applied in these cases. Results The operation time was 70- 185 min and the intraoperative blood loss was 420-1 100 ml. Postoperative X-ray and CT exams showed excellent and good reduction. According to the postoperative Matta radiological evaluation, reduction of acetabular fracture was rated as anatomic in 13 cases, with satisfactory reduction in 8 cases and unsatisfactory in 3 cases. Injury to the sciatic nerve occurred in one case, and fat liquefaction at the abdominal incision in one case. All the patients were followed up for 13-30 months and the fractures healed after 10-16 weeks. According to the modified Merle d'Aubigne and Postel scoring system, 16 cases were excellent, 6 cases were good and 2 cases were fair 6 months after operation. Conclusions Surgical management of acetabular fractures through the lateral-rectus approach can provide adequate exposure and treatment for not only the acetabular fractures involving the anterior column and the quadrilateral surface, but also ipsilateral fractures involving the pubic superior ramus, iliac ala and sacroiliac joint, leading to fine outcomes.

Oncosis is another form of cell death, which is different from apoptosis. The review will discuss the recent advances of oncosis on pathological morphology, nuclear biochemical changes and molecular mechanisms. [

Fluorescence imaging of animal in vivo is to tag cell or DNA with fluorescence reporter gene, and then detect the fluorescence using sensitive optics apparatus. With this system, researcher can observe tumor growth, metastasis and angiogenesis in vivo, and reliable information for tumor diagnosis and treatment will be provided for researcher.

Near-infrared technology can be used as an auxiliary means for the early diagnosis of cancer and for surgical resection of tiny lesion that can not be discriminated by naked eyes,but it can not satisfy the purpose when used alone.Thus,we have developed an integrated technique by combining near-infrared and other technologies,and extended the scope of its application.This article reviews the advances of several integrated techniques applied to tumor research,including double labeled technology of nuclide and near-infrared,the combination of near-infrared and fluorescence resonance energy transfer,the combined use of near-infrared and endoscopy,and the integration of near-infrared with carbon nanotubes.

[Objective] To research the plantar pressure distribution of the normal Chinese adult. [Method] The F-scan plantar pressure analysis system was used to collect plantar pressure distribution data of 100 volunteers who stand, walk, jog, go upstairs and downstairs.The plantar pressure distribution data of 5 kinds of physiological states, as well as influence of sex, height, body weight, body mass index and speed was analyzed. [Result] There's different characteristic between 5 kinds of states of the plantar pressure distribution in the Chinese normal adults. There's significant difference between static and dynamic. In four dynamic motions, the gait of walk was stalest, and it became unstable if speed up and go upstairs or downstairs. As for the influential factors of the plantar pressure distribution, there was non-significance of sex. But the speed had the significance. The height, the body weight, the BMI had weak correlation with the plantar pressure distribution. [Conclusion] The plantar pressure distribution of normal Chinese adults has the distinctive quality. The data obtained by this experiment, may provide the reference for the clinical plantar pressure analysis.

Objective To explore agreement between parent-child report of self-rating scale of systemic family dynamics (SSFD),as well as factors that may influence the agreement.Methods SSFD was used to investigate the agreement between parent and child reports on family dynamics in a sample of 639 Chinese children aged 10 to 18 years from Mainland China,and possible influence factors.Results The results showed that there was significant difference between parent-child report of SSFD.The parents had significant lower score than their children at atmosphere,individuation and disease conception and had higher score at system logic (all P＜0.01).But the two still had significant positive correlation (correlations ranging from 0.15 to 0.38),indicating a low to moderate agreement between them(r=-0.15-0.38,all P＜0.01).The age factor was related to parent-children agreement on SSFD.Parent-child agreement was highest for family atmosphere,followed by individuation,system logic and disease conception.The age factor was related to parent-child agreement on SSFD.Conclusions Both parents' and children's reports of SSFD can be used to assess family dynamics,however,attention should be payed to the affection of parent-child report's difference and age factor to the results.

