Objective With eukaryote expression vector pEGFP-C2, to establish the HepG2 cell line which may stably express the P22e of hepatitis B virus (HBV), and may be used to study the existed relationship between HBVP22e and hepatitis B. Methods HBVP22e cDNA obtained by PCR from HBV adr subtype 1.2 copies genome plasmid p3.8II was inserted into universal vector pMD18-T for identification. The plasmids were transfected into HepG2 cells via liposome, while EGFP-HBVP22e was analyzed by Western blot, and observed with fluorescence microscope in HepG2 cells. Results Expression vectors of recombinant pEGFP-C2HBV P22e were constructed and expressed steadily in HepG2 cells. Conclusion The results can be used to explore biological activity of HBVP22e in hepatitis B.

Objective:To compare body mass index (BMI) of freshmen from 2012 to 2015, to reveal the existence of endemism. Methods:Literature research, mathematical statistics, comparative analysis and other methods were used to analyze BMI of Shenyang Medical College from 2012 to 2015. Results:The average BMI had a significant difference in nearly four years (P<0.05) . While BMI of male freshmen in nearly four years had no difference (P>0.05), but not in female (P<0.05) . Conclusion:From 2012 to 2015,basic distribution of BMI of college freshmen is normal,but BMI in the standard range decreased year by year,the proportion of overweight and obesity is increasing,while BMI of female freshmen has a tendency to decrease.