1.Effect of Retroviral Vector-Mediated IL6/IL2 Fused Gene Transduction on B16 Melanoma Cells Adhesive and Metastatic Potential
Xiaodan YU ; Peixian TANG ; Mingwei ZHANG
Chinese Journal of Cancer Biotherapy 1995;0(02):-
In order to examine the feasibility of cytokine fusion gene transfer into tumor cells, a retroviral vector (pLXSN) for human interleukin 6/interleukin 2 (IL6/IL2) fusion gene was constructed by using PCR and ligating the 2 genes with a synthesized flexible linker. The IL6/IL2 fused gene was introduced into B16 melanoma cell line mediated by retrovirus and the changes of adhesive and metastatic ability of fused gene-modified cells were detected. The B16-IL6/ IL2 cells showed efficient expression of both cytokines and decreased binding affinity with extracellular matrix (Laminin and Matrigel), accompanying with decreased experimental metastatic potentials. These data suggest that the mechanism involving the decreased metastases may relate with the changes of biological characteristics and immune stimulating activity of gene-modified cells.
2.Oligodendroglial cytoplasmic inclusion in multiple system atrophy
Mingwei ZHU ; Luning WANG ; Hongchuan TANG
Chinese Journal of Neurology 2001;0(02):-
Objective To investigate the oligodendroglial cytoplasmic inclusion (OCI) in the central nervous system of multiple system atrophy (MSA) and to evaluate its roles in pathologic diagnosis of MSA.Methods Modified Gallyas Braak staining was used to investigate tissue samples of the brain and the spinal cord of 4 cases with MSA, which were previously diagnosed by clinical and routine pathologic methods. Eight cases with motor neuron disease and 6 cases without nervous system disease were used for control study.Results OCIs were demonstrated by Gallyas barrak method in the white matter of the brain and the spinal cord of 3 cases with MSA. They look like sickle , semilunar , and flame shaped. Their main distributions were transverse fibers of the basis pontine, the white matter of the cerebellum, striatopallidal fibers and lateral column of the spinal cord. While OCIs were not found in the brain and the spinal cord of one case without typical clinical features and pathologic findings. Nor did all of the control cases.Conclusions OCIs may be regarded as pathologic marker of the sporadic forms of MSA. It also suggests that OCIs are related to degenerative process of myelinated fibers in MSA.
3.Effect of oral antidiabetic drugs on NGF mRNA expr(e)ssion in rats brain with diabetes
Mingwei TANG ; Yao LI ; Fengbo WANG
Chongqing Medicine 2017;46(8):1017-1019
Objective To observe the expression of NGF mRNA in rats brain with diabetes and the effects of hypoglycemia caused by oral antidiabetic drugs on NGF mRNA.Methods A total of 64 adult Wistar rats were randomly divided into control group(16)and diabetes model group(48).Using high fat and high sugar diet+intraperitoneal injection of streptozotocin to prepare type 2 diabetes model.The diabetes model group was randomly divided into non-medication group(16),oral medication group (16) and hypoglycemia group(16).The oral medication rats and hypoglycemia rats were given glibenclamide+metformin by intragastric administration once daily for 12 d,the dose of hypoglycemia rats was 5 times of treatment rats.The brain tissues of rats in each group were used respectively the RT-PCR method to detect NGF mRNA at 3,6,9 and 12 d.Results Compared with the control group,the expression of NGF mRNA in the brain tissues of the 3 groups of diabetic rats were decreased in different degrees and the non-medication group was the most obvious(P<0.01).Compared with the non-medication group,the oral medication group was significantly increased(P<0.01),the hypoglycemia group was further decreased(P< 0.01).Conclusion Oral hypoglycemic drugs can effectively enhance the expression of NGF mRNA in brain tissue of diabetic rats,but hypoglycemia caused by Oral antidiabetic drugs can make the expression of NGF mRNA further decreased.So hypoglycemia is not conducive to the repair of diabetic neuropathy.
