Objective:To establish a trace chromogenic substrate method for the determination of anti-FIIαactivity in compound heparin sodium cream .Methods: The anti-FIIαactivity in compound heparin sodium cream was determined by a trace chromogenic substrate method according to the completely random design of experiment based on the amount reaction principle of 4*4 parallel lines in the biological test statistics method .Results:The calibration curve was linear within the range of 0.005 04 IU· ml-1-0.021 IU· ml-1(r=0.992).The average recovery was 101.6% with RSD of 2.76% (n=9).Conclusion: The method is accurate, reliable and reproducible , and can be used for evaluating the quality of compound heparin sodium cream .

Objective To examine the sensitivity and specificity of glycated haemoglobin A1c (HbA1c) in predicting metabolic syndrome (MS) and its association with cardiovascular risk factors.MethodsIn 6292 adults (median age 45 years) who participated in a medical check-up program,analysis of distribution of HbA1c and its association with various cardiovascular risk factors was performed. The ability of HbA1 c to predict MS was evaluated.Anthropometric measurements were made and fasting plasma glucose,lipid profiles and HbA1c were tested. ResultsThe prevalence of MS was 11.24％.Cardiovascular risk factors were significantly increased as the serum level of HbA1c increased. HbA1c of 5.8％ predicted the presence of MS.Females showed the same cut-off of HbA1c for the prediction of MS with males ( the area under the curve of the females was higher than that of the males ). Conclusion HbA1c was increased as cardiovascular risk factors increased and HbA1c of 5.8％ may predict the presence of MS.HbA1c might be a predictive measure of MS and cardiovascular diseases in adults.

Objective To survey the serum level changes of retinal binding protein 4(RBP4)in newly diagnosed type 2 diabetes,and to explore related factors of RBP4 level.Methods According to body mass index(BMI),95 newly diagnosed type 2 diabetes in Beijing Hospital from April to October of 2002 were divided into obese-diabetic group(BMI≥25),normal-weighted diabetic group(BMI

Objective To investigate the relationship between serum levels of adiponectin (APN) and retinol binding protein 4 (RBP4) in newly diagnosed type 2 diabetes mellitus (T2DM) patients. Methods 95 newly diagnosed T2DM patients and 55 non-diabetic volunteers from health examination population were studied. The serum concentrations of APN and RBP4 were determined by ELISA. The levels of plasma glucose, lipid profiles and insulin were also measured in fasting samples. Insulin sensitivity was assessed by insulin resistance index (HOMA-IR). The control group and diabetic group were divided into ≥60 years subgroup and <60 years subgroup respectively. According to the guideline designed by Chinese Diabetes Society(CDS)in 2004, the control group and diabetic group were divided into different kinds of abnormal metabolism groups. Results (1)The level of APN was significantly decreased in diabetic group compared with the control group[(7.26±4.69)mg/L vs. (11.93±4.89)mg/L], while the serum level of RBP4 in diabetic group was obviously higher than that in control group[(16.48±7.82) mg/L vs. (10.91±5.26) rag/L]. (2) The prevalence of metabolic syndrome in diabetic group was higher than that in control group (46.3% vs. 7.3%). (3)APN level was decreased with the increasing number of components of MS, and RBP4 level was increased with the increasing number of components of MS. (4) In DM group, APN level was correlated negatively with MS, waist circumference and BMI, and RBP4 level was correlated positively with waist circumference, HOMA-IR, BMI and TG. Conclusions APN and RBP4 levels are significantly associated with metabolic syndrome. Hypoadiponectinemia and higher retinal binding protein 4 may play an important role in the pathogenesis of MS.