Objective To study the effect of intra-pulmonary arterial anti-tumor necrosis factor-α antibody(TNF-α Ab) on ultra-structure of lung after cardiopulmonary bypass (CPB).Methods Forty New Zealand rabbits were selected and randomly divided into four groups:group Ⅰ-Ⅲ underwent CPB; group Ⅳ only received open chest operation.In the group Ⅱ,rabbit TNF-α Ab(27 ng/kg) was dropped into the pulmonary artery when the aorta was clamped and CPB continued 30 minute.Pulmonary arterial perfusion was given to the group I instead.Blood TNF-α and neutrophils count from right and left atrium and oxygenation index in the four groups were determined perioperatively.Lung water content,TNF-αmessenger RNA,Bcl-2 protein,Bax protein,Bcl-2/Bax,apoptosis index and pathomorphological change were measured in the lung tissues.Results TNF-α Ab can restrain leukocyte accumulation and reduce releasing of TNF-α in the lung and improve oxygenation index.Moreover,TNF-α Ab can reduce the expression of Bax on alveolar epithelial cells and alleviate the decreasing of the expression of Bcl-2.It can also reduce the occurrence of apoptosis and attenuate pathomorphological changes in the lung tissue.Conclusion Intra-pulmonary arterial antitumor necrosis factor-α antibody markedly lessens the injury of inflammatory reaction and ultra-structure of lung after CPB via Bcl-2/Bax pathway.

ObjectiveTo explore the safety and therapeutic effect of 8 patients with esophageal Hiatal Hernia treated by laparoscopic hernia repair.MethodsA retrospective analysis was performed on the clinical data of 8 patients with esophageal Hiatal Hernia form Jun.2009 to Jun. 2010.Among the participants,3 conducted 360-degree fundoplication,5 conducted partial(270-degree) fundoplication.Silk sutures were used for the repair of esophageal perforation in 4 patients,and patch repair was used for the other 4 cases.ResultsEight patients were treated by laparoscopic hernia repair,and all of them were cured without postoperative complications.The mean duration of surgery was ( 120 ± 30) min,with average blood loss ( 50 ± 12 ) ml.Patients had a mean postoperative hospital stay of(4.5 ± 2.5 )days.All the patients were followed up for 1 to 2 years,and no case was found to be relapsed.ConclusionTotal laparoscopic hernia repair is minimally invasive,with short recovery course,less pain after surgery,little complication and short hospitalized time.Laparoscopic Hernia repair should be the preferred effective operation method for patients with esophageal Hiatal Hernia.

AIM: To investigate the inhibitory effect of Co-SZ eye drops on apoptosis of lens epithelial cells(LEC) induced by H_2O_2 and to study the cellular and molecular mechanisms.METHODS:(1) All lenses of Sprague-Dawley rats were incubated with H_2O_2 and Co-SZ eye drops.The apoptosis rates of LEC were determined by TUNEL method.The changes of LEC ultrastructure and the formation of apoptotic body were observed by electron microscopy.(2) Bovine LEC were incubated with H_2O_2 and Co-SZ eye drops.The inhibitory of LEC apoptosis was detected by MTT after incubation.The changes of fractional DNA content in LEC were detected by flow cytometry(FCM).[Ca~(2+)]i,cAMP and cGMP of LEC were determined by spectrofluoremeter and radioimmunoassay,respectively.RESULTS: The LEC apoptosis rates in Co-SZ eye drops group were decreased significantly compared with H_2O_2 group by TUNEL.The ultrastructure changes in LEC of Co-SZ eye drops group were lighter than that in H_2O_2 group.The LEC apoptosis rates of Co-SZ eye drops group were dose-dependently decreased significantly compared with H_2O_2 groups via MTT assay.LEC apoptosis induced by H_2O_2 was inhibited by Co-SZ eye drops,and showing dose-dependent.The DNA contents in LEC of Co-SZ group were increased.The [Ca~(2+)]i and cAMP in Co-SZ group were decreased obviously.The cGMP was increased.CONCLUSION: The LEC apoptosis induced by H_2O_2 was inhibited by Co-SZ eye drops.The mechanism of apoptosis inhibition by Co-SZ eye drops maybe contribute to the increase in DNA content.The signal transduction mechanisms are related to the decrease in [Ca~(2+)]i and cAMP and the increase in cGMP.