4.Effects of Electroacupuncture Combined with Enriched Rehabilitation Training on Expression of Brain-derived Neurotrophic Factor in Cerebral Cortex of Neonatal Rats with Brain Injury
Fengbo WANG ; Mingwei TANG ; Xiaojie LI
Chinese Journal of Rehabilitation Theory and Practice 2012;18(8):717-720
Objective To explore the effects of electroacupuncture combined with enriched rehabilitation training on the expression of brain-derived neurotrophic factor (BDNF) in the cerebral cortex of neonatal rats with brain injury. Methods Lipopolysaccharide (LPS, 450 μg/kg∙d) was injected to pregnant rats on the 17th and 18th days intraperitoneally. The control group was injected with equal sterile normal saline. The neonatal rats injected with LPS were randomly divided into non-intervention group and intervention group. The intervention group received intervention once a day until the 28th day. The control group and the non-intervention groups were fed regularly. Immunohistochemistry (SABC) was used to detect the expression of BDNF on the 1st, 14th, 21st and 28th days. Results The expression of BDNF was higher in the non-intervention group than in the control group (P<0.01) on the 1st day, but there was no significant difference on the 14th, 21st and 28th days. And it was higher in the intervention group than in the other 2 groups (P<0.01). Conclusion Electroacupuncture combined with enriched rehabilitation training can significantly improve the expression of BDNF in cerebral cortex of neonatal rats with brain injury
5.Relationship study of the expressions of Slit2 and Nogo-A in rats with cerebral hemorrhage
Fengbo WANG ; Qiongfen WANG ; Hong ZHANG ; Fei LI ; Mingwei TANG
Journal of Chinese Physician 2015;17(2):195-197
Objective To investigate the expressions of Slit2 and Nogo-A in the ipsilateral hippocampus after cerebral hemorrhage in rats.Methods A total of 64 adult Sprague-Dawley (SD) rats was randomly divided into control and model groups.Collagenase Ⅶ was used to induce cerebral hemorrhage model.Immunohistochemistry was used to detect the expressions of Slit2 and Nogo-A in hippocampus at the cerebral hemorrhage side at the time points (24 h,7 d,14 d,and 21 d).Results Compared to the control group,the expressions of Slit2 and Nogo-A were significantly enhanced in model group (P <0.01),with the highest level at the 7 d.Conclutions Cerebral hemorrhage can significantly enhance expressions of Slit2 and Nogo-A with a positive correlation of Slit2 and Nogo-A,which might have an important effect on the recovery of brain injury.
6.Effects of carbohydrate-electrolyte solution on serum glucose, pancreas islet function, and safety in elderly patients after abdominal operation
Qi AN ; Mingwei ZHU ; Hongyuan CUI ; Puxian TANG ; Danian TANG ; Xinping ZHOU ; Junmin WEI
Chinese Journal of Clinical Nutrition 2010;18(3):149-152
Objective To evaluate the effects of carbohydrate-electrolyte solution(CES)on serum glucose,pancreas islet function,and safety in elderly patients after abdominal operation.Methods In this prospective,double-blinded,randomized,and controlled study,40 elderly patients who met the defined criteria were enrolled.Subjects in CES group were intravenously administered with 1 000 ml CES for consecutive three days beginning from the 1st and 2nd post-operative day,while subjects in the control group were administered with 10% glucose of the same volume under the same arrangement.The changes of serum glucose,insulin and insulin C-peptide,as well as lactic acid and uric acid and uric acid were determined before and after injection.Adverse events were recorded.Results All patients completed the study.The increase rate of serum glucose was significantly lower on the 2nd and 3rd day after injection in CES group than in control group(P=0.008,P:0.001).Blood insulin and insulin C-peptide levels showed increasing trends in both two groups,but were not significantly different between two groups(P=0.612,P=0.213).In the CES group,6 patients experienced systemic inflammatory response syndrome and 4 patients had infective complications after surgeries ;on the contrary,these two numerals were 8 and 6 in the control group(P=0.639,P=0.606).No increase in serum lactic acid or uric acid was detected.Conclusion Appropriate application of CES has minimal effect on the blood gluocse and pancreas islet function in elderly patients after abdominal surgery and may be helpful to improve clinical outcomes.