Objective To evaluate the professional competency of the clinicians with DRGs indexes. Methods The homepages of medical records of the hospital from January to June in 2018 were extracted, and the conventional evaluation indexes ( i. e. percentage of drug expenditure, percentage of medical consumables expenditure, and percentage of antimicrobials application ) of each clinician were obtained. " CN-DRGs" burster was used to calculate the DRGs indexes. The weighted RSR was applied to evaluate the professional competency of the resident physicians. Results A comprehensive evaluation of the professional competency of 438 resident clinicians was conducted. 218, 190, and 30 clinicians fell into excellent, medium and poor categories respectively. The results of ANOVA showed that the differences of the 3 categories were significant ( F =664. 863, P <0. 001 ). Conclusions The evaluation of clinicians′professional competency based on DRGs was reasonable, objective and feasible, proving its importance for the establishment of a scientific and standardized clinicians evaluation system.

Objective To compare the efficacy and toxicity of paclitaxel and carboplatin regimen concurrent chemoradiotherapy and radiotherapy alone in the treatment of stage Ⅲ cervical cancer.Methods A retrospective analysis of 158 patients with stage Ⅲ cervical cancer who were admitted to Shanxi Provincial Cancer Hospital from May 2009 to October 2012 was conducted.According to different treatment methods,86 patients were in the concurrent chemoradiotherapy group and 72 patients were in the radiotherapy group.The efficacy and adverse reactions of the two groups were compared.Results The effective rate (complete remission + partial remission) in the concurrent radiotherapy group and radiotherapy group was 95.3％ (82/86) and 84.7 ％ (61/72),respectively,and the difference was statistically significant (x2 =5.15,P =0.023).The 1-,2-,3-,and 5-year survival rates of the concurrent radiotherapy group and radiotherapy group were 93.0 ％ (80/86),77.9 ％ (67/86),68.6 ％ (59/86),60.5 ％ (52/86),and 81.9 ％ (59/72),61.1％ (44/72),41.7 ％ (30/72),36.1％ (26/72),respectively,and the differences were statistically significant (x2 values were 4.55,5.29,11.56,9.30,all P ＜ 0.05).The short-term adverse reactions in the concurrent chemoradiotherapy group and radiotherapy group were mainly myelosuppression and gastrointestinal reactions,and the incidence of myelosuppression was 87.2 ％ (75/86) and 50.0 ％ (36/72),respectively,and the difference was statistically significant (x2 =25.96,P ＜ 0.01);the incidence of gastrointestinal reactions was 91.9 ％ (79/86) and 20.8 ％ (15/72),respectively,and the difference was statistically significant (x2 =82.04,P ＜ 0.01).The incidence of radiation proctitis in the concurrent radiotherapy and chemotherapy group was 17.4 ％ (15/86) and 16.7 ％ (12/72),respectively,and the difference was not statistically significant (x2 =0.017,P =0.89);the incidence of radiation cystitis was 7.0 ％ (6/86) and 5.6 ％ (4/72),respectively,and the difference was not statistically significant (x2 =0.134,P =0.71).Conclusion Paclitaxel and carboplatin regimen chemotherapy combined with radiotherapy can improve the survival rate of patients with stage Ⅲ cervical cancer,and the adverse reactions are tolerable.

Objective:To analyze the clinical effect, adverse events and prognosis of microwave hyperthermia combined with radiotherapy in the treatment of patients with advanced gastric cancer.Methods:From May 2017 to May 2019, 80 patients with advanced gastric cancer admitted to Cancer Hospital of Shanxi Province were selected and divided into two groups according to the random digital table method, with 40 cases in each group.The control group was treated with radiotherapy, and the study group was treated with microwave hyperthermia on the basis of the control group.The clinical efficacy, adverse events and prognosis of the two groups were compared.Results:The total effective rate in the study group was 90.00%(36/40), which was higher than 70.00%(28/40) in the control group (χ 2=5.000, P<0.05). After treatment, the diameter of tumor in the study group was lower than that in the control group[(2.30±0.20)cm vs.(2.66±0.23)cm, t=7.470, P<0.05]. The incidence of adverse events in the study group was 10.00%(4/40), which was lower than 27.50%(11/40) in the control group(χ 2=4.020, P<0.05). The quality of life scores of the study group[physiological function (74.17±5.23)points, physical pain (73.07±5.49)points, general health (75.13±5.50)points, mental state (75.16±3.27)points and social function (75.49±3.24)points] were better than those of the control group[(62.55±5.70)points, (63.24±6.37)points, (65.72±5.30)points, (63.05±2.49)points, (65.34±4.59)points]( t=9.500, 7.393, 7.791, 18.634, 11.425, all P<0.05). Conclusion:Microwave hyperthermia combined with radiotherapy can reduce adverse events and improve the quality of life of patients with advanced gastric cancer.

Objective:To investigate the efficacy and safety of ixazomib-based therapy for multiple myeloma.Methods:The data of 32 patients with multiple myeloma treated with isazomib-based regimen in the Affiliated Hospital of Jining Medical University from December 2020 to December 2021 were retrospectively analyzed. Among 32 patients, 17 cases were relapsed/refractory, and the remaining 15 cases had initial treatment. The treatment regimens included ID (isazomib + dexamethasone), IRD (isazomib + lenalidomide + dexamethasone) and ICD (isazomib + cyclophosphamide + dexamethasone). The short-term curative effect and adverse reactions of relapsed/refractory patients and patients at initial onset were analyzed.Results:The overall response rate (ORR) of relapsed/refractory patients was 52.9% (9/17), of which 6 cases achieved complete remission (CR), 2 cases achieved very good partial remission (VGPR) and 1 case achieved partial remission (PR). The ORR of refractory patients receiving bortezomib therapy was 40.0% (4/10). The ORR of patients at initial onset who could be evaluated the curative effect was 100.0% (14/14), including 9 cases of CR, 2 cases of VGPR and 3 cases of PR. After treatment, 2 patients (6.2%) had grade Ⅲ-Ⅳ adverse events (1 case of herpes zoster and 1 case of thrombocytopenia), and none of the patients had grade Ⅲ-Ⅳ peripheral neuropathy.Conclusion:Isazomib is effective and safe in the treatment of initially treated and relapsed/refractory multiple myeloma.

Objective To induce experimental immune tole-rance in rabbits and observe its effects on heterotopic cardiac transplantation.Methods Donor's splenic lymphocytes pre-treated with platinum metal chelator were injected into the recipient's mesenteric-portal vein. Cyclosporin A was perfused through the donor's heart. Results The injection of donor's splenic lymphocytes before transplantation could significantly prolong the survival time of the heterotopically transplanted heart. The effect of two injections was better than that of one. Radioactive tracer studies showed that the 99m Tc-HMPAO tagged lymphocytes injected into the recipient rabbit were later concentrated in the liver, though initially they were distributed in multiple organs. The induced immune tolerance was antigen-specific, and it neither affect the other immune functions of the lymphatic system prominently nor exert any harmful effect on the recipient's liver and renal functions. The perfusion of cyclosporin A through the donor heart could block the glycosyl groups, such as D-glucose, D-mannose or N-acetyl-galactosamine on the surface of the myocardial cells, thus might change the antigenic expression, effectively preventing rejection of the graft by the host, and might be considered as a new method to block graft rejection in cardiac transplantation. The combined use of the above-mentioned two methods acted on both the host and the donor, thus reducing the exposed antigens on the donor organ as well as the immune reaction against the donor antigens, and resulting in synergistic effect in inducing immune tolerance in adult rabbits, and resulting in relatively long-term survival of transplanted hearts. Conclusion This report may provide the experimental basis for inducing immune tolerance in clinical transplantation.

The Mayo Clinic in the United States is a non-profit medical institution that integrates clinical practice, education, and research. It has maintained a global top hospital brand image for a long time and its management ideas have been widely recognized and appreciated by the industry. From the perspective of strategic management, this study elaborated on the core values of Mayo Clinic, the multidisciplinary team cooperation diagnosis and treatment model, the high fixed salary salary system, the management model of doctor leadership-operation supervisor cooperation, the business mission of integrated clinical practice, education, and research, and the management points of digital marketing, which could provide references for the high-quality development and brand building of hospitals in China.