7.Prevalence of nutritional risk and malnutrition among hospitalized elderly abdominal surgical patients with malignant tumors
Guodong YE ; Mingwei ZHU ; Hongyuan CUI ; Danian TANG ; Qi AN ; Jifang MEN ; Junmin WEI
Chinese Journal of Clinical Nutrition 2011;19(6):364-367
ObjectiveTo investigate the prevalence of nutritional risk and malnutrition among hospitalized elderly abdominal surgical patients with malignant tumors.MethodsTotally 269 elderly patents ( ≥ 65 years) with malignant tumor who were hospitalized in our department of abdominal surgery from December 2009 to November 2010 were consecutively enrolled.Nutritional Risk Screening 2002 ( NRS 2002 ) was performed on the next morning after admission.Body mass index (BMI) lower than 18.5 kg/m2 was considered as malnutrition.Results The NRS 2002 was completed in all the 269 enrolled patients.The overall prevalence of malnutrition was 30.1% (81/269) ; more specifically,37.5% (21/56) among geriatric patients ( ≥80 years) and 17.6% (43/245) among the other age groups (P =0.001 ).The overall rate of nutrition risk was 38.3% (103/269) ; more specifically,57.1% (32/56) among the geriatric patients and 29.3% (72/245) among the other age groups (P <0.001 ).The rate of nutrition risk in patients with pancreas cancer was 58.3%,which was higher than other elderly patients; on the contrary,and the rate of nutrition risk in the patients with colorectal cancer was relatively lower.ConclusionThe rates of nutrition risk and malnutrition in hospitalized elderly abdominal surgical patients ( ≥ 65years) with malignant tumor are relatively higher than other age groups,especially among the geriatric patients.
8.Clinical outcome of the combined nutritional support for colorectal cancer patients at nutritional risk: a retrospective study of 60 cases
Danian TANG ; Mingwei ZHU ; Jianhua SUN ; Qi AN ; Hongyuan CUI ; Jifang MEN ; Junmin WEI
Chinese Journal of Clinical Nutrition 2011;19(6):355-359
ObjectiveTo explore the clinical outcome of the combined nutrition support for colorectal cancer patients.MethodsTwo research arms were obtained using a cohort sampling method.Ann A ( the study group): from 2009 to 2010,30 colorectal cancer patients were enrolled.They received nutritional risk screening (NRS) 2002 after admission.Nutritional risk was defined as NRS 2002 score ≥3 three days before operation.Patients with nutritional risk received enteral nutrition (EN) for bowel preparation without laxative drug and enema.After operation,they received EN combined with parenteral nutrition (PN) supports provided.Arm B (control group): 30 cases with historically confirmed colorectal cancer were enrolled from 2007 to 2008.They received routine bowel preparation (diet control,laxative drug,and enema) and PN supports after operation.Nutritional parameters,the rate of infectious complications,the rate of systemic inflammatory response syndrome,and the duration of hospital stay were analyzed.ResultsThere were no significant difference in body weight and plasma albumin between these two arms ( P > 0.05 ). The incidence of systemic inflammatory response syndrome (13.3 % ),infectious complications (10.0% ),and the duration of hospital stay [ (12.3 ± 6.5 ) d ] in arm A were significantly lowerthan those in arm B [33%,30%,and (15.0 ±7.2) d,respectively] (P =0.038,P =0.042,P =0.045).Conclusion For the colorectal cancer patients,nutritional risk screening on admission,bowel preparation with eneral nutrition before operation,and combined nutritional support after operation can improve the clinical outcome.
9.Analysis of death cases in elderly patients with digestive tract disease during perioperative period
Danian TANG ; Junming WEI ; Mingwei ZHU ; Meixiong HUANG ; Bei WU ; Yongguo LI
Chinese Journal of Geriatrics 2001;0(01):-
renal dysfunction.Logisitic regression showed that cardiovascular disease and hypoalbuminemia were correlated with perioperative death rate significantly. Conclusions Improving the perioperative management is very important for lowering the perioperative mortality in elderly patients with digestive tract disease.
10.The effect of hyperbaric oxygen treatment on plasma endotoxin,sCD14 and plasma endotoxin inactivation capacity in acute necrotizing pancreatitis in rats
Danian TANG ; Yongguo LI ; Mingwei ZHU ; Junming WEI ; Bei WU ; Xiuwen HE
Chinese Journal of General Surgery 1997;0(06):-
Objective To study the effect of hyperbaric oxygen(HBO) treatment on plasma endotoxin,sCD14 and plasma endotoxin inactivation capacity(EIC) in acute necrotizing pancreatitis(ANP) in rats and its possible mechanism.Methods SD rats were randomly divided into control group,sham operation group,ANP group and ANP+HBO group.Rat ANP models were made by retrograde injection of 3.5% sodium(taurocholate)(2.5mL/kg) into the pancreatic duct.Thirty minues after models had been made,ANP+HBO group was treated by hyperbaric oxygen for 2h.At 3h,6h,and 10h after establishment of rat models,the plasma endotoxin,sCD14,EIC,TXB_2 and 6-K-PGF_(1a) leves were determined in each group.Results At 3h and 6 h after rat models were established,the levels of endotoxin,sCD14,and TXB_2 in ANP+HBO group were significantly lower than those in ANP group(P